Cs-Cys-PN Polymer Synthesis and Characterization

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Questions and Answers

What was the absorption peak at 2907 cm−1 primarily attributed to in the Cs-Cys-PN hydrogel?

Stretching vibrations for N–H of Cs-Cys-PN

Stretching vibrations for C–H of NIPAM (correct)

Stretching vibrations for C–O of NIPAM

Stretching vibrations for hydroxyl of Cs

Which characteristic absorption peak is associated with the amino stretching vibration of chitosan?

2876 cm−1

1650 cm−1

3450 cm−1

1620 cm−1 (correct)

What is the grafting rate of NIPAM to Cs-Cys reported to be?

60.7%

50.3%

45.6% (correct)

76.8%

What was indicated by the absorption peak at 2559 cm−1 in the synthesized Cs-Cys-PN?

Successful coupling of Cys Signup and view all the answers

Which method was used to analyze the lyophilized powder of Cs-Cys-PN?

Fourier Transform Infrared Spectroscopy Signup and view all the answers

What was the yield percentage for the synthesis of Cs-Cys-PN?

76.8% Signup and view all the answers

Which stretching vibrations were observed from the absorption peak at 3450 cm−1?

Hydroxyl of Cs Signup and view all the answers

What was the purpose of gold sputtering in the analysis of the Cs-Cys-PN hydrogel?

To improve SEM imaging Signup and view all the answers

What is the role of N-hydroxysuccinimide (NHS) in the synthesis process?

To activate carboxylic groups for amine coupling Signup and view all the answers

What is measured to determine the inhibition rate in the experiment?

The difference between the control group diameter and the treated sample diameter Signup and view all the answers

Which chemical element showed the greatest mean value difference between Cs-Cys and Cs-Cys-PN?

Nitrogen (N) Signup and view all the answers

What is the purpose of dialysis in the synthesis of Chitosan?

To remove unreacted reagents and small molecules Signup and view all the answers

What technique was used to observe the morphology of S. aureus?

Scanning electron microscopy (SEM) Signup and view all the answers

Why is the reaction performed under a nitrogen atmosphere?

To prevent oxidation of -SH groups Signup and view all the answers

What was the duration of co-culturing S. aureus with Cs-Cys-PN/PA in the experiment?

12 hours Signup and view all the answers

What is the significance of using 15 μL of MTT solution in cell viability tests?

To measure the metabolic activity of the cells Signup and view all the answers

Which component is important for modifications in the synthesis of Cs-Cys-PN?

N-isopropylacrylamide (NIPAM) Signup and view all the answers

Which reagent was used to dissolve the formazan crystals for absorbance measurement?

DMSO Signup and view all the answers

What was the purpose of fixing the samples with glutaraldehyde?

To preserve the morphology of the samples Signup and view all the answers

What does the assay at 492 nm measure in the context of cell viability?

Amount of formazan produced by viable cells Signup and view all the answers

What is the role of ethanol in the sample preparation process?

To dehydrate the samples Signup and view all the answers

What is the main component of the hydrogel synthesized in the described process?

Chitosan Signup and view all the answers

What role does the confocal laser scanning microscope (CLSM) play in the study of cellular uptake?

It provides imaging of the cells to visualize treatment effects Signup and view all the answers

Which morphological aspect was primarily studied in the paper?

Morphological characteristics of S. aureus Signup and view all the answers

Study Notes

Cs-Cys-PN/PA Synthesis

The synthesis of the Cs-Cys-PN polymer is described in the paper.
Cs-Cys-PN is a hydrogel formed by the reaction of chitosan (Cs), L-Cysteine (Cys), and N-isopropylacrylamide (NIPAM).
The synthesis process involves reacting Cs, NHS, and EDC to activate the Cs.
Cys is then added to the Cs solution, and the reaction takes place overnight under constant stirring.
The Cs-Cys product is purified through dialysis and lyophilized for future use.

Cs-Cys-PN/PA Characterization

The chemical structure and composition of Cs-Cys-PN were analyzed using Fourier Transform Infrared (FT-IR) spectroscopy and 1H Nuclear Magnetic Resonance (1H NMR) spectroscopy.
The results of the analysis indicated that the NIPAM had been successfully coupled to Cs-Cys.
FT-IR spectra of Cs-Cys-PN exhibited characteristic peaks corresponding to the stretching vibrations of the amide I and amide II bands of NIPAM, C–H of NIPAM, C–– O of NIPAM, and N–H of Cs-Cys-PN.
1H NMR spectra of Cs-Cys-PN showed characteristic peaks corresponding to the ring protons of Cs and the -CH of Cs-Cys, confirming the successful coupling of Cs-Cys.

Morphological Observation of Cs-Cys-PN/PA

The morphology of Cs-Cys-PN/PA was studied using scanning electron microscopy (SEM).
SEM images revealed the morphology of Cs-Cys-PN/PA as a porous network structure with spherical particles.
This structure provides a large surface area for potential interactions and applications.

Biological Activity of Cs-Cys-PN/PA

The study investigated the biocompatibility and antibacterial activity of Cs-Cys-PN/PA using different methods.
Cell viability was measured using the MTT assay, demonstrating that Cs-Cys-PN/PA exhibited good biocompatibility.
The antibacterial activity of Cs-Cys-PN/PA was evaluated against Staphylococcus aureus (S. aureus).
SEM images revealed morphological changes in S. aureus cells after treatment with Cs-Cys-PN/PA, indicating disruption of bacterial cell membranes and potential antimicrobial activity.
Confocal laser scanning microscopy (CLSM) was used to visualize the uptake of Cs-Cys-PN/PA by Hela cells.
The results showed that Cs-Cys-PN/PA was successfully internalized into the cells, suggesting potential applications in drug delivery.

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Description

This quiz covers the synthesis and characterization of the Cs-Cys-PN polymer, formed from chitosan, L-Cysteine, and N-isopropylacrylamide. You will explore the chemical reactions and analytical techniques used to assess the product's structure and composition. Perfect for students studying polymer chemistry and material science.