Podcast
Questions and Answers
What is the purpose of selecting excitation and emission wavelengths in fluorescence microscopy?
What is the purpose of selecting excitation and emission wavelengths in fluorescence microscopy?
- To control the direction of fluorescence
- To control the color of fluorescence
- To control the intensity of fluorescence (correct)
- To control the duration of fluorescence
Which excitation light source is commonly used in fluorescence microscopes due to its lower energy consumption and longer lifetime compared to other light sources?
Which excitation light source is commonly used in fluorescence microscopes due to its lower energy consumption and longer lifetime compared to other light sources?
- Mercury vapor lamp
- Metal halide lamp
- High power LED (correct)
- Xenon arc lamp
What type of semiconductor is formed by doping silicon with a trivalent element like indium or boron?
What type of semiconductor is formed by doping silicon with a trivalent element like indium or boron?
- Intrinsic semiconductor
- N-type semiconductor
- Extrinsic semiconductor
- P-type semiconductor (correct)
What happens at a p-n junction in a diode?
What happens at a p-n junction in a diode?
Which of the following statements about bias in a p-n junction is correct?
Which of the following statements about bias in a p-n junction is correct?
What happens when a photon of sufficient energy strikes a photodiode?
What happens when a photon of sufficient energy strikes a photodiode?
What is the purpose of the dynodes in a photomultiplier tube (PMT)?
What is the purpose of the dynodes in a photomultiplier tube (PMT)?
What is the quantum yield of a molecule?
What is the quantum yield of a molecule?
Which of the following is true about light absorption?
Which of the following is true about light absorption?
What is the speed of light in a vacuum?
What is the speed of light in a vacuum?
Which of the following interactions of light with matter can reveal information about the sample or properties of its surface?
Which of the following interactions of light with matter can reveal information about the sample or properties of its surface?
Which wavelengths are typically used in optical methods for bioanalysis?
Which wavelengths are typically used in optical methods for bioanalysis?
Which of the following is true about absorption spectrum?
Which of the following is true about absorption spectrum?
What is the ratio A260/A280 used for?
What is the ratio A260/A280 used for?
What does the Beer-Lambert law relate?
What does the Beer-Lambert law relate?
What is the purpose of a NanoDrop instrument?
What is the purpose of a NanoDrop instrument?
Which process involves the release of energy absorbed by a substance in the form of light?
Which process involves the release of energy absorbed by a substance in the form of light?
What is the main difference between fluorescent materials and phosphorescent materials?
What is the main difference between fluorescent materials and phosphorescent materials?
What is a fluorochrome?
What is a fluorochrome?
Why does the emitted photon usually have less energy than the absorbed photon in fluorescence?
Why does the emitted photon usually have less energy than the absorbed photon in fluorescence?
Which type of quenching occurs when the fluorophore is in the excited state and collisions with other species result in relaxation of the fluorophore without photon emission?
Which type of quenching occurs when the fluorophore is in the excited state and collisions with other species result in relaxation of the fluorophore without photon emission?
What is the efficiency of energy transfer (E) in fluorescence resonance energy transfer (FRET) experiments?
What is the efficiency of energy transfer (E) in fluorescence resonance energy transfer (FRET) experiments?
What is the rate of energy transfer (ket) in the Förster equation for fluorescence resonance energy transfer (FRET)?
What is the rate of energy transfer (ket) in the Förster equation for fluorescence resonance energy transfer (FRET)?
What is the process of transferring energy from a fluorophore in the excited state to a fluorescent or non-fluorescent acceptor molecule called?
What is the process of transferring energy from a fluorophore in the excited state to a fluorescent or non-fluorescent acceptor molecule called?
Which of the following is a major application of fluorescence spectroscopy in biology?
Which of the following is a major application of fluorescence spectroscopy in biology?
What is a limitation of conventional fluorescence microscopes?
What is a limitation of conventional fluorescence microscopes?
Which of the following is a mutant of GFP that is fluorescent in a different wavelength region?
Which of the following is a mutant of GFP that is fluorescent in a different wavelength region?
What can fluorescence microscopy be used to observe in cells?
What can fluorescence microscopy be used to observe in cells?
Which type of microscope is commonly used to obtain images of individual cells?
Which type of microscope is commonly used to obtain images of individual cells?
In what type of culture are mammalian cells typically grown in the lab?
In what type of culture are mammalian cells typically grown in the lab?
Which scientist first coined the term 'plasmid' in 1952?
Which scientist first coined the term 'plasmid' in 1952?
