Fluorescence Lifetime Measurements and Pulsed Excitation

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Which property of light is the most important in bioanalysis?

Frequency

Intensity

Speed

Wavelength (correct)

What is the speed of light in a vacuum?

3 x 10^12 m/s

3 x 10^10 m/s

3 x 10^6 m/s

3 x 10^8 m/s (correct)

What happens when a molecule absorbs light?

It scatters light

It transitions from a ground state to an excited state (correct)

It reflects light

It emits light

Which of the following is a major application of fluorescence spectroscopy in biology, as mentioned in the text?

Tracking tagged molecules in complex environments Signup and view all the answers

What is a limitation of conventional fluorescence microscopes, as mentioned in the text?

Issues with taking images of thick specimens Signup and view all the answers

Which of the following is a variant of GFP mentioned in the text?

CFP Signup and view all the answers

Which of the following excitation light sources is commonly used in fluorescence microscopes?

High power light-emitting diode (LED) Signup and view all the answers

Which type of semiconductor has an excess of electrons or negative charge carriers?

n-type semiconductor Signup and view all the answers

What are the three phenomena that occur at a p-n junction?

Diffusion, formation of space charge, drift Signup and view all the answers

What is the main function of a diode?

To control the flow of electricity Signup and view all the answers

Which process occurs within nanoseconds in fluorescence lifetime measurements?

Photo saturation Signup and view all the answers

Which process is responsible for the emission of light by a substance that has absorbed light or other electromagnetic radiation?

Fluorescence Signup and view all the answers

What is the main limitation of measuring with stronger excitation light intensities in fluorescence lifetime measurements?

Photo bleaching Signup and view all the answers

What is the main difference between fluorescence and phosphorescence?

Phosphorescent materials emit light for a longer time than fluorescent materials Signup and view all the answers

What is a fluorochrome?

A chemical that emits light when exposed to UV radiation Signup and view all the answers

What is the rate of energy transfer (ket) in Förster resonance energy transfer (FRET) given by?

td Signup and view all the answers

What is the energy difference between the absorbed photon and the emitted photon called?

Stokes shift Signup and view all the answers

What is the efficiency of the energy transfer (E) in FRET experiments?

E Signup and view all the answers

According to the Beer-Lambert law, the extinction coefficient is specific to a molecular species at a given wavelength. Which of the following units is often used to express the extinction coefficient?

mol L^-1 cm^-1 Signup and view all the answers

To determine the protein contamination in a DNA solution, the absorbance at 260 nm is compared to the absorbance at 280 nm. What ratio indicates the presence of proteins?

A260/A280 < 1.8 Signup and view all the answers

What is the concentration of double stranded DNA if the absorbance at 260 nm is 0.4 and the extinction coefficient is 0.020 L mg^-1 cm^-1?

2 mg cm^-3 Signup and view all the answers

What is the concentration of single stranded DNA if the absorbance at 260 nm is 0.3 and the extinction coefficient is 0.027 L mg^-1 cm^-1?

0.03 mg cm^-3 Signup and view all the answers

Which of the following is true about forward bias in a p-n junction?

The p-type is connected with the positive terminal of the power supply and the n-type is connected with the negative terminal. Signup and view all the answers

Which of the following is true about reverse bias in a p-n junction?

The p-type is connected with the negative terminal of the power supply and the n-type is connected with the positive terminal. Signup and view all the answers

Which of the following is true about a photodiode?

It is a variant of a p-n junction. Signup and view all the answers

Which of the following is true about a photomultiplier tube (PMT)?

It multiplies the current produced by incident light by as much as 100 million times. Signup and view all the answers

Fluorescence Lifetime Measurements and Pulsed Excitation

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Questions and Answers

Which property of light is the most important in bioanalysis?

What is the speed of light in a vacuum?

What happens when a molecule absorbs light?

Which of the following is a major application of fluorescence spectroscopy in biology, as mentioned in the text?

What is a limitation of conventional fluorescence microscopes, as mentioned in the text?

Which of the following is a variant of GFP mentioned in the text?

Which of the following excitation light sources is commonly used in fluorescence microscopes?

Which type of semiconductor has an excess of electrons or negative charge carriers?

What are the three phenomena that occur at a p-n junction?

What is the main function of a diode?

Which process occurs within nanoseconds in fluorescence lifetime measurements?

Which process is responsible for the emission of light by a substance that has absorbed light or other electromagnetic radiation?

What is the main limitation of measuring with stronger excitation light intensities in fluorescence lifetime measurements?

What is the main difference between fluorescence and phosphorescence?

What is a fluorochrome?

What is the rate of energy transfer (ket) in Förster resonance energy transfer (FRET) given by?

What is the energy difference between the absorbed photon and the emitted photon called?

What is the efficiency of the energy transfer (E) in FRET experiments?

According to the Beer-Lambert law, the extinction coefficient is specific to a molecular species at a given wavelength. Which of the following units is often used to express the extinction coefficient?

To determine the protein contamination in a DNA solution, the absorbance at 260 nm is compared to the absorbance at 280 nm. What ratio indicates the presence of proteins?

What is the concentration of double stranded DNA if the absorbance at 260 nm is 0.4 and the extinction coefficient is 0.020 L mg^-1 cm^-1?

What is the concentration of single stranded DNA if the absorbance at 260 nm is 0.3 and the extinction coefficient is 0.027 L mg^-1 cm^-1?

Which of the following is true about forward bias in a p-n junction?

Which of the following is true about reverse bias in a p-n junction?

Which of the following is true about a photodiode?

Which of the following is true about a photomultiplier tube (PMT)?

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