18 Questions
What is one of the properties of CTAB mentioned in the text?
Corrosive and toxic
What role does liquid nitrogen play in plant DNA extraction?
Activates enzymes that cleave DNA into small pieces
How does CTAB help in DNA extraction from plant cells?
By binding with cell wall components and co-precipitating macromolecules
Which combination of methods is used to prepare plant samples before DNA extraction?
Grinding and Liquid nitrogen treatment
Why is liquid nitrogen added during plant tissue grinding?
To prevent enzymatic reactions and freeze the tissue instantly
Why is CTAB considered useful in DNA extraction from plant cells?
To bind with macromolecules for removal
What chemicals are commonly used in organic solvent-based DNA extraction?
Phenol and chloroform
Which method of DNA extraction uses salts like sodium chloride, potassium acetate, and ammonium acetate?
Salting out DNA extraction method
Why is the phenol-chloroform method restricted in the organic solvent-based DNA extraction?
Harmful nature of phenol and chloroform
What is the drawback of the proteinase K DNA extraction method?
Time-consuming
Which chemical is not commonly used in the chemical-based DNA extraction methods mentioned?
Proteinase K
What is the purpose of using organic solvents in DNA extraction?
To break down the cell and precipitate other macromolecules
What is the role of polyvinyl pyrrolidone (PVP) in plant DNA extraction?
It binds to the phenolic ring of tannin, preventing its interaction with DNA.
Which compound plays a supporting role in preventing the oxidation of polyphenolic compounds during plant DNA extraction?
Beta-mercaptoethanol
What is the main ingredient, along with CTAB, for plants containing a high level of tannin or polyphenolic compounds?
Polyvinyl pyrrolidone (PVP)
Which ingredient varies in the preparation of CTAB buffer for plant DNA extraction depending on different plant species?
CTAB
What is the role of beta-mercaptoethanol in the method of plant DNA extraction?
It acts as an antioxidant, preventing oxidation of compounds.
Which compound is mainly responsible for binding to tannin and preventing its interaction with DNA in plant DNA extraction?
Polyvinyl pyrrolidone (PVP)
Learn about the process of DNA extraction from plant tissues rich in tannins, where polyphenolic compounds can inhibit PCR reactions. Discover how Polyvinyl Pyrrolidone (PVP) and Beta-mercaptoethanol play crucial roles in overcoming this challenge.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
