Podcast
Questions and Answers
What are the general requirements for DNA replication?
What are the general requirements for DNA replication?
- Single-stranded DNA template, raw materials, enzymes (correct)
- Enzymes, proteins, and leading strands
- Double-stranded DNA template, raw materials, and proteins
- dNTPs, DNA polymerases, and Okazaki fragments
In which direction can DNA polymerases add new nucleotides?
In which direction can DNA polymerases add new nucleotides?
- 5' end
- Both 3' and 5' ends
- Neither 3' nor 5' ends
- 3' end (correct)
What is the term used for the short lengths of DNA produced by the discontinuous replication of the lagging strand?
What is the term used for the short lengths of DNA produced by the discontinuous replication of the lagging strand?
- Raw materials
- Okazaki fragments (correct)
- Leading strands
- Template strands
What is the process where DNA must be synthesized in short stretches on the lagging strand called?
What is the process where DNA must be synthesized in short stretches on the lagging strand called?
Where does DNA replication start on the chromosome?
Where does DNA replication start on the chromosome?
Which enzyme is responsible for adding new nucleotides during DNA replication?
Which enzyme is responsible for adding new nucleotides during DNA replication?
What is the defining characteristic of the origin of replication in bacteria?
What is the defining characteristic of the origin of replication in bacteria?
How does the DnaA protein contribute to replication initiation?
How does the DnaA protein contribute to replication initiation?
What is the role of DNA gyrase in DNA replication?
What is the role of DNA gyrase in DNA replication?
Which enzyme unwinds approximately 100 base pairs per second during DNA replication?
Which enzyme unwinds approximately 100 base pairs per second during DNA replication?
What happens when too much torsional stress is generated during DNA replication?
What happens when too much torsional stress is generated during DNA replication?
What is the main function of topoisomerases in DNA replication?
What is the main function of topoisomerases in DNA replication?
What is the size of DNA fragments resulting from the BamHI+PstI cuts?
What is the size of DNA fragments resulting from the BamHI+PstI cuts?
During DNA replication, which enzyme is responsible for synthesizing the new DNA strand?
During DNA replication, which enzyme is responsible for synthesizing the new DNA strand?
In DNA replication, which process involves the creation of Okazaki fragments?
In DNA replication, which process involves the creation of Okazaki fragments?
Which model of DNA replication was supported by the Meselson and Stahl experiment?
Which model of DNA replication was supported by the Meselson and Stahl experiment?
What is the error rate in E. coli DNA replication?
What is the error rate in E. coli DNA replication?
Which enzyme is responsible for joining Okazaki fragments in DNA replication?
Which enzyme is responsible for joining Okazaki fragments in DNA replication?
