Fluorescence Microscopy and Applications

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What is a key feature of a confocal microscope that enhances its imaging capabilities?

Ability to eliminate out-of-focus light (correct)

Increased sensitivity to NIR photons

Use of a pinhole to increase fluorescence

Capability to image entire specimens at once

What is the primary benefit of multiphoton excitation in fluorescence microscopy?

Increased resolution in thick specimens

Elimination of background fluorescence (correct)

Enhanced sensitivity to fluorescent labels

Improved contrast in epi-fluorescence microscopy

What is the purpose of a pinhole in confocal microscopy?

To enhance the resolution of the image

To increase the intensity of the excitation light

To reduce photobleaching of fluorophores

To eliminate out-of-focus light (correct)

What is the primary application of bioimage processing?

Analysis and enhancement of biological images Signup and view all the answers

What is the main difference between epi-fluorescence and confocal microscopy?

Ability to eliminate out-of-focus light Signup and view all the answers

What is the advantage of using multiphoton excitation in combination with a confocal microscope?

No background fluorescence is produced Signup and view all the answers

What is the process by which some atoms or molecules absorb light of short wavelength and emit light of longer wavelength?

Fluorescence Signup and view all the answers

What is the advantage of fluorescence microscopy over other microscopy techniques?

Better resolution and ability to collect images in more than one colour Signup and view all the answers

What is the term for the distance between the excitation and emission peaks in a fluorophore?

Stokes shift Signup and view all the answers

What is a primary antibody in fluorescence labelling strategies?

An antibody that is directly conjugated to a fluorophore Signup and view all the answers

What is a multiphoton microscope?

A type of fluorescence microscope that uses multiple photons Signup and view all the answers

What is the purpose of gene transfer in fluorescence labelling strategies?

To introduce a gene that codes for a fluorophore Signup and view all the answers

What is the principle behind fluorescence microscopy?

Absorption of light at a specific wavelength and emission at a longer wavelength Signup and view all the answers

What is the main difference between Scanning Electron Microscopy and Transmission Electron Microscopy?

SEM uses a focused electron beam, while TEM uses a broad electron beam Signup and view all the answers

What is the purpose of labeling a sample with fluorescent dyes or proteins in fluorescence microscopy?

To specifically bind to the target molecules of interest Signup and view all the answers

What is the advantage of fluorescence microscopy in visualizing cellular and molecular structures?

Ability to visualize specific structures and processes with high sensitivity Signup and view all the answers

How does a Scanning Electron Microscope form an image?

By detecting the secondary emission of electrons from the surface of the sample Signup and view all the answers

What is the role of fluorophores in fluorescence microscopy?

To absorb light at a specific wavelength and emit light at a longer wavelength Signup and view all the answers