20 Questions
What is the main purpose of DNA extraction?
To isolate DNA in a biological sample for further investigations
Where is genomic DNA located in eukaryotic cells?
Nucleus
What is the structure of DNA?
Double helical
What is the purpose of coiling the long linear DNA strands into a tightly packed structure?
To fit the DNA in a very small nucleus
Which macromolecules need to be separated from DNA during the extraction process?
Proteins, RNA, and lipids
In which field is DNA extraction particularly useful?
Forensics investigations
What does DNA extraction allow scientists to obtain?
DNA in a relatively purified form
Which biological samples can be used for DNA extraction in forensics investigations?
Blood, hair, and skin
Why is DNA considered the molecule of life?
All of the above
What are deoxynucleotides?
Polymers that make up each strand of DNA.
What is the main purpose of cell lysis in DNA extraction?
To release genomic DNA from the nucleus
What is the specific function of Sodium chloride in the cell lysis solution?
To osmotically shock the cells
What is the role of Sodium dodecyl Sulfate (SDS) in the cell lysis solution?
To destruct the cellular and nuclear membranes
What is the function of Ethylene diamine tetra acetic acid (EDTA) in the cell lysis solution?
To chelate metal ions that act as a cofactor for nucleases
What is the main purpose of Proteinase K in the cell lysis solution?
To digest histone and other proteins
What is the function of Tris-HCL in the cell lysis solution?
To maintain pH optimum for proteinase K activity
What is the purpose of adding Phenol: Chloroform: Isoamyl alcohol (25:24:1) in the purification stage of DNA extraction?
To promote partitioning of proteins and lipids into the organic phase
What is the main goal of deprotonation in DNA extraction?
To extract DNA from the protein-nucleic acid complex
Which ingredient in the cell lysis solution inhibits the function of nucleases to prevent DNA destruction?
(EDTA) Ethylene diamine tetra acetic acid
Which component promotes the partitioning of proteins and lipids into the organic phase during DNA extraction?
Phenol: Chloroform: Isoamyl alcohol (25:24:1)
Learn about the role of DNA as the molecule of life and its double helical structure, along with the location of genomic DNA in eukaryotic cells. Understand the importance of DNA in passing down instructions from parents to children.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
