Electrophoresis: Principles and Terminologies

Electrophoresis: Principles and Terminologies

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Questions and Answers

Agarose Gel is a widely used supporting media that requires a small amount of sample. Another type of support material commonly used is ________ Gel.

Polyacrylamide

Polyacrylamide Gel involves the separation of proteins based on ________ and molecular size.

charge

Starch Gel is involved in the separation of proteins similar to polyacrylamide gel, but it is not widely used due to technical difficulty in preparing the ________.

gel

Horizontal Electrophoresis Gel Apparatus has limited gel thickness and can accommodate only ________ gel per apparatus.

<p>one</p> Signup and view all the answers

Vertical Electrophoresis Gel Apparatus allows the use of different gel thicknesses and can accommodate more than ________ gel per apparatus.

<p>one</p> Signup and view all the answers

In Paper Electrophoresis, nucleic acids consistently migrate towards the ________ due to their negative charge imparted by the phosphate backbone.

<p>anode</p> Signup and view all the answers

Electrophoresis is a separation technique based on the differential migration features of charged molecules in an ______ field.

<p>electric</p> Signup and view all the answers

Iontophoresis involves the migration of small ______.

<p>ions</p> Signup and view all the answers

Zone Electrophoresis refers to the migration of charged macromolecules in a porous support ______.

<p>medium</p> Signup and view all the answers

Electrophoretogram is the result of zone electrophoresis and consists of sharply separated zones of ______.

<p>macromolecule</p> Signup and view all the answers

Charged particles in electrophoresis migrate toward the opposite charged ______.

<p>electrode</p> Signup and view all the answers

Gel Electrophoresis uses a gelatinous material as a support ______.

<p>medium</p> Signup and view all the answers

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