Podcast
Questions and Answers
What is the reference range for hematocrit in adult males?
What is the reference range for hematocrit in adult males?
- 35 to 49%
- 30 to 45%
- 40 to 54% (correct)
- 50 to 60%
Which component remains at the bottom of the tube after centrifugation of blood?
Which component remains at the bottom of the tube after centrifugation of blood?
- Red blood cells (correct)
- Platelets
- White blood cells
- Plasma
Which type of capillary tube is used for anticoagulated blood specimens?
Which type of capillary tube is used for anticoagulated blood specimens?
- Blue band/ring tube (correct)
- Red band/ring tube
- Yellow band/ring tube
- Green band/ring tube
What is the maximum duration recommended for centrifugation to achieve complete packing of RBCs?
What is the maximum duration recommended for centrifugation to achieve complete packing of RBCs?
What should be excluded when reading the hematocrit results?
What should be excluded when reading the hematocrit results?
What is a common effect of trapped plasma on microhematocrit results?
What is a common effect of trapped plasma on microhematocrit results?
Which measurement of hematocrit typically considers RBC and MCV in its calculation?
Which measurement of hematocrit typically considers RBC and MCV in its calculation?
What technical issue could affect the validity of the hematocrit measurement when using capillary tubes?
What technical issue could affect the validity of the hematocrit measurement when using capillary tubes?
Flashcards
Hematocrit Definition
Hematocrit Definition
Hematocrit is the percentage of red blood cells (RBCs) in a blood sample. It measures the packed cell volume (PCV).
Hematocrit Reference Range (Adult Males)
Hematocrit Reference Range (Adult Males)
The normal hematocrit range for adult males is 40% to 54%.
Hematocrit Reference Range (Adult Females)
Hematocrit Reference Range (Adult Females)
The normal hematocrit range for adult females is 35% to 49%.
Microhematocrit Tube Length
Microhematocrit Tube Length
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Microhematocrit Tube Diameter
Microhematocrit Tube Diameter
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Microhematocrit Tube Volume
Microhematocrit Tube Volume
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Microhematocrit Tube Plug Length
Microhematocrit Tube Plug Length
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Hematocrit Procedure (Centrifugation)
Hematocrit Procedure (Centrifugation)
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Automated Hematocrit
Automated Hematocrit
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Hematocrit Variation
Hematocrit Variation
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Study Notes
Hematocrit Determination
- Hematocrit refers to the packed cell volume (PCV)
- After centrifuging anticoagulated whole blood, red blood cells settle at the bottom
- Packed cell volume is the volume of red blood cells
- Reference ranges:
- Adult Males: 40% to 54%
- Adult Females: 35% to 49%
- Newborns: 53% to 65%
- SI Units: 0.40 to 0.54 L/L, 0.35 to 0.49 L/L, 0.53 to 0.65 L/L respectively
- Micro-hematocrit tube:
- Approximately 75 mm long
- Inner bore: 1.2 mm
- Can hold 0.05 mL of blood
- Plug: 4 to 6 mm long
- Two types:
- Red band/ring: for non-anticoagulated blood
- Blue band/ring: for anticoagulated blood
Procedure
- Perform skin puncture
- Wipe off the first drop of blood
- Fill two heparinized capillary tubes with blood
- Seal the dry end with sealing clay and plug
- Position tubes in centrifuge radial grooves
- Spin at 10,000 RPM for 5 minutes
- Read hematocrit (exclude buffy coat layer)
Additional Notes
- Excessive anticoagulant leads to cell shrinkage
- Air bubbles in the tubes do not affect results
- Tubes are filled from opposite ends
- Results should agree within 1% ± 0.01 L/L for two samples
- Centrifuge at 10,000 g to 15,000 g to maximize red blood cell packing
- Trapped plasma can elevate the microhematocrit reading (by 1% to 3%)
- Automated hematocrit is a calculated value
- Difference in hematocrit results is usually insignificant unless poikilocytosis or anisocytosis is present
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Description
Test your knowledge on hematocrit determination, packed cell volume, and reference ranges. This quiz will cover the procedures for collecting and centrifuging blood samples effectively. Understand the differences between anticoagulated and non-anticoagulated blood testing methods.
