Bioinformatics Lab PCR Optimization Quiz
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Questions and Answers

What is the purpose of optimizing PCR conditions?

  • To decrease the amplification specificity
  • To speed up the PCR process
  • To obtain the highest possible DNA yield (correct)
  • To reduce the cost of PCR reagents

    • Which primer pair is used for amplifying the partial 16S rRNA gene?

  • ITS1-ITS4
  • A571F-UA1406R
  • E334F-E519R
  • E8F-E939R (correct)

    • What is the purpose of using a GC-clamp in PCR?

  • To reduce the presence of GC-rich regions in the PCR product
  • To increase the annealing temperature of the primers
  • To prevent non-specific amplification of DNA (correct)
  • To improve the accuracy of DNA sequencing

    • Why are the PCR products run on agarose gel?

    <p>To visualize and confirm the presence of PCR products</p> Signup and view all the answers

    What is the purpose of PCR product purification?

    <p>To remove excess primers and nucleotides</p> Signup and view all the answers

    What is the purpose of using a GC-clamp in PCR?

    <p>To prevent non-specific amplification</p> Signup and view all the answers

    What is the purpose of PCR product purification?

    <p>To remove excess primers and nucleotides</p> Signup and view all the answers

    Why are the PCR products run on agarose gel?

    <p>To visualize and confirm the presence of PCR products</p> Signup and view all the answers

    What is the purpose of optimizing PCR conditions?

    <p>To ensure efficient and specific amplification of the target DNA sequence</p> Signup and view all the answers

    Which primer pair is used for amplifying the partial 16S rRNA gene?

    <p>E8F-E334F</p> Signup and view all the answers

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