Podcast
Questions and Answers
The Cre-lox system involves the use of two key components: the _______________________ enzyme and specific DNA sequences called loxP sites.
Cre recombinase
Viral delivery of the ______ constructs can be used for expression and siRNA/shRNA mediated knockdowns.
loxP/Frt
Region specific ablation of pericytes can be achieved using ______-DTR-loxP.
AAV2-CAG
The activity of the Cre recombinase enzyme is controlled by the substrate _______________________.
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The Cre-lox system is used for _______________________ deletion of genes at a specific time.
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The ______-Cre mouse can be used to express a gene in excitatory neurons that innervate the forelimb.
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The ______-lox system is used for conditional gene expression.
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The Rosa26 locus is a commonly used genetic site for targeted _______________________ and genetic manipulation.
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Viral intersectional techniques can be achieved using ______-Flp and Vgat-Cre double transgenic mice.
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The D1R-Cre;D2R-Flp double transgenic offspring were generated by crossing the lines with ______ mice
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The DTR-mediated cell ablation system allows for the selective ablation of cell types through the administration of _______________________.
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The ______-Frt system is used for conditional gene expression in viral vectors.
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The D1R cells would be ______ after tam administration in the double reporter mice
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The Flp-Frt system is similar to the Cre-lox system, but lacks a chemical trigger, such as _______________________.
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The conditional expression lines follow the nomenclature of Promoter-______-R26-CAG-Transgene-loxP
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The FLP recombinase recognizes specific DNA sequences called _______________________ sites.
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The Crispr-Cas9 system is used for _______________________ gene editing.
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The Cre/loxP system is used for ______ gene expression
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The DTR-mediated cell ablation lines follow the nomenclature of Promoter-______-R26-CAG-DTR-loxP
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The Cre-lox system can be used for conditional gene _______________________ at a specific time.
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The combination of Cre-lox and Flp-Frt systems allows for the intersectional manipulation of multiple cell populations through _______________________ genetics.
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The Flp/Frt system is used for ______ reporter lines
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