DNA Extraction from Plant Tissues with Tannins
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Questions and Answers

What is one of the properties of CTAB mentioned in the text?

  • Soluble in water but not in alcohol
  • Non-corrosive and non-toxic
  • Insoluble in water and alcohol
  • Corrosive and toxic (correct)

    • What role does liquid nitrogen play in plant DNA extraction?

  • Is used to heat the tissue during grinding
  • Activates enzymes that cleave DNA into small pieces (correct)
  • Slows down the grinding process
  • Induces enzymatic reactions

    • How does CTAB help in DNA extraction from plant cells?

  • By inhibiting the enzymes responsible for DNA cleavage
  • By dissolving only polar compounds
  • By binding with cell wall components and co-precipitating macromolecules (correct)
  • By only dissolving non-polar compounds

    • Which combination of methods is used to prepare plant samples before DNA extraction?

    <p>Grinding and Liquid nitrogen treatment</p> Signup and view all the answers

    Why is liquid nitrogen added during plant tissue grinding?

    <p>To prevent enzymatic reactions and freeze the tissue instantly</p> Signup and view all the answers

    Why is CTAB considered useful in DNA extraction from plant cells?

    <p>To bind with macromolecules for removal</p> Signup and view all the answers

    What chemicals are commonly used in organic solvent-based DNA extraction?

    <p>Phenol and chloroform</p> Signup and view all the answers

    Which method of DNA extraction uses salts like sodium chloride, potassium acetate, and ammonium acetate?

    <p>Salting out DNA extraction method</p> Signup and view all the answers

    Why is the phenol-chloroform method restricted in the organic solvent-based DNA extraction?

    <p>Harmful nature of phenol and chloroform</p> Signup and view all the answers

    What is the drawback of the proteinase K DNA extraction method?

    <p>Time-consuming</p> Signup and view all the answers

    Which chemical is not commonly used in the chemical-based DNA extraction methods mentioned?

    <p>Proteinase K</p> Signup and view all the answers

    What is the purpose of using organic solvents in DNA extraction?

    <p>To break down the cell and precipitate other macromolecules</p> Signup and view all the answers

    What is the role of polyvinyl pyrrolidone (PVP) in plant DNA extraction?

    <p>It binds to the phenolic ring of tannin, preventing its interaction with DNA.</p> Signup and view all the answers

    Which compound plays a supporting role in preventing the oxidation of polyphenolic compounds during plant DNA extraction?

    <p>Beta-mercaptoethanol</p> Signup and view all the answers

    What is the main ingredient, along with CTAB, for plants containing a high level of tannin or polyphenolic compounds?

    <p>Polyvinyl pyrrolidone (PVP)</p> Signup and view all the answers

    Which ingredient varies in the preparation of CTAB buffer for plant DNA extraction depending on different plant species?

    <p>CTAB</p> Signup and view all the answers

    What is the role of beta-mercaptoethanol in the method of plant DNA extraction?

    <p>It acts as an antioxidant, preventing oxidation of compounds.</p> Signup and view all the answers

    Which compound is mainly responsible for binding to tannin and preventing its interaction with DNA in plant DNA extraction?

    <p>Polyvinyl pyrrolidone (PVP)</p> Signup and view all the answers

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