DNA Extraction from Plant Tissues with Tannins

Play an AI-generated podcast conversation about this lesson

What is one of the properties of CTAB mentioned in the text?

Soluble in water but not in alcohol
Non-corrosive and non-toxic
Insoluble in water and alcohol
Corrosive and toxic (correct)

What role does liquid nitrogen play in plant DNA extraction?

Is used to heat the tissue during grinding
Activates enzymes that cleave DNA into small pieces (correct)
Slows down the grinding process
Induces enzymatic reactions

How does CTAB help in DNA extraction from plant cells?

By inhibiting the enzymes responsible for DNA cleavage
By dissolving only polar compounds
By binding with cell wall components and co-precipitating macromolecules (correct)
By only dissolving non-polar compounds

Which combination of methods is used to prepare plant samples before DNA extraction?

Grinding and Liquid nitrogen treatment (C) Signup and view all the answers

Why is liquid nitrogen added during plant tissue grinding?

To prevent enzymatic reactions and freeze the tissue instantly (A) Signup and view all the answers

Why is CTAB considered useful in DNA extraction from plant cells?

To bind with macromolecules for removal (C) Signup and view all the answers

What chemicals are commonly used in organic solvent-based DNA extraction?

Phenol and chloroform (B) Signup and view all the answers

Which method of DNA extraction uses salts like sodium chloride, potassium acetate, and ammonium acetate?

Salting out DNA extraction method (B) Signup and view all the answers

Why is the phenol-chloroform method restricted in the organic solvent-based DNA extraction?

Harmful nature of phenol and chloroform (A) Signup and view all the answers

What is the drawback of the proteinase K DNA extraction method?

Time-consuming (C) Signup and view all the answers

Which chemical is not commonly used in the chemical-based DNA extraction methods mentioned?

Proteinase K (B) Signup and view all the answers

What is the purpose of using organic solvents in DNA extraction?

To break down the cell and precipitate other macromolecules (A) Signup and view all the answers

What is the role of polyvinyl pyrrolidone (PVP) in plant DNA extraction?

It binds to the phenolic ring of tannin, preventing its interaction with DNA. (A) Signup and view all the answers

Which compound plays a supporting role in preventing the oxidation of polyphenolic compounds during plant DNA extraction?

Beta-mercaptoethanol (D) Signup and view all the answers

What is the main ingredient, along with CTAB, for plants containing a high level of tannin or polyphenolic compounds?

Polyvinyl pyrrolidone (PVP) (B) Signup and view all the answers

Which ingredient varies in the preparation of CTAB buffer for plant DNA extraction depending on different plant species?

CTAB (D) Signup and view all the answers

What is the role of beta-mercaptoethanol in the method of plant DNA extraction?

It acts as an antioxidant, preventing oxidation of compounds. (B) Signup and view all the answers

Which compound is mainly responsible for binding to tannin and preventing its interaction with DNA in plant DNA extraction?