Podcast
Questions and Answers
What is the primary function of appropriate filters in fluorescence microscopy?
What is the primary function of appropriate filters in fluorescence microscopy?
- To control the intensity of the emission light
- To enhance the fluorescence intensity
- To control the intensity of the excitation light
- To select the excitation and emission wavelengths (correct)
Which of the following excitation light sources is commonly used in fluorescence microscopes?
Which of the following excitation light sources is commonly used in fluorescence microscopes?
- Metal halide lamp
- High power LED (correct)
- Mercury vapor lamp
- Xenon arc lamp
What type of semiconductor is formed by doping silicon with a trivalent element?
What type of semiconductor is formed by doping silicon with a trivalent element?
- Intrinsic semiconductor
- N-type semiconductor
- Extrinsic semiconductor
- P-type semiconductor (correct)
What occurs at a p-n junction in a semiconductor?
What occurs at a p-n junction in a semiconductor?
Which process releases energy relatively slowly in the form of light?
Which process releases energy relatively slowly in the form of light?
What happens to fluorescence when the radiation source stops?
What happens to fluorescence when the radiation source stops?
What is a fluorochrome?
What is a fluorochrome?
Why does the emission spectrum have longer wavelengths than the excitation spectrum?
Why does the emission spectrum have longer wavelengths than the excitation spectrum?
Which property of light is the most important in bioanalysis?
Which property of light is the most important in bioanalysis?
What is the speed of light in a vacuum?
What is the speed of light in a vacuum?
What are the possible interactions when light is exposed to a material?
What are the possible interactions when light is exposed to a material?
What does the absorption of light result in?
What does the absorption of light result in?
Which of the following is true about forward bias in a p-n junction?
Which of the following is true about forward bias in a p-n junction?
Which of the following is true about reverse bias in a p-n junction?
Which of the following is true about reverse bias in a p-n junction?
What happens when a photon of sufficient energy strikes a photodiode?
What happens when a photon of sufficient energy strikes a photodiode?
What is the quantum yield of a molecule?
What is the quantum yield of a molecule?
Which of the following is a limitation of using stronger excitation light intensities in fluorescence lifetime measurements?
Which of the following is a limitation of using stronger excitation light intensities in fluorescence lifetime measurements?
Which type of quenching occurs when the fluorophore is in the excited state and collisions with other species result in relaxation of the fluorophore without photon emission?
Which type of quenching occurs when the fluorophore is in the excited state and collisions with other species result in relaxation of the fluorophore without photon emission?
What is the efficiency of energy transfer in fluorescence resonance energy transfer (FRET) experiments?
What is the efficiency of energy transfer in fluorescence resonance energy transfer (FRET) experiments?
What is the Förster distance in the Förster equation for calculating the rate of energy transfer in FRET?
What is the Förster distance in the Förster equation for calculating the rate of energy transfer in FRET?
According to the Beer-Lambert law, the linear relation between concentration and absorbance is described by the equation $A = \epsilon \cdot c \cdot l$, where $A$ is the absorbance, $\epsilon$ is the extinction coefficient, $c$ is the concentration, and $l$ is the path length. Which of the following statements is true about this equation?
According to the Beer-Lambert law, the linear relation between concentration and absorbance is described by the equation $A = \epsilon \cdot c \cdot l$, where $A$ is the absorbance, $\epsilon$ is the extinction coefficient, $c$ is the concentration, and $l$ is the path length. Which of the following statements is true about this equation?
Which of the following statements is true about the NanoDrop spectrophotometer?
Which of the following statements is true about the NanoDrop spectrophotometer?
What is the purpose of measuring the absorbance of the sample-free solvent ("blank") in spectrophotometry?
What is the purpose of measuring the absorbance of the sample-free solvent ("blank") in spectrophotometry?
What is the typical absorbance ratio A260/A280 for a pure DNA solution?
What is the typical absorbance ratio A260/A280 for a pure DNA solution?
Which of the following is a major application of fluorescence spectroscopy in biology?
Which of the following is a major application of fluorescence spectroscopy in biology?
What is a limitation of conventional fluorescence microscopes?
What is a limitation of conventional fluorescence microscopes?
Which of the following is a mutant of GFP that is fluorescent in a different wavelength region?
Which of the following is a mutant of GFP that is fluorescent in a different wavelength region?
What can be observed using fluorescence microscopy?
What can be observed using fluorescence microscopy?
What are the major applications of fluorescence spectroscopy in biology?
What are the major applications of fluorescence spectroscopy in biology?
What are the limitations of conventional fluorescence microscopes?
What are the limitations of conventional fluorescence microscopes?
