Effects of Karonudib on AXL-/- and MTH1-/- Cell Lines

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What is the projected number of new myeloma cases per year in the UK by 2038-2040?

6000

8300 (correct)

7000

9500

What is the main objective of the research in determining the role of MTH1 and AXL in Karonudib sensitivity?

To compare the efficacy of TH1579 and Karonudib in treating cutaneous malignant melanoma

To develop a new mouse model for myeloma

To study the effects of Palbociclib on myeloma cells

To identify whether MTH1 and AXL contribute to Karonudib induction of ROS and apoptosis (correct)

What is the main function of MTH1 in cancer cells?

To induce apoptosis in cancer cells

To promote the growth of cancer cells

To remove oxidized nucleotide from dNTP pool and prevent oxidative DNA damage (correct)

To inhibit the action of Karonudib

What is the purpose of the Myeloma-Osteoblast co-culture methodology?

To induce dormancy in myeloma cells Signup and view all the answers

What is the effect of inhibiting MTH1 in cancer cells?

It induces apoptosis in cancer cells Signup and view all the answers

What is the target of Karonudib in cancer cells?

MTH1 Signup and view all the answers

What is the function of CellROX Green Flow assay in the research?

To measure ROS production Signup and view all the answers

What is the purpose of including Palbociclib in the research?

To study the effects of CDK4/6 inhibition on myeloma cells Signup and view all the answers

What is the role of AXL in cancer cells?

It is involved in cancer dormancy Signup and view all the answers

What is the name of the disease being studied in this research?

Multiple Myeloma Signup and view all the answers

What is the purpose of the Resazurin assay in the research?

To determine cell viability in response to Karonudib Signup and view all the answers

What is the expected outcome of the research in MTH1-/- cells?

Resistance to Karonudib Signup and view all the answers

What is the purpose of checking the effect of drug Karonudib in dormant AXL-/- and MTH1-/- cell lines?

To investigate the anti-tumor effects of Karonudib in cancer cells Signup and view all the answers

What is the role of osteoclasts in myeloma cells?

They control the reactivation of dormant myeloma cells Signup and view all the answers

What is the consequence of MTH1 inhibition in cancer cells?

It induces DNA strand breaks and genotoxic damage Signup and view all the answers

What is the purpose of ROS rescue assays?

To measure the survival of cancer cells under oxidative stress Signup and view all the answers

What is the role of AXL and CAV-1 in cancer cells?

They play a role in the sensitivity of MTH1 inhibitor TH1579 Signup and view all the answers

What is the characteristic of dormant myeloma cells?

They have a unique niche-dependent transcriptome signature Signup and view all the answers

What is the primary application of CellROX reagents and kits?

ROS detection Signup and view all the answers

What is the name of the tyrosine kinase inhibitor used in the treatment of mice in the study?

Cabozantinib Signup and view all the answers

What was the main limitation of the coculture method used in the study?

It couldn't replicate the conditions exactly similar to those present at the niche Signup and view all the answers

What is the name of the enzyme that was inhibited in the study?

AXL Signup and view all the answers

What is the name of the bioluminescent image analysis system used in the study?

IVIS Signup and view all the answers

What is the future direction of the study?

To determine if ROS is a result of dormancy or dormancy is a result of increases ROS Signup and view all the answers