DCM PICO Framework: Impact of APOE Carrier Status on Disease Progression

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What is the purpose of a 'reporter line' in mice?

To deliver transgenes using viral approaches
To conditionally delete specific genes
To ablate specific cells
To conditionally express fluorescent tags (correct)

What is the function of Cre in the Cre-loxP system?

To express fluorescent tags
To recombine DNA sequences (correct)
To delete genes
To deliver transgenes

What is the result of conditional deletion of the cell cycling gene KRas in GLAST+ pericytes?

Increased proliferation of pericytes
Localized expression of Cre
Labeling of pericytes with fluorescent tags
Prevention of pericyte proliferation (correct)

What is the advantage of using tamoxifen administration in conditional deletion?

It allows for timing control of gene deletion (D) Signup and view all the answers

What is the purpose of crossing D1R-Cre 2.D2R-Flp mice with double transgenic tdTomato-LoxP; eGFP-Frt mice?

To generate a 'dual' reporter line (B) Signup and view all the answers

What is the advantage of using viral approaches to label and deliver transgenes?

It allows for localized Cre/Flp dependent expression (A) Signup and view all the answers

What is the purpose of using the Cre-loxP system in viral gene delivery?

To allow for conditional expression or deletion of a gene (D) Signup and view all the answers

What is the role of the VGlut2-Cre mouse in the viral gene delivery approach?

To selectively express a gene in VGlut2-expressing neurons (B) Signup and view all the answers

What is the advantage of using a dual viral approach in conditional gene expression?

It provides more control over the specific cells targeted (B) Signup and view all the answers

What is the function of the NpHR gene in optogenetics?

To silence neuronal activity (A) Signup and view all the answers

What is the purpose of using the Flp-Frt system in viral gene delivery?

To allow for conditional expression or deletion of a gene (B) Signup and view all the answers

What is the goal of using viral intersectional techniques?

To concurrently activate and silence specific neurons (C) Signup and view all the answers

What is the primary function of the Cre recombinase enzyme in the Cre-lox system?

To recognize and catalyze recombination events between loxP sites (B) Signup and view all the answers

What is the role of the loxP sites in the Cre-lox system?

To flank the gene of interest (A) Signup and view all the answers

What is the purpose of the Rosa26 genetic locus in mice?

To facilitate targeted gene expression and genetic manipulation (B) Signup and view all the answers

What is the outcome of conditional expression of DTR in Pdgfrb+ pericytes?

The pericytes express the DTR receptor (B) Signup and view all the answers

What is the purpose of the Flp-Frt system?

To catalyze recombination events between FRT sites (D) Signup and view all the answers

What is the outcome of conditional deletion of Fn1 in Pdgfrb+ pericytes?

The pericytes stop expressing the Fn1 gene (D) Signup and view all the answers

What is the purpose of the Cre-lox system?

To catalyze recombination events between loxP sites (D) Signup and view all the answers

What is the advantage of using a dual recombinase strategy combining Cre-lox and Flp-Frt systems?

To selectively manipulate multiple cell types (B) Signup and view all the answers

What is the role of tamoxifen in the Cre-lox system?

To control the timing of conditional deletion or expression (B) Signup and view all the answers

What is the outcome of conditional ablation of DTR-expressing cells?

The cells undergo apoptosis (C) Signup and view all the answers