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Questions and Answers
Capillary Isoelectric Focusing (CIEF) separates molecules based on their charge-to-size ratio only.
Capillary Isoelectric Focusing (CIEF) separates molecules based on their charge-to-size ratio only.
False
In Capillary Isoelectric Focusing, the net charge of a molecule is zero at the isoelectric point (IEP).
In Capillary Isoelectric Focusing, the net charge of a molecule is zero at the isoelectric point (IEP).
True
The separation of charged molecules in Capillary Isoelectric Focusing is solely based on their molecular weight.
The separation of charged molecules in Capillary Isoelectric Focusing is solely based on their molecular weight.
False
Capillary Isoelectric Focusing allows for the collection and concentration of particles with different charges.
Capillary Isoelectric Focusing allows for the collection and concentration of particles with different charges.
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Charged molecules stop moving when they approach their isoelectric point in Isoelectric Focusing.
Charged molecules stop moving when they approach their isoelectric point in Isoelectric Focusing.
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Net charge of a molecule in Capillary Isoelectric Focusing is not affected by the pH value of the solute.
Net charge of a molecule in Capillary Isoelectric Focusing is not affected by the pH value of the solute.
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EOF must be high to ensure efficient separation in Capillary Isoelectric Focusing (CIEF)
EOF must be high to ensure efficient separation in Capillary Isoelectric Focusing (CIEF)
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Capillary Electrophoresis requires low voltages for separation and detection
Capillary Electrophoresis requires low voltages for separation and detection
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Capillary Electrophoresis is suitable for continuous monitoring due to its efficiency
Capillary Electrophoresis is suitable for continuous monitoring due to its efficiency
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Capillary Isoelectric Focusing is not applicable for insoluble molecules due to its mechanism
Capillary Isoelectric Focusing is not applicable for insoluble molecules due to its mechanism
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The use of sensitive detectors is not a characteristic of Capillary Electrophoresis systems
The use of sensitive detectors is not a characteristic of Capillary Electrophoresis systems
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Automation is not a feature of Capillary Electrophoresis systems
Automation is not a feature of Capillary Electrophoresis systems
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Ampholytes are molecules that contain ionizable acidic and alkaline groups depending on the pH of the molecule's environment.
Ampholytes are molecules that contain ionizable acidic and alkaline groups depending on the pH of the molecule's environment.
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In Capillary Isoelectric Focusing (CIEF), a stable pH gradient is formed inside the capillary by amphiphiles.
In Capillary Isoelectric Focusing (CIEF), a stable pH gradient is formed inside the capillary by amphiphiles.
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The IEP stands for Isoelectric Enzyme Point in Capillary Isoelectric Focusing.
The IEP stands for Isoelectric Enzyme Point in Capillary Isoelectric Focusing.
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In Capillary Isoelectric Focusing, a low pH electrolyte (anolyte) is connected to the end of the capillary where electric field is applied.
In Capillary Isoelectric Focusing, a low pH electrolyte (anolyte) is connected to the end of the capillary where electric field is applied.
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Capillary Electrophoresis on a Chip and Capillary Isoelectric Focusing (cIEF) are two completely different techniques with no similarities.
Capillary Electrophoresis on a Chip and Capillary Isoelectric Focusing (cIEF) are two completely different techniques with no similarities.
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Ampholytes become neutral when the positive and negative charges in the molecule are balanced at their Intermediate Electron Potential (IEP).
Ampholytes become neutral when the positive and negative charges in the molecule are balanced at their Intermediate Electron Potential (IEP).
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