Podcast
Questions and Answers
Which property of light is the most important in bioanalysis?
Which property of light is the most important in bioanalysis?
- Frequency
- Intensity
- Speed
- Wavelength (correct)
What is the speed of light in a vacuum?
What is the speed of light in a vacuum?
- 3 x 10^12 m/s
- 3 x 10^10 m/s
- 3 x 10^6 m/s
- 3 x 10^8 m/s (correct)
What happens when a molecule absorbs light?
What happens when a molecule absorbs light?
- It scatters light
- It transitions from a ground state to an excited state (correct)
- It reflects light
- It emits light
Which of the following is a major application of fluorescence spectroscopy in biology, as mentioned in the text?
Which of the following is a major application of fluorescence spectroscopy in biology, as mentioned in the text?
What is a limitation of conventional fluorescence microscopes, as mentioned in the text?
What is a limitation of conventional fluorescence microscopes, as mentioned in the text?
Which of the following is a variant of GFP mentioned in the text?
Which of the following is a variant of GFP mentioned in the text?
Which of the following excitation light sources is commonly used in fluorescence microscopes?
Which of the following excitation light sources is commonly used in fluorescence microscopes?
Which type of semiconductor has an excess of electrons or negative charge carriers?
Which type of semiconductor has an excess of electrons or negative charge carriers?
What are the three phenomena that occur at a p-n junction?
What are the three phenomena that occur at a p-n junction?
What is the main function of a diode?
What is the main function of a diode?
Which process occurs within nanoseconds in fluorescence lifetime measurements?
Which process occurs within nanoseconds in fluorescence lifetime measurements?
Which process is responsible for the emission of light by a substance that has absorbed light or other electromagnetic radiation?
Which process is responsible for the emission of light by a substance that has absorbed light or other electromagnetic radiation?
What is the main limitation of measuring with stronger excitation light intensities in fluorescence lifetime measurements?
What is the main limitation of measuring with stronger excitation light intensities in fluorescence lifetime measurements?
What is the main difference between fluorescence and phosphorescence?
What is the main difference between fluorescence and phosphorescence?
What is a fluorochrome?
What is a fluorochrome?
What is the rate of energy transfer (ket) in Förster resonance energy transfer (FRET) given by?
What is the rate of energy transfer (ket) in Förster resonance energy transfer (FRET) given by?
What is the energy difference between the absorbed photon and the emitted photon called?
What is the energy difference between the absorbed photon and the emitted photon called?
What is the efficiency of the energy transfer (E) in FRET experiments?
What is the efficiency of the energy transfer (E) in FRET experiments?
According to the Beer-Lambert law, the extinction coefficient is specific to a molecular species at a given wavelength. Which of the following units is often used to express the extinction coefficient?
According to the Beer-Lambert law, the extinction coefficient is specific to a molecular species at a given wavelength. Which of the following units is often used to express the extinction coefficient?
To determine the protein contamination in a DNA solution, the absorbance at 260 nm is compared to the absorbance at 280 nm. What ratio indicates the presence of proteins?
To determine the protein contamination in a DNA solution, the absorbance at 260 nm is compared to the absorbance at 280 nm. What ratio indicates the presence of proteins?
What is the concentration of double stranded DNA if the absorbance at 260 nm is 0.4 and the extinction coefficient is 0.020 L mg^-1 cm^-1?
What is the concentration of double stranded DNA if the absorbance at 260 nm is 0.4 and the extinction coefficient is 0.020 L mg^-1 cm^-1?
What is the concentration of single stranded DNA if the absorbance at 260 nm is 0.3 and the extinction coefficient is 0.027 L mg^-1 cm^-1?
What is the concentration of single stranded DNA if the absorbance at 260 nm is 0.3 and the extinction coefficient is 0.027 L mg^-1 cm^-1?
Which of the following is true about forward bias in a p-n junction?
Which of the following is true about forward bias in a p-n junction?
Which of the following is true about reverse bias in a p-n junction?
Which of the following is true about reverse bias in a p-n junction?
Which of the following is true about a photodiode?
Which of the following is true about a photodiode?
Which of the following is true about a photomultiplier tube (PMT)?
Which of the following is true about a photomultiplier tube (PMT)?
