DCM PICO Framework: Impact of APOE Carrier Status on Disease Progression
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Questions and Answers

What is the purpose of a 'reporter line' in mice?

  • To deliver transgenes using viral approaches
  • To conditionally delete specific genes
  • To ablate specific cells
  • To conditionally express fluorescent tags (correct)
  • What is the function of Cre in the Cre-loxP system?

  • To express fluorescent tags
  • To recombine DNA sequences (correct)
  • To delete genes
  • To deliver transgenes
  • What is the result of conditional deletion of the cell cycling gene KRas in GLAST+ pericytes?

  • Increased proliferation of pericytes
  • Localized expression of Cre
  • Labeling of pericytes with fluorescent tags
  • Prevention of pericyte proliferation (correct)
  • What is the advantage of using tamoxifen administration in conditional deletion?

    <p>It allows for timing control of gene deletion</p> Signup and view all the answers

    What is the purpose of crossing D1R-Cre 2.D2R-Flp mice with double transgenic tdTomato-LoxP; eGFP-Frt mice?

    <p>To generate a 'dual' reporter line</p> Signup and view all the answers

    What is the advantage of using viral approaches to label and deliver transgenes?

    <p>It allows for localized Cre/Flp dependent expression</p> Signup and view all the answers

    What is the purpose of using the Cre-loxP system in viral gene delivery?

    <p>To allow for conditional expression or deletion of a gene</p> Signup and view all the answers

    What is the role of the VGlut2-Cre mouse in the viral gene delivery approach?

    <p>To selectively express a gene in VGlut2-expressing neurons</p> Signup and view all the answers

    What is the advantage of using a dual viral approach in conditional gene expression?

    <p>It provides more control over the specific cells targeted</p> Signup and view all the answers

    What is the function of the NpHR gene in optogenetics?

    <p>To silence neuronal activity</p> Signup and view all the answers

    What is the purpose of using the Flp-Frt system in viral gene delivery?

    <p>To allow for conditional expression or deletion of a gene</p> Signup and view all the answers

    What is the goal of using viral intersectional techniques?

    <p>To concurrently activate and silence specific neurons</p> Signup and view all the answers

    What is the primary function of the Cre recombinase enzyme in the Cre-lox system?

    <p>To recognize and catalyze recombination events between loxP sites</p> Signup and view all the answers

    What is the role of the loxP sites in the Cre-lox system?

    <p>To flank the gene of interest</p> Signup and view all the answers

    What is the purpose of the Rosa26 genetic locus in mice?

    <p>To facilitate targeted gene expression and genetic manipulation</p> Signup and view all the answers

    What is the outcome of conditional expression of DTR in Pdgfrb+ pericytes?

    <p>The pericytes express the DTR receptor</p> Signup and view all the answers

    What is the purpose of the Flp-Frt system?

    <p>To catalyze recombination events between FRT sites</p> Signup and view all the answers

    What is the outcome of conditional deletion of Fn1 in Pdgfrb+ pericytes?

    <p>The pericytes stop expressing the Fn1 gene</p> Signup and view all the answers

    What is the purpose of the Cre-lox system?

    <p>To catalyze recombination events between loxP sites</p> Signup and view all the answers

    What is the advantage of using a dual recombinase strategy combining Cre-lox and Flp-Frt systems?

    <p>To selectively manipulate multiple cell types</p> Signup and view all the answers

    What is the role of tamoxifen in the Cre-lox system?

    <p>To control the timing of conditional deletion or expression</p> Signup and view all the answers

    What is the outcome of conditional ablation of DTR-expressing cells?

    <p>The cells undergo apoptosis</p> Signup and view all the answers

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