Degenerative Cervical Myelopathy (DCM) and APOE Carrier Status
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Degenerative Cervical Myelopathy (DCM) and APOE Carrier Status

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Questions and Answers

The Cre-lox system involves the use of two key components: the _______________________ enzyme and specific DNA sequences called loxP sites.

Cre recombinase

Viral delivery of the ______ constructs can be used for expression and siRNA/shRNA mediated knockdowns.

loxP/Frt

Region specific ablation of pericytes can be achieved using ______-DTR-loxP.

AAV2-CAG

The activity of the Cre recombinase enzyme is controlled by the substrate _______________________.

<p>tamoxifen</p> Signup and view all the answers

The Cre-lox system is used for _______________________ deletion of genes at a specific time.

<p>conditional</p> Signup and view all the answers

The ______-Cre mouse can be used to express a gene in excitatory neurons that innervate the forelimb.

<p>VGlut2</p> Signup and view all the answers

The ______-lox system is used for conditional gene expression.

<p>Cre</p> Signup and view all the answers

The Rosa26 locus is a commonly used genetic site for targeted _______________________ and genetic manipulation.

<p>gene expression</p> Signup and view all the answers

Viral intersectional techniques can be achieved using ______-Flp and Vgat-Cre double transgenic mice.

<p>VGlut2</p> Signup and view all the answers

The D1R-Cre;D2R-Flp double transgenic offspring were generated by crossing the lines with ______ mice

<p>tdTomato-LoxP; eGFP-Frt</p> Signup and view all the answers

The DTR-mediated cell ablation system allows for the selective ablation of cell types through the administration of _______________________.

<p>diphtheria toxin</p> Signup and view all the answers

The ______-Frt system is used for conditional gene expression in viral vectors.

<p>Flp</p> Signup and view all the answers

The D1R cells would be ______ after tam administration in the double reporter mice

<p>red</p> Signup and view all the answers

The Flp-Frt system is similar to the Cre-lox system, but lacks a chemical trigger, such as _______________________.

<p>tamoxifen</p> Signup and view all the answers

The conditional expression lines follow the nomenclature of Promoter-______-R26-CAG-Transgene-loxP

<p>Cre</p> Signup and view all the answers

The FLP recombinase recognizes specific DNA sequences called _______________________ sites.

<p>FRT</p> Signup and view all the answers

The Crispr-Cas9 system is used for _______________________ gene editing.

<p>precise</p> Signup and view all the answers

The Cre/loxP system is used for ______ gene expression

<p>conditional</p> Signup and view all the answers

The DTR-mediated cell ablation lines follow the nomenclature of Promoter-______-R26-CAG-DTR-loxP

<p>Cre</p> Signup and view all the answers

The Cre-lox system can be used for conditional gene _______________________ at a specific time.

<p>expression</p> Signup and view all the answers

The combination of Cre-lox and Flp-Frt systems allows for the intersectional manipulation of multiple cell populations through _______________________ genetics.

<p>dual recombinase</p> Signup and view all the answers

The Flp/Frt system is used for ______ reporter lines

<p>conditional</p> Signup and view all the answers

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