Podcast
Questions and Answers
Why is isolating DNA from plant tissues challenging?
Why is isolating DNA from plant tissues challenging?
- Plant tissues have variable levels of metabolites and structural biomolecules.
- Polysaccharides and polyphenols in plant tissues interfere with DNA isolation.
- Biochemistry between plant species is extremely different.
- All of the above (correct)
Which class of plant biomolecules can significantly vary between species and interfere with DNA isolation?
Which class of plant biomolecules can significantly vary between species and interfere with DNA isolation?
- Lipids and proteins
- Nucleic acids and carbohydrates
- Polysaccharides and polyphenols (correct)
- Carotenoids and flavonoids
What are the hazards associated with using phenol and chloroform for DNA isolation?
What are the hazards associated with using phenol and chloroform for DNA isolation?
- Phenol can cause serious chemical burns, and chloroform is a known carcinogen. (correct)
- Both phenol and chloroform are toxic if inhaled, and can cause skin irritation.
- Phenol can lead to allergic reactions, and chloroform is highly flammable.
- Phenol and chloroform can react violently if mixed together.
When should the CTAB method using solid phase extraction be best used?
When should the CTAB method using solid phase extraction be best used?
Why are methods using phenol and chloroform preferred for the isolation of high molecular weight DNA?
Why are methods using phenol and chloroform preferred for the isolation of high molecular weight DNA?
What is the main challenge in isolating DNA from plant tissues?
What is the main challenge in isolating DNA from plant tissues?
Why are methods using phenol and chloroform preferred for isolating high molecular weight plant DNA?
Why are methods using phenol and chloroform preferred for isolating high molecular weight plant DNA?
Which hazard is associated with using phenol for DNA isolation?
Which hazard is associated with using phenol for DNA isolation?
Why is the CTAB method using solid phase extraction best used for assays where smaller DNA fragment sizes are acceptable?
Why is the CTAB method using solid phase extraction best used for assays where smaller DNA fragment sizes are acceptable?
Which class of plant biomolecules significantly varies between species and interferes with DNA isolation?
Which class of plant biomolecules significantly varies between species and interferes with DNA isolation?
Isolating DNA from plant tissues is more challenging than from animal tissues due to the varying levels of metabolites and structural biomolecules.
Isolating DNA from plant tissues is more challenging than from animal tissues due to the varying levels of metabolites and structural biomolecules.
The CTAB method using solid phase extraction is preferred for isolating high molecular weight plant DNA.
The CTAB method using solid phase extraction is preferred for isolating high molecular weight plant DNA.
Phenol can cause very serious chemical burns while chloroform is a known carcinogen.
Phenol can cause very serious chemical burns while chloroform is a known carcinogen.
Polysaccharides and polyphenols are two classes of plant biomolecules that do not vary significantly between species.
Polysaccharides and polyphenols are two classes of plant biomolecules that do not vary significantly between species.
Methods using phenol and chloroform are considered hazardous, but are preferred for isolating high molecular weight DNA from plant tissues.
Methods using phenol and chloroform are considered hazardous, but are preferred for isolating high molecular weight DNA from plant tissues.
