General Biology 2 - Recombinant DNA
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Questions and Answers

The process of genetic engineering includes the identification of an organism that contains a desirable ______.

gene

A circular piece of DNA called a ______ is removed from a bacterial cell for genetic engineering.

plasmid

The inserted plasmid DNA that contains the host gene can multiply to make several ______ of the wanted gene.

copies

The process that allows a bacterial cell to acquire a gene from a different organism is known as ______ DNA technology.

<p>recombinant</p> Signup and view all the answers

Once the bacterial cells reproduce, the inserted gene is also ______ in the newly created cells.

<p>reproduced</p> Signup and view all the answers

Republic Act 8293, section _____ states that no copyright shall subsist in any work of the Government of the Philippines.

<p>176</p> Signup and view all the answers

The publisher and authors do not represent nor claim _____ over the borrowed materials.

<p>ownership</p> Signup and view all the answers

Every effort has been exerted to locate and seek _____ to use borrowed materials.

<p>permission</p> Signup and view all the answers

The _____ Team of the Module includes writers, editors, and reviewers.

<p>Development</p> Signup and view all the answers

Candelaria A. _____ served as both an editor and a reviewer in the development of the module.

<p>Mercadera</p> Signup and view all the answers

The Chief Education Supervisor for CID is _____ D. Montero.

<p>Amelinda</p> Signup and view all the answers

The _____ of Learner section in the module requires students to fill in their personal details.

<p>Name</p> Signup and view all the answers

In the title, 'Module 1: _____ DNA' refers to a key concept in genetics.

<p>Recombinant</p> Signup and view all the answers

The process of introducing a foreign piece of DNA into an organism's genome is known as ______ DNA technology.

<p>recombinant</p> Signup and view all the answers

Bacterial enzymes that cut DNA molecules at specific sequences are called ______ enzymes.

<p>restriction</p> Signup and view all the answers

The specific sites where restriction enzymes cut DNA are known as ______ sequences.

<p>nucleotide</p> Signup and view all the answers

Once introduced into bacteria, recombinant plasmids can be replicated as the ______ reproduce.

<p>bacteria</p> Signup and view all the answers

The ends of DNA fragments that can form base pairs with complementary bases are known as ______ ends.

<p>sticky</p> Signup and view all the answers

The gene that is introduced into the host organism is called the ______ gene.

<p>recombinant</p> Signup and view all the answers

After inserting the desired gene, the next step involves selecting an appropriate ______ to integrate the gene into the host.

<p>vector</p> Signup and view all the answers

The result of cloning a foreign gene in bacteria leads to a ______ colony.

<p>bacterial</p> Signup and view all the answers

The topic/purpose of the infographic was clear and concise, allowing the viewer to understand the ______.

<p>intent</p> Signup and view all the answers

Data of the infographic was accurate and relevant to the ______.

<p>topic</p> Signup and view all the answers

The infographic had a great layout, with applicable ______ creating an average layout.

<p>graphics</p> Signup and view all the answers

The color scheme enhanced the infographic and did not ______ the infographic.

<p>detract from</p> Signup and view all the answers

Citations for the infographic's sources were ______.

<p>included</p> Signup and view all the answers

The font was ______, and the color scheme enhanced the infographic.

<p>legible</p> Signup and view all the answers

The topic/purpose was somewhat broad and did not meet the ______ expectations.

<p>meets</p> Signup and view all the answers

There was an overload of ______ in the infographic.

<p>text</p> Signup and view all the answers

The shaded region represents the ______ gene site.

<p>insulin</p> Signup and view all the answers

In genetic engineering, the process often begins by cutting out ______ along dotted lines.

<p>cards</p> Signup and view all the answers

One of the key steps in genetic engineering is to compare each enzyme ______ to the base sequences.

<p>sequence</p> Signup and view all the answers

The ______ on the flow chart represents the process of genetic engineering.

<p>flow</p> Signup and view all the answers

Genetic engineering is not simply an extension of conventional plant ______.

<p>breeding</p> Signup and view all the answers

Recombinant ______ technology allows for the manipulation of DNA for various applications.

<p>DNA</p> Signup and view all the answers

Genetically modified organisms often raise concerns about their ______ to the environment.

<p>impact</p> Signup and view all the answers

Fast facts about genetically modified organisms can be found on the ______ website.

<p>University</p> Signup and view all the answers

Agrobacterium ______ is used as a vector to move genes into plant cells.

