15 Questions
What is the main purpose of Fluorescence In Situ Hybridization (FISH)?
Identifying specific chromosomal regions
How is the specificity of FISH probes achieved?
Probes are designed for specific chromosomes or chromosomal regions
What does the presence of two signals per nucleus indicate in a FISH analysis with a probe to chromosome 22?
Normal chromosome 22
In FISH, what does the detection of one signal per nucleus with a specific probe indicate?
Deletion in the targeted chromosomal region
What is the advantage of using Fluorescence In Situ Hybridization (FISH) in cytogenetic analysis?
Growth of cells in culture not required
What is the size range of the DNA fragment probe used in Interphase FISH?
60-200 kb
What does the detection of more than two signals per nucleus with a specific probe indicate in FISH analysis?
Duplication
In which situations is Fluorescence In Situ Hybridization (FISH) commonly used?
Prenatal samples, tumors, and hematological malignancies
What is the main advantage of using Fluorescence In Situ Hybridization (FISH) in cytogenetic analysis?
Growth of cells in culture not required
What is the purpose of using multiple probes in FISH analysis?
To detect regional deletions
What is the purpose of FISH technique?
Detecting protein, RNA, and DNA structures in situ
What is the size range of the DNA fragment probe used in FISH?
60-200 kb
How is the probe specificity achieved in FISH?
Probes designed for specific chromosomes or chromosomal regions
What does the presence of one signal from a FISH probe indicate?
Deletion
In which situations is FISH commonly used?
Prenatal samples, tumors, and hematological malignancies
Study Notes
Fluorescence In Situ Hybridization (FISH)
- Main purpose: To detect and locate specific DNA sequences on chromosomes.
Specificity of FISH Probes
- Achieved through carefully designing probes to be complementary to specific DNA sequences.
Interpretation of FISH Results
- Presence of two signals per nucleus: Indicates two copies of the specific chromosome (e.g., chromosome 22) per cell.
- Detection of one signal per nucleus: Indicates a deletion or loss of the specific chromosome.
- Detection of more than two signals per nucleus: Indicates amplification or gain of the specific chromosome.
Advantages of FISH
- Allows for rapid detection of chromosomal abnormalities in interphase cells.
- Enables precise localization of specific DNA sequences on chromosomes.
Probe Characteristics
- Size range of DNA fragment probes in Interphase FISH: 50-500 kb.
- Size range of DNA fragment probes in FISH: 50-1000 kb.
- Probe specificity is achieved through careful design to be complementary to specific DNA sequences.
Common Applications of FISH
- Detection of chromosomal abnormalities, such as deletions, amplifications, and translocations.
- Cancer diagnosis and monitoring.
- Prenatal diagnosis of chromosomal abnormalities.
- Detection of genetic disorders.
Multiple Probes in FISH
- Purpose: To detect multiple chromosomes or genetic loci simultaneously.
Purpose of FISH Technique
- To visualize specific DNA sequences on chromosomes in intact cells.
Test your knowledge of Fluorescence In Situ Hybridization (FISH) with this quiz. Explore the principles and applications of FISH, including its use in detecting protein, RNA, and DNA structures in cytogenetic analysis. Brush up on the details of DNA probe hybridization and visualize the process under a fluorescent microscope.
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