24 Questions
What is the projected number of new myeloma cases per year in the UK by 2038-2040?
8300
What is the main objective of the research in determining the role of MTH1 and AXL in Karonudib sensitivity?
To identify whether MTH1 and AXL contribute to Karonudib induction of ROS and apoptosis
What is the main function of MTH1 in cancer cells?
To remove oxidized nucleotide from dNTP pool and prevent oxidative DNA damage
What is the purpose of the Myeloma-Osteoblast co-culture methodology?
To induce dormancy in myeloma cells
What is the effect of inhibiting MTH1 in cancer cells?
It induces apoptosis in cancer cells
What is the target of Karonudib in cancer cells?
MTH1
What is the function of CellROX Green Flow assay in the research?
To measure ROS production
What is the purpose of including Palbociclib in the research?
To study the effects of CDK4/6 inhibition on myeloma cells
What is the role of AXL in cancer cells?
It is involved in cancer dormancy
What is the name of the disease being studied in this research?
Multiple Myeloma
What is the purpose of the Resazurin assay in the research?
To determine cell viability in response to Karonudib
What is the expected outcome of the research in MTH1-/- cells?
Resistance to Karonudib
What is the purpose of checking the effect of drug Karonudib in dormant AXL-/- and MTH1-/- cell lines?
To investigate the anti-tumor effects of Karonudib in cancer cells
What is the role of osteoclasts in myeloma cells?
They control the reactivation of dormant myeloma cells
What is the consequence of MTH1 inhibition in cancer cells?
It induces DNA strand breaks and genotoxic damage
What is the purpose of ROS rescue assays?
To measure the survival of cancer cells under oxidative stress
What is the role of AXL and CAV-1 in cancer cells?
They play a role in the sensitivity of MTH1 inhibitor TH1579
What is the characteristic of dormant myeloma cells?
They have a unique niche-dependent transcriptome signature
What is the primary application of CellROX reagents and kits?
ROS detection
What is the name of the tyrosine kinase inhibitor used in the treatment of mice in the study?
Cabozantinib
What was the main limitation of the coculture method used in the study?
It couldn't replicate the conditions exactly similar to those present at the niche
What is the name of the enzyme that was inhibited in the study?
AXL
What is the name of the bioluminescent image analysis system used in the study?
IVIS
What is the future direction of the study?
To determine if ROS is a result of dormancy or dormancy is a result of increases ROS
Assess your understanding of the effects of Karonudib on dormant cell lines, including AXL-/- and MTH1-/-, and its role in cancer treatment. Compare the results of assays in different cell lines and understand the significance of ROS rescue assays. Learn about the role of AXL and CAV-1 in MTH1 inhibitor sensitivity.
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