18 Questions
What are the general requirements for DNA replication?
Single-stranded DNA template, raw materials, enzymes
In which direction can DNA polymerases add new nucleotides?
3' end
What is the term used for the short lengths of DNA produced by the discontinuous replication of the lagging strand?
Okazaki fragments
What is the process where DNA must be synthesized in short stretches on the lagging strand called?
Discontinuous replication
Where does DNA replication start on the chromosome?
'Origin of Replication'
Which enzyme is responsible for adding new nucleotides during DNA replication?
DNA polymerase
What is the defining characteristic of the origin of replication in bacteria?
Presence of an A+T-rich region
How does the DnaA protein contribute to replication initiation?
Inducing DNA bending and twisting
What is the role of DNA gyrase in DNA replication?
Creates a double-strand break in DNA
Which enzyme unwinds approximately 100 base pairs per second during DNA replication?
DNA helicase
What happens when too much torsional stress is generated during DNA replication?
Additional twisting of the helix occurs
What is the main function of topoisomerases in DNA replication?
Relieving torsional stress in DNA
What is the size of DNA fragments resulting from the BamHI+PstI cuts?
1000, 1500 bp
During DNA replication, which enzyme is responsible for synthesizing the new DNA strand?
DNA polymerase
In DNA replication, which process involves the creation of Okazaki fragments?
Lagging strand synthesis
Which model of DNA replication was supported by the Meselson and Stahl experiment?
Semi-conservative replication
What is the error rate in E. coli DNA replication?
~1 error per million nucleotides
Which enzyme is responsible for joining Okazaki fragments in DNA replication?
DNA ligase
Test your knowledge on the requirements of DNA replication such as templates, raw materials, and enzymes involved in the process. Understand concepts like dNTPs and the role of enzymes in assembling new nucleotide strands.
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