What is a plasmid?
What is a plasmid?
What is the normal number of copies of plasmid found in a single cell called?
What is the normal number of copies of plasmid found in a single cell called?
What is the purpose of CsCl density gradient centrifugation in isolating plasmids?
What is the purpose of CsCl density gradient centrifugation in isolating plasmids?
Which of the following is a key factor that limits the number of DNA molecules that can be achieved in a PCR reaction?
Which of the following is a key factor that limits the number of DNA molecules that can be achieved in a PCR reaction?
What is the purpose of real-time PCR?
What is the purpose of real-time PCR?
Which detection scheme is commonly used in PCR reactions to track the progress of the reaction?
Which detection scheme is commonly used in PCR reactions to track the progress of the reaction?
What is the efficiency of a PCR reaction represented by?
What is the efficiency of a PCR reaction represented by?
Which enzyme is added to the pyrosequencing reaction mixture to catalyze the reaction of pyrophosphate (PPi) with adenosine 5’ phosphosulfate (APS)?
Which enzyme is added to the pyrosequencing reaction mixture to catalyze the reaction of pyrophosphate (PPi) with adenosine 5’ phosphosulfate (APS)?
What triggers the release of visible light in pyrosequencing?
What triggers the release of visible light in pyrosequencing?
What is the limitation of pyrosequencing when it comes to detecting homopolymeric regions?
What is the limitation of pyrosequencing when it comes to detecting homopolymeric regions?
What is the purpose of using deoxyadenosine α-thio triphosphate (dATP-αS) instead of natural dATP in pyrosequencing?
What is the purpose of using deoxyadenosine α-thio triphosphate (dATP-αS) instead of natural dATP in pyrosequencing?
Which of the following is true about Ct values in real-time PCR?
Which of the following is true about Ct values in real-time PCR?
What is the formula used to calculate fold changes in gene expression in qRT-PCR?
What is the formula used to calculate fold changes in gene expression in qRT-PCR?
What is the purpose of relative quantification in gene expression analysis?
What is the purpose of relative quantification in gene expression analysis?
What is a disadvantage of qPCR compared to other methods?
What is a disadvantage of qPCR compared to other methods?
Which detection method in real-time PCR involves the use of two sequence-specific oligonucleotide probes labeled with different dyes?
Which detection method in real-time PCR involves the use of two sequence-specific oligonucleotide probes labeled with different dyes?
What is the purpose of melting curve analysis in real-time PCR?
What is the purpose of melting curve analysis in real-time PCR?
What is reverse transcription?
What is reverse transcription?
Which type of virus uses RNA as its genetic material and replicates through reverse transcription?
Which type of virus uses RNA as its genetic material and replicates through reverse transcription?
Which of the following methods can be used to isolate DNA and RNA from living cells?
Which of the following methods can be used to isolate DNA and RNA from living cells?
What is the purpose of centrifuging a sample mixed with cesium chloride (CsCl) at very high speeds in an ultracentrifuge?
What is the purpose of centrifuging a sample mixed with cesium chloride (CsCl) at very high speeds in an ultracentrifuge?
Which of the following is true about the separation of DNA, RNA, and protein using CsCl centrifugation?
Which of the following is true about the separation of DNA, RNA, and protein using CsCl centrifugation?
How can the separated DNA be removed from the centrifugation tube?
How can the separated DNA be removed from the centrifugation tube?
Which of the following is a common method used to select specifically for mRNAs in RNA-seq experiments?
Which of the following is a common method used to select specifically for mRNAs in RNA-seq experiments?
What is the purpose of second strand synthesis in RNA-seq experiments?
What is the purpose of second strand synthesis in RNA-seq experiments?
What is the role of ribonuclease H (RNase H) in RNA-seq experiments?
What is the role of ribonuclease H (RNase H) in RNA-seq experiments?
Why might random hexamers be used instead of oligo(dT) primers for first strand cDNA synthesis in RNA-seq experiments?
Why might random hexamers be used instead of oligo(dT) primers for first strand cDNA synthesis in RNA-seq experiments?
What is the purpose of adapter ligation in RNA-seq library construction?
What is the purpose of adapter ligation in RNA-seq library construction?
Why are ribosomal RNAs (rRNAs) often depleted from the total RNA pool in RNA-seq experiments?
Why are ribosomal RNAs (rRNAs) often depleted from the total RNA pool in RNA-seq experiments?
Which enzyme is added to the pyrosequencing reaction mixture to catalyze the reaction of pyrophosphate (PPi) with adenosine 5’ phosphosulfate (APS)?
