V10_Capillary Isoelectric Focusing (CIEF) in Electrophoresis

Capillary Isoelectric Focusing (CIEF) separates molecules based on their charge-to-size ratio only.

In Capillary Isoelectric Focusing, the net charge of a molecule is zero at the isoelectric point (IEP).

The separation of charged molecules in Capillary Isoelectric Focusing is solely based on their molecular weight.

Capillary Isoelectric Focusing allows for the collection and concentration of particles with different charges.

Charged molecules stop moving when they approach their isoelectric point in Isoelectric Focusing.

Net charge of a molecule in Capillary Isoelectric Focusing is not affected by the pH value of the solute.

EOF must be high to ensure efficient separation in Capillary Isoelectric Focusing (CIEF)

Capillary Electrophoresis requires low voltages for separation and detection

Capillary Electrophoresis is suitable for continuous monitoring due to its efficiency

Capillary Isoelectric Focusing is not applicable for insoluble molecules due to its mechanism

The use of sensitive detectors is not a characteristic of Capillary Electrophoresis systems

Automation is not a feature of Capillary Electrophoresis systems

Ampholytes are molecules that contain ionizable acidic and alkaline groups depending on the pH of the molecule's environment.

In Capillary Isoelectric Focusing (CIEF), a stable pH gradient is formed inside the capillary by amphiphiles.

The IEP stands for Isoelectric Enzyme Point in Capillary Isoelectric Focusing.

In Capillary Isoelectric Focusing, a low pH electrolyte (anolyte) is connected to the end of the capillary where electric field is applied.

Capillary Electrophoresis on a Chip and Capillary Isoelectric Focusing (cIEF) are two completely different techniques with no similarities.

Ampholytes become neutral when the positive and negative charges in the molecule are balanced at their Intermediate Electron Potential (IEP).