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Questions and Answers
What is the purpose of optimizing PCR conditions?
What is the purpose of optimizing PCR conditions?
- To decrease the amplification specificity
- To speed up the PCR process
- To obtain the highest possible DNA yield (correct)
- To reduce the cost of PCR reagents
Which primer pair is used for amplifying the partial 16S rRNA gene?
Which primer pair is used for amplifying the partial 16S rRNA gene?
- ITS1-ITS4
- A571F-UA1406R
- E334F-E519R
- E8F-E939R (correct)
What is the purpose of using a GC-clamp in PCR?
What is the purpose of using a GC-clamp in PCR?
- To reduce the presence of GC-rich regions in the PCR product
- To increase the annealing temperature of the primers
- To prevent non-specific amplification of DNA (correct)
- To improve the accuracy of DNA sequencing
Why are the PCR products run on agarose gel?
Why are the PCR products run on agarose gel?
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What is the purpose of PCR product purification?
What is the purpose of PCR product purification?
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What is the purpose of using a GC-clamp in PCR?
What is the purpose of using a GC-clamp in PCR?
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What is the purpose of PCR product purification?
What is the purpose of PCR product purification?
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Why are the PCR products run on agarose gel?
Why are the PCR products run on agarose gel?
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What is the purpose of optimizing PCR conditions?
What is the purpose of optimizing PCR conditions?
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Which primer pair is used for amplifying the partial 16S rRNA gene?
Which primer pair is used for amplifying the partial 16S rRNA gene?
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