Histopathology H&E Staining: Principle and Interpretation

What is the principle behind H&E stain?

What is the primary function of acidic eosin in H&E staining?

Which dye imparts blue-purple contrast on basophilic structures?

What is the purpose of using Xylene in the H&E staining procedure?

Which staining technique is the most widely used in histopathology?

What is the primary function of hematoxylin in H&E staining?

What is the purpose of using Xylene in the H&E staining procedure?

What does acidic eosin primarily counterstain in H&E staining?

Which staining technique is the most widely used in histopathology?

What is the chemical attraction behind the H&E staining process?

What is the primary focus of histopathology?

What is the purpose of an incisional biopsy?

What does a whole mount preparation involve?

What is the majority type of preparation in histology?

What type of tissue is obtained for an excisional biopsy?

H&E Stain Principle

The principle behind H&E stain is based on the chemical attraction between the basic and acidic groups of the dyes and the tissue components.
Hematoxylin is a basic dye that binds to acidic components of the tissue, such as nucleic acids and Glycosaminoglycans.
Eosin is an acidic dye that binds to basic components of the tissue, such as proteins and carbohydrates.

Hematoxylin Function

The primary function of hematoxylin in H&E staining is to stain basophilic structures, such as nuclei, ribosomes, and rough endoplasmic reticulum, a blue-purple color.

Eosin Function

The primary function of acidic eosin in H&E staining is to counterstain acidophilic structures, such as cytoplasm, collagen, and muscle fibers, a pink color.
Acidic eosin primarily counterstains cytoplasm and connective tissue.

Xylene Purpose

The purpose of using Xylene in the H&E staining procedure is to dehydrate the tissue sections and make them transparent.

H&E Staining Technique

The most widely used staining technique in histopathology is the H&E stain.

Histopathology Focus

The primary focus of histopathology is to examine tissues microscopically to diagnose diseases.

Biopsy Types

An incisional biopsy involves the removal of a small sample of tissue from a larger lesion.
An excisional biopsy involves the removal of an entire lesion or mass.
The majority type of preparation in histology is the paraffin-embedded section.
A whole mount preparation involves mounting an entire tissue or organ on a slide.

Learn about the principle, procedure, and interpretation of the widely used Hematoxylin and Eosin (H&E) staining technique in histopathology. Explore the differential staining of tissue elements using hematoxylin and eosin dyes.