Which process releases energy relatively slowly in the form of light?
Which process releases energy relatively slowly in the form of light?
What is a fluorochrome?
What is a fluorochrome?
Why is there an increase in wavelength?
Why is there an increase in wavelength?
What is the purpose of measuring the absorbance of the sample-free solvent ('blank') in spectrophotometry?
What is the purpose of measuring the absorbance of the sample-free solvent ('blank') in spectrophotometry?
Which of the following is true about the operation of a photodiode?
Which of the following is true about the operation of a photodiode?
What is the purpose of the dynodes in a photomultiplier tube (PMT)?
What is the purpose of the dynodes in a photomultiplier tube (PMT)?
What is the absorption maximum of fluorescein?
What is the absorption maximum of fluorescein?
What is the quantum yield of fluorescein in 0.1 M NaOH?
What is the quantum yield of fluorescein in 0.1 M NaOH?
Which of the following excitation light sources is commonly used in fluorescence microscopes?
Which of the following excitation light sources is commonly used in fluorescence microscopes?
What is the primary function of appropriate filters in fluorescence microscopy?
What is the primary function of appropriate filters in fluorescence microscopy?
What occurs at a p-n junction in a semiconductor?
What occurs at a p-n junction in a semiconductor?
What is a diode?
What is a diode?
Which of the following interactions can occur when light is exposed to a material?
Which of the following interactions can occur when light is exposed to a material?
What is the speed of light in a vacuum?
What is the speed of light in a vacuum?
What property of light is the most important in bioanalysis?
What property of light is the most important in bioanalysis?
According to the Beer-Lambert law, the linear relation between concentration and absorbance is described by the equation $A = \epsilon \cdot c \cdot l$, where $A$ is the absorbance, $\epsilon$ is the extinction coefficient, $c$ is the concentration, and $l$ is the path length. Which of the following statements is true about this equation?
According to the Beer-Lambert law, the linear relation between concentration and absorbance is described by the equation $A = \epsilon \cdot c \cdot l$, where $A$ is the absorbance, $\epsilon$ is the extinction coefficient, $c$ is the concentration, and $l$ is the path length. Which of the following statements is true about this equation?
According to the Beer-Lambert law, the linear relation between concentration and absorbance is described by the equation $A = \epsilon \cdot c \cdot l$, where $A$ is the absorbance, $\epsilon$ is the extinction coefficient, $c$ is the concentration, and $l$ is the path length. Which of the following statements is true about this equation?
According to the Beer-Lambert law, the linear relation between concentration and absorbance is described by the equation $A = \epsilon \cdot c \cdot l$, where $A$ is the absorbance, $\epsilon$ is the extinction coefficient, $c$ is the concentration, and $l$ is the path length. Which of the following statements is true about this equation?
What is the typical absorbance ratio A260/A280 for a pure DNA solution?
What is the typical absorbance ratio A260/A280 for a pure DNA solution?
Which of the following is true about the NanoDrop spectrophotometer?
Which of the following is true about the NanoDrop spectrophotometer?
What is the efficiency of energy transfer in fluorescence resonance energy transfer (FRET) experiments?
What is the efficiency of energy transfer in fluorescence resonance energy transfer (FRET) experiments?
Which of the following is true about fluorescence lifetime measurements?
Which of the following is true about fluorescence lifetime measurements?
What is the main limitation of using stronger excitation light intensities in fluorescence lifetime measurements?
What is the main limitation of using stronger excitation light intensities in fluorescence lifetime measurements?
Which type of quenching occurs when a fluorophore is in the excited state and collisions with other species result in relaxation of the fluorophore without photon emission?
Which type of quenching occurs when a fluorophore is in the excited state and collisions with other species result in relaxation of the fluorophore without photon emission?
What is fluorescence resonance energy transfer (FRET)?
What is fluorescence resonance energy transfer (FRET)?
Which of the following is a major application of fluorescence spectroscopy in biology?
Which of the following is a major application of fluorescence spectroscopy in biology?
What is the primary function of appropriate filters in fluorescence microscopy?
What is the primary function of appropriate filters in fluorescence microscopy?
What is a limitation of conventional fluorescence microscopes?
What is a limitation of conventional fluorescence microscopes?
What are the possible interactions when light is exposed to a material?
What are the possible interactions when light is exposed to a material?
What is the efficiency of energy transfer in fluorescence resonance energy transfer (FRET) experiments?
What is the efficiency of energy transfer in fluorescence resonance energy transfer (FRET) experiments?
Which of the following is a mutant of GFP that is fluorescent in a different wavelength region?
Which of the following is a mutant of GFP that is fluorescent in a different wavelength region?
