Plant Genomic DNA Extraction
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Questions and Answers

Why is isolating DNA from plant tissues challenging?

  • Plant tissues have variable levels of metabolites and structural biomolecules.
  • Polysaccharides and polyphenols in plant tissues interfere with DNA isolation.
  • Biochemistry between plant species is extremely different.
  • All of the above (correct)
  • Which class of plant biomolecules can significantly vary between species and interfere with DNA isolation?

  • Lipids and proteins
  • Nucleic acids and carbohydrates
  • Polysaccharides and polyphenols (correct)
  • Carotenoids and flavonoids
  • What are the hazards associated with using phenol and chloroform for DNA isolation?

  • Phenol can cause serious chemical burns, and chloroform is a known carcinogen. (correct)
  • Both phenol and chloroform are toxic if inhaled, and can cause skin irritation.
  • Phenol can lead to allergic reactions, and chloroform is highly flammable.
  • Phenol and chloroform can react violently if mixed together.
  • When should the CTAB method using solid phase extraction be best used?

    <p>When smaller DNA fragment sizes are acceptable.</p> Signup and view all the answers

    Why are methods using phenol and chloroform preferred for the isolation of high molecular weight DNA?

    <p>They are considered more effective for high molecular weight DNA isolation.</p> Signup and view all the answers

    What is the main challenge in isolating DNA from plant tissues?

    <p>Contaminating polysaccharides and polyphenols</p> Signup and view all the answers

    Why are methods using phenol and chloroform preferred for isolating high molecular weight plant DNA?

    <p>They effectively remove contaminating polysaccharides and polyphenols</p> Signup and view all the answers

    Which hazard is associated with using phenol for DNA isolation?

    <p>It can cause very serious chemical burns</p> Signup and view all the answers

    Why is the CTAB method using solid phase extraction best used for assays where smaller DNA fragment sizes are acceptable?

    <p>It avoids hazardous chemicals</p> Signup and view all the answers

    Which class of plant biomolecules significantly varies between species and interferes with DNA isolation?

    <p>Polysaccharides and polyphenols</p> Signup and view all the answers

    Isolating DNA from plant tissues is more challenging than from animal tissues due to the varying levels of metabolites and structural biomolecules.

    <p>True</p> Signup and view all the answers

    The CTAB method using solid phase extraction is preferred for isolating high molecular weight plant DNA.

    <p>False</p> Signup and view all the answers

    Phenol can cause very serious chemical burns while chloroform is a known carcinogen.

    <p>True</p> Signup and view all the answers

    Polysaccharides and polyphenols are two classes of plant biomolecules that do not vary significantly between species.

    <p>False</p> Signup and view all the answers

    Methods using phenol and chloroform are considered hazardous, but are preferred for isolating high molecular weight DNA from plant tissues.

    <p>True</p> Signup and view all the answers

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