High Temperature Impact on Bacterial Cells Quiz
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Questions and Answers

What happens to bacterial proteins at high temperatures (>90°C)?

  • They replicate
  • They become more stable
  • They denature (correct)
  • They break down

What occurs to nucleic acids in high temperatures (>90°C)?

  • They denature
  • They become more resistant
  • They are destroyed (correct)
  • They replicate faster

What happens to cellular membranes at temperatures above 90°C?

  • They denature
  • They replicate
  • They become more flexible
  • They are destroyed (correct)

What happens to nucleic acids in high temperatures (>90°C)?

<p>Destruction (B)</p> Signup and view all the answers

What is observed at low temperatures (0-7°C) in terms of microbial metabolic rate?

<p>Reduction of microbial metabolic rate (B)</p> Signup and view all the answers

What is the term used to describe the reduction of microbial metabolic rate at low temperatures?

<p>Microstatic effect (A)</p> Signup and view all the answers

Which type of microbes are exceptions to the microstatic effect at low temperatures?

<p>Psychrotrophs (A)</p> Signup and view all the answers

What is the effect of slow freezing (-20°C) on the cellular and molecular structure of bacteria and fungi?

<p>It disrupts the structure due to ice crystals (A)</p> Signup and view all the answers

What is the impact of quick freezing (-70°C) on microbes?

<p>Microbes become dormant but are not killed (A)</p> Signup and view all the answers

What is the primary reason for the disruption of microbial and fungal structure during slow freezing?

<p>Formation of ice crystals (A)</p> Signup and view all the answers

What is formed when water ionizes due to ionizing radiation?

<p>Highly reactive hydroxyl radicals (C)</p> Signup and view all the answers

What do highly reactive hydroxyl radicals formed from water ionization primarily react with?

<p>Organic cellular components, especially DNA (C)</p> Signup and view all the answers

Which type of radiation leads to water ionization and the formation of highly reactive hydroxyl radicals?

<p>Gamma radiation (D)</p> Signup and view all the answers

What is the primary mechanism by which non-ionizing radiation damages bacterial DNA?

<p>Causing bonds between adjacent pyrimidine bases (thymine dimers) (C)</p> Signup and view all the answers

What is the specific effect of non-ionizing radiation on DNA replication?

<p>Inhibited DNA replication (C)</p> Signup and view all the answers

Which type of radiation is categorized as non-ionizing?

<p>Ultraviolet (UV) radiation (D)</p> Signup and view all the answers

What effect does desiccation have on bacterial growth and reproduction?

<p>Slows down bacterial growth and reproduction (D)</p> Signup and view all the answers

How does high pressure affect proteins and carbohydrates in bacteria?

<p>Alters proteins and carbohydrates (A)</p> Signup and view all the answers

Which factor alters bacterial growth by slowing down microbial activity?

<p>Osmotic pressure (B)</p> Signup and view all the answers

What happens to proteins and carbohydrates at high pressure?

<p>They are altered, leading to rapid inactivation of vegetative cells (C)</p> Signup and view all the answers

At low temperatures (0-7°C), what happens to microbial metabolic rate?

<p>It reduces, slowing down microbial activity (B)</p> Signup and view all the answers

What is the effect of high temperatures (>90°C) on nucleic acids?

<p>They are altered, leading to rapid inactivation of vegetative cells (A)</p> Signup and view all the answers

What happens to proteins and carbohydrates when exposed to high pressure?

<p>They are altered, leading to rapid inactivation of vegetative cells (C)</p> Signup and view all the answers

What is the primary impact of high pressure on vegetative cells?

<p>Rapid inactivation due to alteration of proteins and carbohydrates (D)</p> Signup and view all the answers

How do proteins and carbohydrates react to low pressure conditions?

<p>They are altered, leading to rapid inactivation of vegetative cells (B)</p> Signup and view all the answers

What is the primary focus of the process of sanitation?

<p>Reducing microbial contamination on inanimate objects (A)</p> Signup and view all the answers

Which process specifically targets the prevention of contact with microorganisms?

<p>Asepsis (D)</p> Signup and view all the answers

What is the main objective of the process of antisepsis?

<p>Removing microorganisms from skin, mucous membranes, and wounds (B)</p> Signup and view all the answers

What is the primary difference between disinfection and the action of disinfectants?

<p>Disinfection reduces or eliminates all pathogenic microorganisms except spores, while disinfectants kill vegetative forms of microorganisms but do not eliminate bacterial and fungal spores or non-lipid viruses (B)</p> Signup and view all the answers

What is the limitation of disinfectants in terms of microorganisms they can eliminate?

<p>They do not eliminate bacterial and fungal spores, and non-lipid viruses (C)</p> Signup and view all the answers

For what purpose are disinfectants commonly used?

<p>Disinfection of articles such as medical instruments, bed-clothes, air, hands, and excrements (A)</p> Signup and view all the answers

What does the term 'sterile' indicate?

<p>Complete freedom from all microorganisms and their spores (B)</p> Signup and view all the answers

What is the primary objective of sterilization?

<p>To make an article free of all microorganisms, including spores, viruses, and fungi (A)</p> Signup and view all the answers

What is the process of sterilization intended to achieve?

<p>Complete freedom from all microorganisms and their spores (A)</p> Signup and view all the answers

What is the term used to describe complete freedom from all microorganisms and their spores?

<p>Sterile (C)</p> Signup and view all the answers

What is the primary objective of the process of sterilization?

<p>To eliminate all microorganisms, including spores, viruses, and fungi (B)</p> Signup and view all the answers

What is the specific impact of sterilization on microorganisms?

<p>Kills or inactivates them, including spores, viruses, and fungi (A)</p> Signup and view all the answers

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