DNA Extraction Methods from Plants

<p>To remove contaminants (C)</p> Signup and view all the answers

<p>To remove red blood cells (C)</p> Signup and view all the answers

<p>To release DNA into the solution. (A)</p> Signup and view all the answers

<p>SDS or Triton X-100 (B)</p> Signup and view all the answers

<p>To digest RNAs that could interfere with DNA extraction. (B)</p> Signup and view all the answers

<p>A white, stringy material. (B)</p> Signup and view all the answers

<p>To remove residual salts and contaminants. (B)</p> Signup and view all the answers

<p>Use homogenization in conjunction with lysis buffer. (B)</p> Signup and view all the answers

<p>It inhibits DNases which could degrade DNA. (D)</p> Signup and view all the answers

<p>To study and analyze the isolated DNA. (C)</p> Signup and view all the answers

<p>To provide a hypotonic solution that disrupts RBCs (B)</p> Signup and view all the answers

<p>Potassium bicarbonate (B)</p> Signup and view all the answers

<p>Inhibiting DNases (A)</p> Signup and view all the answers

<p>To disrupt the cell membrane (D)</p> Signup and view all the answers

<p>Adding ammonium acetate (D)</p> Signup and view all the answers

<p>To precipitate DNA from the supernatant (C)</p> Signup and view all the answers

<p>They swell and eventually burst (D)</p> Signup and view all the answers

<p>Centrifugation (B)</p> Signup and view all the answers

<p>To separate phases (D)</p> Signup and view all the answers

<p>Organic Phase, Interface, Aqueous Phase (B)</p> Signup and view all the answers

<p>Add ice-cold ethanol (C)</p> Signup and view all the answers

<p>To remove residual salts and contaminants (C)</p> Signup and view all the answers

<p>Purify the DNA (B)</p> Signup and view all the answers

<p>TE buffer (Tris-EDTA) (C)</p> Signup and view all the answers

<p>Add lysis buffer and grind the tissue (C)</p> Signup and view all the answers

<p>It precipitates out of the solution (B)</p> Signup and view all the answers

<p>Wash DNA (A)</p> Signup and view all the answers

<p>It improves phase separation by reducing foaming (A)</p> Signup and view all the answers

<p>It separates the DNA from the cells (D)</p> Signup and view all the answers

<p>To denature proteins and dissolve lipids (D)</p> Signup and view all the answers

<p>TE buffer or water (C)</p> Signup and view all the answers

<p>Cellular lipid extraction (C)</p> Signup and view all the answers

<p>To maintain DNA's structural integrity (A)</p> Signup and view all the answers

A process of isolating DNA from a blood sample containing red blood cells, white blood cells, and platelets.

The process of removing red blood cells (RBCs) from a blood sample using a specific buffer.

The solid mass of DNA collected during the purification process after removing contaminants and alcohol.

Using a 70% ethanol solution to remove residual contaminants from the DNA pellet during the extraction process, leaving a purified DNA pellet.

A buffer solution used to dissolve and store purified DNA for future use.

The process of isolating DNA from cells for study.

Breaking open cells to release DNA.

A solution used to break open cells.

A substance that disrupts cell membranes during lysis.

Getting rid of proteins and other cellular material to isolate DNA.

Making DNA come out of solution, forming a pellet.

Removing remaining salts and contaminants from the DNA.

Extracting DNA from plant cells using specific steps to isolate the DNA

A classic and widely used DNA isolation technique using phenol, chloroform, and isoamyl alcohol to separate and purify DNA.

In the PCI method, phenol denatures and precipitates proteins, making them hydrophobic and readily separated from DNA.

Chloroform enhances phase separation between water and organic solvents, improving the clarity and efficiency of DNA isolation.

Isoamyl alcohol reduces foaming during the mixing of phenol and chloroform, improving the extraction process.

The process of isolating DNA from cultured animal cells or animal tissues, often using the PCI method.

The PCI method is preferred for its effectiveness in producing a large amount of pure and intact DNA, which is essential for various applications.

The PCI method is widely used because of its ability to produce high-quality DNA with excellent yield.

Phenol interacts with proteins in the cell lysate, causing them to become hydrophobic and precipitate out of solution, while DNA remains in solution.

A solution containing ammonium chloride, bicarbonate, and EDTA, designed to selectively break open red blood cells (RBCs) without harming white blood cells (WBCs).

A solution with a lower concentration of solutes compared to the cell's internal environment, causing water to move into the cell, leading to swelling and potential lysis (bursting).

EDTA, an anticoagulant, prevents the degradation of DNA by inhibiting DNases in the Lysis Buffer.

A process of separating components of a mixture based on their density by spinning them at high speed.

A solution containing detergent (like SDS) to disrupt cell membranes and release DNA, EDTA to inhibit DNases, and salt (NaCl) to facilitate DNA precipitation.

Ammonium acetate is added to the lysate after the WBC lysis to precipitate proteins, making them clump together and settle at the bottom during centrifugation.

A buffer solution containing Tris (a base) and EDTA (a chelator) used to dissolve and store purified DNA, preventing degradation and maintaining its stability.

A mixture of these organic solvents used to separate DNA from proteins and other cellular debris during DNA extraction. The phases separate into distinct layers, with DNA residing in the aqueous phase.

The water-based layer in the DNA extraction process where the DNA is collected after the PCIA treatment.

The boundary between the different layers formed during the PCIA extraction process. It's a thin layer that separates the aqueous phase from the organic phases.

The layer containing the organic solvents (phenol, chloroform, isoamyl alcohol) that are used to remove proteins and other contaminants during DNA extraction.

The process of adding cold ethanol to the aqueous phase, causing the DNA to become insoluble and clump together as a pellet.