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Questions and Answers
What is the main purpose of Fluorescence In Situ Hybridization (FISH)?
What is the main purpose of Fluorescence In Situ Hybridization (FISH)?
- Identifying specific chromosomal regions (correct)
- Analyzing cellular protein expression
- Detecting protein, RNA, and DNA structures in situ
- Visualizing cell growth in culture
How is the specificity of FISH probes achieved?
How is the specificity of FISH probes achieved?
- Probes are randomly distributed throughout the genome
- Probes are designed for specific chromosomes or chromosomal regions (correct)
- Probes are designed to target RNA sequences
- Probes are designed to target all DNA sequences in the nucleus
What does the presence of two signals per nucleus indicate in a FISH analysis with a probe to chromosome 22?
What does the presence of two signals per nucleus indicate in a FISH analysis with a probe to chromosome 22?
- Duplication in chromosome 22
- Translocation involving chromosome 22
- Deletion in chromosome 22
- Normal chromosome 22 (correct)
In FISH, what does the detection of one signal per nucleus with a specific probe indicate?
In FISH, what does the detection of one signal per nucleus with a specific probe indicate?
What is the advantage of using Fluorescence In Situ Hybridization (FISH) in cytogenetic analysis?
What is the advantage of using Fluorescence In Situ Hybridization (FISH) in cytogenetic analysis?
What is the size range of the DNA fragment probe used in Interphase FISH?
What is the size range of the DNA fragment probe used in Interphase FISH?
What does the detection of more than two signals per nucleus with a specific probe indicate in FISH analysis?
What does the detection of more than two signals per nucleus with a specific probe indicate in FISH analysis?
In which situations is Fluorescence In Situ Hybridization (FISH) commonly used?
In which situations is Fluorescence In Situ Hybridization (FISH) commonly used?
What is the main advantage of using Fluorescence In Situ Hybridization (FISH) in cytogenetic analysis?
What is the main advantage of using Fluorescence In Situ Hybridization (FISH) in cytogenetic analysis?
What is the purpose of using multiple probes in FISH analysis?
What is the purpose of using multiple probes in FISH analysis?
What is the purpose of FISH technique?
What is the purpose of FISH technique?
What is the size range of the DNA fragment probe used in FISH?
What is the size range of the DNA fragment probe used in FISH?
How is the probe specificity achieved in FISH?
How is the probe specificity achieved in FISH?
What does the presence of one signal from a FISH probe indicate?
What does the presence of one signal from a FISH probe indicate?
In which situations is FISH commonly used?
In which situations is FISH commonly used?
Flashcards
FISH Main Purpose
FISH Main Purpose
To identify specific chromosomal regions within a cell.
FISH Probe Specificity
FISH Probe Specificity
Probes are designed to target and bind to specific DNA sequences on particular chromosomes or regions.
Two FISH Signals
Two FISH Signals
Indicates a normal chromosome 22, as each cell typically has two copies of each chromosome.
One FISH Signal
One FISH Signal
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FISH Advantage
FISH Advantage
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Interphase FISH Probe Size
Interphase FISH Probe Size
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Multiple FISH Signals
Multiple FISH Signals
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FISH Usage
FISH Usage
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FISH Multiple Probes
FISH Multiple Probes
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FISH Technique Purpose
FISH Technique Purpose
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Study Notes
Fluorescence In Situ Hybridization (FISH)
- Main purpose: To detect and locate specific DNA sequences on chromosomes.
Specificity of FISH Probes
- Achieved through carefully designing probes to be complementary to specific DNA sequences.
Interpretation of FISH Results
- Presence of two signals per nucleus: Indicates two copies of the specific chromosome (e.g., chromosome 22) per cell.
- Detection of one signal per nucleus: Indicates a deletion or loss of the specific chromosome.
- Detection of more than two signals per nucleus: Indicates amplification or gain of the specific chromosome.
Advantages of FISH
- Allows for rapid detection of chromosomal abnormalities in interphase cells.
- Enables precise localization of specific DNA sequences on chromosomes.
Probe Characteristics
- Size range of DNA fragment probes in Interphase FISH: 50-500 kb.
- Size range of DNA fragment probes in FISH: 50-1000 kb.
- Probe specificity is achieved through careful design to be complementary to specific DNA sequences.
Common Applications of FISH
- Detection of chromosomal abnormalities, such as deletions, amplifications, and translocations.
- Cancer diagnosis and monitoring.
- Prenatal diagnosis of chromosomal abnormalities.
- Detection of genetic disorders.
Multiple Probes in FISH
- Purpose: To detect multiple chromosomes or genetic loci simultaneously.
Purpose of FISH Technique
- To visualize specific DNA sequences on chromosomes in intact cells.
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