22 Questions
What is the purpose of a 'reporter line' in mice?
To conditionally express fluorescent tags
What is the function of Cre in the Cre-loxP system?
To recombine DNA sequences
What is the result of conditional deletion of the cell cycling gene KRas in GLAST+ pericytes?
Prevention of pericyte proliferation
What is the advantage of using tamoxifen administration in conditional deletion?
It allows for timing control of gene deletion
What is the purpose of crossing D1R-Cre 2.D2R-Flp mice with double transgenic tdTomato-LoxP; eGFP-Frt mice?
To generate a 'dual' reporter line
What is the advantage of using viral approaches to label and deliver transgenes?
It allows for localized Cre/Flp dependent expression
What is the purpose of using the Cre-loxP system in viral gene delivery?
To allow for conditional expression or deletion of a gene
What is the role of the VGlut2-Cre mouse in the viral gene delivery approach?
To selectively express a gene in VGlut2-expressing neurons
What is the advantage of using a dual viral approach in conditional gene expression?
It provides more control over the specific cells targeted
What is the function of the NpHR gene in optogenetics?
To silence neuronal activity
What is the purpose of using the Flp-Frt system in viral gene delivery?
To allow for conditional expression or deletion of a gene
What is the goal of using viral intersectional techniques?
To concurrently activate and silence specific neurons
What is the primary function of the Cre recombinase enzyme in the Cre-lox system?
To recognize and catalyze recombination events between loxP sites
What is the role of the loxP sites in the Cre-lox system?
To flank the gene of interest
What is the purpose of the Rosa26 genetic locus in mice?
To facilitate targeted gene expression and genetic manipulation
What is the outcome of conditional expression of DTR in Pdgfrb+ pericytes?
The pericytes express the DTR receptor
What is the purpose of the Flp-Frt system?
To catalyze recombination events between FRT sites
What is the outcome of conditional deletion of Fn1 in Pdgfrb+ pericytes?
The pericytes stop expressing the Fn1 gene
What is the purpose of the Cre-lox system?
To catalyze recombination events between loxP sites
What is the advantage of using a dual recombinase strategy combining Cre-lox and Flp-Frt systems?
To selectively manipulate multiple cell types
What is the role of tamoxifen in the Cre-lox system?
To control the timing of conditional deletion or expression
What is the outcome of conditional ablation of DTR-expressing cells?
The cells undergo apoptosis
This quiz assesses your understanding of the DCM PICO framework, specifically the effect of APOE carrier status on disease progression in degenerative cervical myelopathy. It delves into the impact of carrier status on cells in the spinal cord and the primary cell type affected.
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