High Temperature Impact on Bacterial Cells Quiz

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37 Questions

What happens to bacterial proteins at high temperatures (>90°C)?

They denature

What occurs to nucleic acids in high temperatures (>90°C)?

They are destroyed

What happens to cellular membranes at temperatures above 90°C?

They are destroyed

What happens to nucleic acids in high temperatures (>90°C)?

Destruction

What is observed at low temperatures (0-7°C) in terms of microbial metabolic rate?

Reduction of microbial metabolic rate

What is the term used to describe the reduction of microbial metabolic rate at low temperatures?

Microstatic effect

Which type of microbes are exceptions to the microstatic effect at low temperatures?

Psychrotrophs

What is the effect of slow freezing (-20°C) on the cellular and molecular structure of bacteria and fungi?

It disrupts the structure due to ice crystals

What is the impact of quick freezing (-70°C) on microbes?

Microbes become dormant but are not killed

What is the primary reason for the disruption of microbial and fungal structure during slow freezing?

Formation of ice crystals

What is formed when water ionizes due to ionizing radiation?

Highly reactive hydroxyl radicals

What do highly reactive hydroxyl radicals formed from water ionization primarily react with?

Organic cellular components, especially DNA

Which type of radiation leads to water ionization and the formation of highly reactive hydroxyl radicals?

Gamma radiation

What is the primary mechanism by which non-ionizing radiation damages bacterial DNA?

Causing bonds between adjacent pyrimidine bases (thymine dimers)

What is the specific effect of non-ionizing radiation on DNA replication?

Inhibited DNA replication

Which type of radiation is categorized as non-ionizing?

Ultraviolet (UV) radiation

What effect does desiccation have on bacterial growth and reproduction?

Slows down bacterial growth and reproduction

How does high pressure affect proteins and carbohydrates in bacteria?

Alters proteins and carbohydrates

Which factor alters bacterial growth by slowing down microbial activity?

Osmotic pressure

What happens to proteins and carbohydrates at high pressure?

They are altered, leading to rapid inactivation of vegetative cells

At low temperatures (0-7°C), what happens to microbial metabolic rate?

It reduces, slowing down microbial activity

What is the effect of high temperatures (>90°C) on nucleic acids?

They are altered, leading to rapid inactivation of vegetative cells

What happens to proteins and carbohydrates when exposed to high pressure?

They are altered, leading to rapid inactivation of vegetative cells

What is the primary impact of high pressure on vegetative cells?

Rapid inactivation due to alteration of proteins and carbohydrates

How do proteins and carbohydrates react to low pressure conditions?

They are altered, leading to rapid inactivation of vegetative cells

What is the primary focus of the process of sanitation?

Reducing microbial contamination on inanimate objects

Which process specifically targets the prevention of contact with microorganisms?

Asepsis

What is the main objective of the process of antisepsis?

Removing microorganisms from skin, mucous membranes, and wounds

What is the primary difference between disinfection and the action of disinfectants?

Disinfection reduces or eliminates all pathogenic microorganisms except spores, while disinfectants kill vegetative forms of microorganisms but do not eliminate bacterial and fungal spores or non-lipid viruses

What is the limitation of disinfectants in terms of microorganisms they can eliminate?

They do not eliminate bacterial and fungal spores, and non-lipid viruses

For what purpose are disinfectants commonly used?

Disinfection of articles such as medical instruments, bed-clothes, air, hands, and excrements

What does the term 'sterile' indicate?

Complete freedom from all microorganisms and their spores

What is the primary objective of sterilization?

To make an article free of all microorganisms, including spores, viruses, and fungi

What is the process of sterilization intended to achieve?

Complete freedom from all microorganisms and their spores

What is the term used to describe complete freedom from all microorganisms and their spores?

Sterile

What is the primary objective of the process of sterilization?

To eliminate all microorganisms, including spores, viruses, and fungi

What is the specific impact of sterilization on microorganisms?

Kills or inactivates them, including spores, viruses, and fungi

Test your knowledge of the effects of high temperatures on bacterial proteins, nucleic acids, and cellular membranes in this quiz. Explore the process of denaturation and destruction, and understand the impact of extreme heat on bacterial cells.

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