Electrophoresis Principles and Applications

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What is the main basis for the separation of proteins in SDS-PAGE?

Size

What is the purpose of using sodium dodecyl sulfate (SDS) in SDS-PAGE?

To neutralize the charge of proteins

Why do smaller polypeptides move faster in SDS-PAGE?

They have less hindrance

Which factor does not significantly impact the separation of proteins in SDS-PAGE?

Shape

What property of proteins primarily affects their electrophoretic mobility in SDS-PAGE?

Size

Test your knowledge of electrophoresis, a technique based on the mobility of ions in an electric field used for separating mixtures of amino acids or DNA fragments. Learn about SDS-PAGE and its role in separating proteins based on molecular weight.

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