<p>tumefaciens</p> Signup and view all the answers

The most challenging issue facing genome sequencing is the inability to develop fast and accurate ______ techniques.

<p>sequencing</p> Signup and view all the answers

Genomics can be used in agriculture to improve disease resistance and generate new ______ strains.

<p>hybrid</p> Signup and view all the answers

Scientists have a general idea of where the gene will go and what it will do to the plant, but they cannot predict with ______ where an inserted gene will go on a plant chromosome.

<p>certainty</p> Signup and view all the answers

Genes can escape from genetically modified crops and jump to other ______.

<p>plants</p> Signup and view all the answers

To produce recombinant DNA, using a ______ enzyme to cut DNA and form sticky ends is essential.

<p>restriction</p> Signup and view all the answers

To produce transgenic bacteria that make insulin, scientists had to first insert the human ______ gene into a plasmid.

<p>insulin</p> Signup and view all the answers

The bacterium that contains both human DNA and bacterial DNA is now considered a/an ______.

<p>transgenic organism</p> Signup and view all the answers

Study Notes

General Biology 2 - 2nd Semester - Module 1 - Recombinant DNA

  • Module Details: This module covers recombinant DNA, genetic engineering, and genetically modified organisms (GMOs).
  • Biological Principles: The central dogma of molecular biology explains the flow of genetic information from genes to protein.
  • Activity 1: DECODE ME: This activity assesses understanding of protein synthesis and how DNA modification affects organismal characteristics. Includes identifying biomolecules like DNA, amino acids, and the process of replication.
  • Activity 2: Explain the Modification: This activity focuses on observations and analysis of rice color differences, and the concept of modification to the genetic makeup of plants (and animals) through genetic engineering.
  • Genetic Engineering: This process directly alters an organism's genome by manipulating DNA. DNA serves as a universal genetic language across all organisms.
  • Genetic Engineering Techniques: Involves identifying a desirable gene, extracting it, isolating it, preparing the target DNA, inserting the new DNA into a plasmid, reintroducing the plasmid into a cell, enabling plasmid multiplication, and then producing proteins from the inserted gene.
  • Recombinant DNA Technology: This technique introduces a foreign DNA sequence into an organism's genome. This process utilizes tools like restriction enzymes, which cut DNA at specific sequences, creating "sticky ends".
  • Transgenic Plants: Created by inserting and transferring genes to modify plant characteristics (e.g., resistance to herbicides, insects, or viruses).
  • Transgenic Animals: Used to produce specific proteins or pharmaceutical compounds while also enabling greater speed and range of selective breeding.
  • Hormones: Recombinant DNA technology allows large-scale production of hormones like insulin; the process involves inserting the relevant gene into a bacterial cell.
  • Vaccines: DNA vaccines, created through recombinant DNA technology, have revolutionized approaches to treating and preventing infectious diseases like polio, malaria, cholera, hepatitis, etc.
  • Interferon: Interferons, glycoproteins produced by cells infected by viruses, exhibit antiviral and anti-cancer properties and are produced in high quantities using recombinant DNA technology.
  • Antibiotics: Methods using recombinant DNA technology increase the production of antibiotics.
  • Commercially important Chemicals: Recombinant DNA technology aids in producing commercially important chemicals, like alcohols, organic acids, and vitamins.
  • Enzyme Engineering: This involves modifying enzyme structure, achieved by altering the gene that encodes the enzyme.
  • Disease Prevention and Treatment: Genetic engineering has revolutionized diagnosis and prevention of diseases, including producing monoclonal antibodies and applying gene therapy techniques.
  • Activity 3 & 4: In these activities, students understand the techniques surrounding desirable traits. Recombinant DNA technology and traditional breeding processes are examined, with activities like identifying desirable traits and explaining the techniques used to achieve them.
  • Activity 5: This activity deals with manipulating plasmids and using restriction enzymes, examining the process of creating recombinant DNA.
  • Assessment: This section includes comprehension questions on genetic engineering and its applications.

Additional Activities

  • Activity 8: This activity requires creating infographics about the pros and cons of genetic engineering. This section also provides a rubric for evaluation.

Appendix

  • Restriction Enzyme Sequence Cards: These provide reference sequences for restriction enzyme activity.

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Week 1 Biology 2 PDF

Description

Explore the fascinating world of recombinant DNA and genetic engineering in this module. Understand the principles of molecular biology that guide genetic modification in organisms. Engage in activities that highlight protein synthesis and the implications of genetic engineering on traits in plants and animals.

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