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DNA Extraction and Manipulation Process

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Questions and Answers

What is the purpose of using restriction enzymes in genetic engineering?

To join DNA fragments together

To insert DNA into the host cell

To cut DNA at specific locations (correct)

To extract DNA from the cell

Which enzyme is responsible for joining DNA fragments together in genetic engineering?

Cellulose

Lysosomes

Chitinase

DNA ligase (correct)

How is the recombinant DNA molecule inserted into a host cell in genetic engineering?

Through the action of DNA ligase

By using restriction enzymes

By using cell wall digesting enzymes

Through a process called transformation (correct)

Why are selective markers, such as antibiotic resistance genes, used in the selection and screening of transformed cells?

To identify transformed cells that have taken up the recombinant DNA Signup and view all the answers

What type of bonds does DNA ligase catalyze the formation of between DNA fragments?

Phosphodiester bonds Signup and view all the answers

What happens to the sugar-phosphate backbones of adjacent DNA molecules during the action of DNA ligase?

They anneal together Signup and view all the answers

What is the role of DNA ligase in the formation of a recombinant DNA molecule?

It repairs breaks in the sugar-phosphate backbone Signup and view all the answers

DNA Extraction and Manipulation Process

Choose a study mode

Podcast

Questions and Answers

What is the purpose of using restriction enzymes in genetic engineering?

Which enzyme is responsible for joining DNA fragments together in genetic engineering?

How is the recombinant DNA molecule inserted into a host cell in genetic engineering?

Why are selective markers, such as antibiotic resistance genes, used in the selection and screening of transformed cells?

What type of bonds does DNA ligase catalyze the formation of between DNA fragments?

What happens to the sugar-phosphate backbones of adjacent DNA molecules during the action of DNA ligase?

What is the role of DNA ligase in the formation of a recombinant DNA molecule?

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