Podcast
Questions and Answers
What is the primary purpose of adding the detergent/salt solution during DNA extraction?
What is the primary purpose of adding the detergent/salt solution during DNA extraction?
- To provide a nutrient source
- To break down the cell membrane (correct)
- To precipitate the DNA
- To increase the temperature of the solution
Why is meat tenderizer added during the DNA extraction process?
Why is meat tenderizer added during the DNA extraction process?
- To help dissolve lipids in the membrane
- To lower the temperature of the solution
- To remove cellular proteins attached to DNA (correct)
- To precipitate DNA from the solution
What is the main function of ethanol in the DNA extraction process?
What is the main function of ethanol in the DNA extraction process?
- To make the cell membrane more permeable
- To maintain the temperature of the solution
- To precipitate the DNA and make it visible (correct)
- To neutralize the negative charge of DNA
In the bioengineering lab, what is the initial step in preparing agar plates?
In the bioengineering lab, what is the initial step in preparing agar plates?
What temperature is used to incubate the cells in the bioengineering lab?
What temperature is used to incubate the cells in the bioengineering lab?
What happens during the 'heat shock' phase of transforming cells?
What happens during the 'heat shock' phase of transforming cells?
What is the purpose of the selective marker added to S plates?
What is the purpose of the selective marker added to S plates?
What is the purpose of flipping the agar plates during incubation?
What is the purpose of flipping the agar plates during incubation?
What is the primary goal of introducing foreign DNA into bacterial cells?
What is the primary goal of introducing foreign DNA into bacterial cells?
What is the purpose of preparing a 2.0% agarose gel in electrophoresis?
What is the purpose of preparing a 2.0% agarose gel in electrophoresis?
What factor primarily influences how fast a dye migrates through the gel?
What factor primarily influences how fast a dye migrates through the gel?
Which of the following procedures is necessary to visualize the dye bands after electrophoresis?
Which of the following procedures is necessary to visualize the dye bands after electrophoresis?
Which outcome indicates a successful separation of dye components in the gel?
Which outcome indicates a successful separation of dye components in the gel?
What is the role of the electric current in the electrophoresis process?
What is the role of the electric current in the electrophoresis process?
How does Crystal Violet behave under certain conditions during electrophoresis?
How does Crystal Violet behave under certain conditions during electrophoresis?
Which aspect of the transformed cells is being observed after incubation?
Which aspect of the transformed cells is being observed after incubation?
What is a common application for the technique of electrophoresis in research and diagnostics?
What is a common application for the technique of electrophoresis in research and diagnostics?
What happens to larger or less negatively charged dyes during electrophoresis?
What happens to larger or less negatively charged dyes during electrophoresis?
Flashcards
DNA Extraction
DNA Extraction
Separating DNA from other cellular components of a strawberry.
Detergent's Role
Detergent's Role
Breaks down the cell membrane, releasing DNA.
Salt's Role in DNA extraction
Salt's Role in DNA extraction
Neutralizes DNA's negative charge, enabling strand formation.
Agar Plates
Agar Plates
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Heat Shock Transformation
Heat Shock Transformation
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Competent Cells
Competent Cells
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LB Agar
LB Agar
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Selective Marker
Selective Marker
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Bacterial Transformation
Bacterial Transformation
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Selective Plates
Selective Plates
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Agarose Gel Electrophoresis
Agarose Gel Electrophoresis
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Agarose Gel
Agarose Gel
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Electrophoresis
Electrophoresis
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Rainbow Pattern
Rainbow Pattern
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Dyes in Electrophoresis
Dyes in Electrophoresis
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Incubation Temperature
Incubation Temperature
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LB Media
LB Media
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Genetic Transformation
Genetic Transformation
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Study Notes
DNA Extraction Lab
- Strawberry DNA Extraction: Strawberries are ground to release cell contents.
- Detergent/Salt Solution: Breaks down cell membranes (lipids), neutralizes DNA's negative charge, allowing strand formation.
- Meat Tenderizer (Enzyme): Breaks down proteins attached to DNA.
- Ethanol: Precipitates DNA, making it visible. Cold ethanol prevents DNA from dissolving.
Bioengineering Lab: Bacterial Transformation
- Agar Plates: Prepared with LB agar, adding a selective marker (pill) to certain plates.
- Growing Blank Cells: Bacterial cells are streaked onto agar plates (NS, S-) to culture.
- Making Cells Competent: Cells are prepared using cold temperature for easier DNA uptake.
- Transforming Cells: Foreign DNA is added to competent cells; a heat shock step facilitates DNA entry.
- Recovering Cells: Cells are placed in LB media to recover and express the introduced DNA.
- Plating Cells: Transformed cells are spread on selective plates.
- Incubating Cells: Cells are grown at 37°C, allowing comparison between transformed and non-transformed cells.
Electrophoresis Lab: Dye Separation
- Gel Preparation: Agarose gel is made for separating dyes based on size and charge.
- Dye Loading: Dye samples are loaded into designated wells. Precise volumes (10 µL) are used.
- Electrophoresis: Electric current separates dyes in the gel based on size and charge. Smaller or negatively charged dyes migrate faster.
- Gel Visualization: Stained or unstained gel viewed under UV for separation analysis. The process creates a distinguishable pattern.
- Real-world Application: Commonly used for DNA, RNA, and protein analysis in research, quality control, and forensics. An electric current is employed to separate the dyes based on various properties. Crystal violet's movement to the negative side might indicate a positive charge.
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Description
This quiz covers the methods and processes involved in DNA extraction from strawberries and bacterial transformation using competent cells. You will learn about the chemicals and steps necessary to isolate and manipulate DNA. Test your knowledge on laboratory techniques crucial for bioengineering.
