Electrophoresis Principles and Applications
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Questions and Answers

What is the main basis for the separation of proteins in SDS-PAGE?

  • Composition
  • Charge
  • Size (correct)
  • Shape

    • What is the purpose of using sodium dodecyl sulfate (SDS) in SDS-PAGE?

  • To separate proteins based on size
  • To neutralize the charge of proteins (correct)
  • To bind with proteins based on composition
  • To alter the shape of proteins

    • Why do smaller polypeptides move faster in SDS-PAGE?

  • They have a different shape
  • They have a different composition
  • They have a different charge
  • They have less hindrance (correct)

    • Which factor does not significantly impact the separation of proteins in SDS-PAGE?

    <p>Shape</p> Signup and view all the answers

    What property of proteins primarily affects their electrophoretic mobility in SDS-PAGE?

    <p>Size</p> Signup and view all the answers

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