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Questions and Answers
What is the purpose of the osmotic fragility test?
What is the purpose of the osmotic fragility test?
What is the unit of measurement for mean cell Hb concentration (MCHC)?
What is the unit of measurement for mean cell Hb concentration (MCHC)?
What is the range of increase in RBC volume before they rupture?
What is the range of increase in RBC volume before they rupture?
What is the concentration of NaCl in the first test tube in the osmotic fragility test?
What is the concentration of NaCl in the first test tube in the osmotic fragility test?
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What is the clinical significance of increased osmotic fragility?
What is the clinical significance of increased osmotic fragility?
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What is the purpose of adding 0.1 ml of blood to each test tube in the osmotic fragility test?
What is the purpose of adding 0.1 ml of blood to each test tube in the osmotic fragility test?
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What is the formula to calculate MCHC?
What is the formula to calculate MCHC?
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What is the result of decreased osmotic fragility in iron deficiency anemia?
What is the result of decreased osmotic fragility in iron deficiency anemia?
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What is the duration of the osmotic fragility test?
What is the duration of the osmotic fragility test?
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What is the reference material for the osmotic fragility test?
What is the reference material for the osmotic fragility test?
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Study Notes
Packed Cell Volume (PCV)
- PCV is the percentage of blood volume that is made up of red blood cells
- Methods for detection: Macro hematocrit method and Micro hematocrit method
- Normal values: Male: 42-52%, Female: 37-47%
Clinical Significance of PCV
- Dehydration: increases PCV
- Polycythemia: increases PCV
- Overhydration: decreases PCV
- Anemia: decreases PCV
Complete Blood Picture (CBC)
- Calculation of cellular elements of blood
- Automated hematology analyzer determines the calculations
- Used to determine general health status, screen for, diagnose, or monitor diseases and conditions that affect blood cells
- Includes: anemia, infection, inflammation, and bleeding disorders
Red Blood Indices
- Mean Cell Volume (MCV): average volume of red blood cell measured in femtoliters (fl)
- Mean Cell Hemoglobin (MCH): average amount of Hb in a single red blood cell measured in picogram (pg)
- Mean Cell Hb Concentration (MCHC): concentration of Hb per 100 ml of RBC measured in g/dl
Erythrocyte Sedimentation Rate (ESR)
- Rate by which red cells sediment when blood is placed vertically in a narrow tube
- Westergren Apparatus: uses a calibrated tube to measure the rate of sedimentation
- Normal values: Male: 4-6 mm/h, Female: 8-10 mm/h
- Clinical significance: increases in inflammatory activity, pregnancy, menstruation, lactation, and certain diseases
Osmotic Fragility Test
- Measures erythrocyte resistance to hemolysis while exposed to varying levels of dilution of a saline solution
- Procedure: uses a series of test tubes with decreasing concentrations of NaCl
- Clinical significance: enzymatic deficiency, hereditary spherocytosis, iron deficiency anemia, thalassemia, and sickle cell anemia
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Description
This quiz covers the definition and methods of detection for hematocrit value and packed cell volume, including macro and micro hematocrit methods.