Which enzyme is added to the pyrosequencing reaction mixture to catalyze the reaction of pyrophosphate (PPi) with adenosine 5’ phosphosulfate (APS)?
What triggers the release of visible light in pyrosequencing?
What triggers the release of visible light in pyrosequencing?
What is the current market share of the Asian market in the global NGS market as of 2017?
What is the current market share of the Asian market in the global NGS market as of 2017?
Which sequencing technology uses reversible terminators?
Which sequencing technology uses reversible terminators?
Which of the following statements about plasmids is true?
Which of the following statements about plasmids is true?
What is the purpose of CsCl density gradient centrifugation in isolating plasmids?
What is the purpose of CsCl density gradient centrifugation in isolating plasmids?
What is the purpose of phenol-chloroform extraction in DNA or RNA purification?
What is the purpose of phenol-chloroform extraction in DNA or RNA purification?
What is the purpose of minipreparation of plasmid DNA?
What is the purpose of minipreparation of plasmid DNA?
Which detection method in real-time PCR involves the use of a dye that intercalates with double stranded DNA and causes fluorescence?
Which detection method in real-time PCR involves the use of a dye that intercalates with double stranded DNA and causes fluorescence?
Which detection method in real-time PCR involves the use of a probe that is cleaved by the polymerase enzyme, resulting in increased fluorescence?
Which detection method in real-time PCR involves the use of a probe that is cleaved by the polymerase enzyme, resulting in increased fluorescence?
Which detection method in real-time PCR involves the use of two sequence-specific oligonucleotide probes labeled with different dyes?
Which detection method in real-time PCR involves the use of two sequence-specific oligonucleotide probes labeled with different dyes?
What is the purpose of melting curve analysis in real-time PCR?
What is the purpose of melting curve analysis in real-time PCR?
Which of the following is a key factor that limits the number of DNA molecules that can be achieved in a PCR reaction?
Which of the following is a key factor that limits the number of DNA molecules that can be achieved in a PCR reaction?
What is the formula used to calculate the theoretically predicted number of DNA molecules, Nm, at the end of a PCR reaction?
What is the formula used to calculate the theoretically predicted number of DNA molecules, Nm, at the end of a PCR reaction?
What is the purpose of real-time PCR?
What is the purpose of real-time PCR?
What are the two major detection schemes commonly used to track the progress of a PCR reaction?
What are the two major detection schemes commonly used to track the progress of a PCR reaction?
Which of the following is a method used to isolate DNA and RNA from living cells?
Which of the following is a method used to isolate DNA and RNA from living cells?
What is the purpose of CsCl density gradient centrifugation in isolating plasmids?
What is the purpose of CsCl density gradient centrifugation in isolating plasmids?
What triggers the release of visible light in pyrosequencing?
What triggers the release of visible light in pyrosequencing?
What is the purpose of adapter ligation in RNA-seq library construction?
What is the purpose of adapter ligation in RNA-seq library construction?
Which of the following is true about Ct values in real-time PCR?
Which of the following is true about Ct values in real-time PCR?
What is the purpose of a standard curve in absolute quantification of gene expression?
What is the purpose of a standard curve in absolute quantification of gene expression?
What is the purpose of relative quantification in gene expression analysis?
What is the purpose of relative quantification in gene expression analysis?
What is the purpose of the cleavable hairpin beacon (CHB) in colorimetric LAMP?
What is the purpose of the cleavable hairpin beacon (CHB) in colorimetric LAMP?
Which of the following is a key application of RNA-seq?
Which of the following is a key application of RNA-seq?
What is the purpose of depleting ribosomal RNAs (rRNAs) in RNA-seq experiments?
What is the purpose of depleting ribosomal RNAs (rRNAs) in RNA-seq experiments?
What is the main advantage of using oligo(dT) beads for RNA-seq?
What is the main advantage of using oligo(dT) beads for RNA-seq?
What is the purpose of using random hexamers for first strand cDNA synthesis in RNA-seq?
What is the purpose of using random hexamers for first strand cDNA synthesis in RNA-seq?
What is the purpose of adding an 'A' base to the 3' ends of dsDNAs in RNA-seq library construction?
What is the purpose of adding an 'A' base to the 3' ends of dsDNAs in RNA-seq library construction?
What is the purpose of Y-shaped adapters in Illumina library preparation for RNA-seq?
What is the purpose of Y-shaped adapters in Illumina library preparation for RNA-seq?