DNA Replication Requirements Quiz
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Questions and Answers

What are the general requirements for DNA replication?

  • Single-stranded DNA template, raw materials, enzymes (correct)
  • Enzymes, proteins, and leading strands
  • Double-stranded DNA template, raw materials, and proteins
  • dNTPs, DNA polymerases, and Okazaki fragments
  • In which direction can DNA polymerases add new nucleotides?

  • 5' end
  • Both 3' and 5' ends
  • Neither 3' nor 5' ends
  • 3' end (correct)
  • What is the term used for the short lengths of DNA produced by the discontinuous replication of the lagging strand?

  • Raw materials
  • Okazaki fragments (correct)
  • Leading strands
  • Template strands
  • What is the process where DNA must be synthesized in short stretches on the lagging strand called?

    <p>Discontinuous replication</p> Signup and view all the answers

    Where does DNA replication start on the chromosome?

    <p>'Origin of Replication'</p> Signup and view all the answers

    Which enzyme is responsible for adding new nucleotides during DNA replication?

    <p>DNA polymerase</p> Signup and view all the answers

    What is the defining characteristic of the origin of replication in bacteria?

    <p>Presence of an A+T-rich region</p> Signup and view all the answers

    How does the DnaA protein contribute to replication initiation?

    <p>Inducing DNA bending and twisting</p> Signup and view all the answers

    What is the role of DNA gyrase in DNA replication?

    <p>Creates a double-strand break in DNA</p> Signup and view all the answers

    Which enzyme unwinds approximately 100 base pairs per second during DNA replication?

    <p>DNA helicase</p> Signup and view all the answers

    What happens when too much torsional stress is generated during DNA replication?

    <p>Additional twisting of the helix occurs</p> Signup and view all the answers

    What is the main function of topoisomerases in DNA replication?

    <p>Relieving torsional stress in DNA</p> Signup and view all the answers

    What is the size of DNA fragments resulting from the BamHI+PstI cuts?

    <p>1000, 1500 bp</p> Signup and view all the answers

    During DNA replication, which enzyme is responsible for synthesizing the new DNA strand?

    <p>DNA polymerase</p> Signup and view all the answers

    In DNA replication, which process involves the creation of Okazaki fragments?

    <p>Lagging strand synthesis</p> Signup and view all the answers

    Which model of DNA replication was supported by the Meselson and Stahl experiment?

    <p>Semi-conservative replication</p> Signup and view all the answers

    What is the error rate in E. coli DNA replication?

    <p>~1 error per million nucleotides</p> Signup and view all the answers

    Which enzyme is responsible for joining Okazaki fragments in DNA replication?

    <p>DNA ligase</p> Signup and view all the answers

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