Histopathology H&E Staining: Principle and Interpretation

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15 Questions

What is the principle behind H&E stain?

Attraction between tissue and dye

What is the primary function of acidic eosin in H&E staining?

Counterstains basic elements

Which dye imparts blue-purple contrast on basophilic structures?

Hematoxylin

What is the purpose of using Xylene in the H&E staining procedure?

To deparaffinize the tissue

Which staining technique is the most widely used in histopathology?

Hematoxylin and Eosin (H&E) staining

What is the primary function of hematoxylin in H&E staining?

Imparts blue-purple contrast on basophilic structures containing nucleic acid moieties

What is the purpose of using Xylene in the H&E staining procedure?

Clears the tissue of paraffin and dehydrates it

What does acidic eosin primarily counterstain in H&E staining?

Basic elements such as RBCs, cytoplasm, muscle, and collagen

Which staining technique is the most widely used in histopathology?

Hematoxylin and Eosin (H&E) stain

What is the chemical attraction behind the H&E staining process?

Attraction between tissue and hematoxylin

What is the primary focus of histopathology?

Studying disease in a tissue section

What is the purpose of an incisional biopsy?

To diagnose lesions or tumors before final removal

What does a whole mount preparation involve?

Preparation of entire animal, e.g., fungus, parasite

What is the majority type of preparation in histology?

Sections

What type of tissue is obtained for an excisional biopsy?

Whole tumor or lesion

Study Notes

H&E Stain Principle

  • The principle behind H&E stain is based on the chemical attraction between the basic and acidic groups of the dyes and the tissue components.
  • Hematoxylin is a basic dye that binds to acidic components of the tissue, such as nucleic acids and Glycosaminoglycans.
  • Eosin is an acidic dye that binds to basic components of the tissue, such as proteins and carbohydrates.

Hematoxylin Function

  • The primary function of hematoxylin in H&E staining is to stain basophilic structures, such as nuclei, ribosomes, and rough endoplasmic reticulum, a blue-purple color.

Eosin Function

  • The primary function of acidic eosin in H&E staining is to counterstain acidophilic structures, such as cytoplasm, collagen, and muscle fibers, a pink color.
  • Acidic eosin primarily counterstains cytoplasm and connective tissue.

Xylene Purpose

  • The purpose of using Xylene in the H&E staining procedure is to dehydrate the tissue sections and make them transparent.

H&E Staining Technique

  • The most widely used staining technique in histopathology is the H&E stain.

Histopathology Focus

  • The primary focus of histopathology is to examine tissues microscopically to diagnose diseases.

Biopsy Types

  • An incisional biopsy involves the removal of a small sample of tissue from a larger lesion.
  • An excisional biopsy involves the removal of an entire lesion or mass.
  • The majority type of preparation in histology is the paraffin-embedded section.
  • A whole mount preparation involves mounting an entire tissue or organ on a slide.

Learn about the principle, procedure, and interpretation of the widely used Hematoxylin and Eosin (H&E) staining technique in histopathology. Explore the differential staining of tissue elements using hematoxylin and eosin dyes.

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