7 Questions
What is the purpose of using restriction enzymes in genetic engineering?
To cut DNA at specific locations
Which enzyme is responsible for joining DNA fragments together in genetic engineering?
DNA ligase
How is the recombinant DNA molecule inserted into a host cell in genetic engineering?
Through a process called transformation
Why are selective markers, such as antibiotic resistance genes, used in the selection and screening of transformed cells?
To identify transformed cells that have taken up the recombinant DNA
What type of bonds does DNA ligase catalyze the formation of between DNA fragments?
Phosphodiester bonds
What happens to the sugar-phosphate backbones of adjacent DNA molecules during the action of DNA ligase?
They anneal together
What is the role of DNA ligase in the formation of a recombinant DNA molecule?
It repairs breaks in the sugar-phosphate backbone
This quiz covers the process of isolating genetic material (DNA) by breaking down the cell wall, cutting DNA at specific locations using restriction enzymes, and joining DNA fragments. Test your knowledge on DNA extraction and manipulation techniques.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
