Cs-Cys-PN Polymer Synthesis and Characterization
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Questions and Answers

What was the absorption peak at 2907 cm−1 primarily attributed to in the Cs-Cys-PN hydrogel?

  • Stretching vibrations for N–H of Cs-Cys-PN
  • Stretching vibrations for C–H of NIPAM (correct)
  • Stretching vibrations for C–O of NIPAM
  • Stretching vibrations for hydroxyl of Cs
  • Which characteristic absorption peak is associated with the amino stretching vibration of chitosan?

  • 2876 cm−1
  • 1650 cm−1
  • 3450 cm−1
  • 1620 cm−1 (correct)
  • What is the grafting rate of NIPAM to Cs-Cys reported to be?

  • 60.7%
  • 50.3%
  • 45.6% (correct)
  • 76.8%
  • What was indicated by the absorption peak at 2559 cm−1 in the synthesized Cs-Cys-PN?

    <p>Successful coupling of Cys</p> Signup and view all the answers

    Which method was used to analyze the lyophilized powder of Cs-Cys-PN?

    <p>Fourier Transform Infrared Spectroscopy</p> Signup and view all the answers

    What was the yield percentage for the synthesis of Cs-Cys-PN?

    <p>76.8%</p> Signup and view all the answers

    Which stretching vibrations were observed from the absorption peak at 3450 cm−1?

    <p>Hydroxyl of Cs</p> Signup and view all the answers

    What was the purpose of gold sputtering in the analysis of the Cs-Cys-PN hydrogel?

    <p>To improve SEM imaging</p> Signup and view all the answers

    What is the role of N-hydroxysuccinimide (NHS) in the synthesis process?

    <p>To activate carboxylic groups for amine coupling</p> Signup and view all the answers

    What is measured to determine the inhibition rate in the experiment?

    <p>The difference between the control group diameter and the treated sample diameter</p> Signup and view all the answers

    Which chemical element showed the greatest mean value difference between Cs-Cys and Cs-Cys-PN?

    <p>Nitrogen (N)</p> Signup and view all the answers

    What is the purpose of dialysis in the synthesis of Chitosan?

    <p>To remove unreacted reagents and small molecules</p> Signup and view all the answers

    What technique was used to observe the morphology of S. aureus?

    <p>Scanning electron microscopy (SEM)</p> Signup and view all the answers

    Why is the reaction performed under a nitrogen atmosphere?

    <p>To prevent oxidation of -SH groups</p> Signup and view all the answers

    What was the duration of co-culturing S. aureus with Cs-Cys-PN/PA in the experiment?

    <p>12 hours</p> Signup and view all the answers

    What is the significance of using 15 μL of MTT solution in cell viability tests?

    <p>To measure the metabolic activity of the cells</p> Signup and view all the answers

    Which component is important for modifications in the synthesis of Cs-Cys-PN?

    <p>N-isopropylacrylamide (NIPAM)</p> Signup and view all the answers

    Which reagent was used to dissolve the formazan crystals for absorbance measurement?

    <p>DMSO</p> Signup and view all the answers

    What was the purpose of fixing the samples with glutaraldehyde?

    <p>To preserve the morphology of the samples</p> Signup and view all the answers

    What does the assay at 492 nm measure in the context of cell viability?

    <p>Amount of formazan produced by viable cells</p> Signup and view all the answers

    What is the role of ethanol in the sample preparation process?

    <p>To dehydrate the samples</p> Signup and view all the answers

    What is the main component of the hydrogel synthesized in the described process?

    <p>Chitosan</p> Signup and view all the answers

    What role does the confocal laser scanning microscope (CLSM) play in the study of cellular uptake?

    <p>It provides imaging of the cells to visualize treatment effects</p> Signup and view all the answers

    Which morphological aspect was primarily studied in the paper?

    <p>Morphological characteristics of S. aureus</p> Signup and view all the answers

    Study Notes

    Cs-Cys-PN/PA Synthesis

    • The synthesis of the Cs-Cys-PN polymer is described in the paper.
    • Cs-Cys-PN is a hydrogel formed by the reaction of chitosan (Cs), L-Cysteine (Cys), and N-isopropylacrylamide (NIPAM).
    • The synthesis process involves reacting Cs, NHS, and EDC to activate the Cs.
    • Cys is then added to the Cs solution, and the reaction takes place overnight under constant stirring.
    • The Cs-Cys product is purified through dialysis and lyophilized for future use.

    Cs-Cys-PN/PA Characterization

    • The chemical structure and composition of Cs-Cys-PN were analyzed using Fourier Transform Infrared (FT-IR) spectroscopy and 1H Nuclear Magnetic Resonance (1H NMR) spectroscopy.
    • The results of the analysis indicated that the NIPAM had been successfully coupled to Cs-Cys.
    • FT-IR spectra of Cs-Cys-PN exhibited characteristic peaks corresponding to the stretching vibrations of the amide I and amide II bands of NIPAM, C–H of NIPAM, C–– O of NIPAM, and N–H of Cs-Cys-PN.
    • 1H NMR spectra of Cs-Cys-PN showed characteristic peaks corresponding to the ring protons of Cs and the -CH of Cs-Cys, confirming the successful coupling of Cs-Cys.

    Morphological Observation of Cs-Cys-PN/PA

    • The morphology of Cs-Cys-PN/PA was studied using scanning electron microscopy (SEM).
    • SEM images revealed the morphology of Cs-Cys-PN/PA as a porous network structure with spherical particles.
    • This structure provides a large surface area for potential interactions and applications.

    Biological Activity of Cs-Cys-PN/PA

    • The study investigated the biocompatibility and antibacterial activity of Cs-Cys-PN/PA using different methods.
    • Cell viability was measured using the MTT assay, demonstrating that Cs-Cys-PN/PA exhibited good biocompatibility.
    • The antibacterial activity of Cs-Cys-PN/PA was evaluated against Staphylococcus aureus (S. aureus).
    • SEM images revealed morphological changes in S. aureus cells after treatment with Cs-Cys-PN/PA, indicating disruption of bacterial cell membranes and potential antimicrobial activity.
    • Confocal laser scanning microscopy (CLSM) was used to visualize the uptake of Cs-Cys-PN/PA by Hela cells.
    • The results showed that Cs-Cys-PN/PA was successfully internalized into the cells, suggesting potential applications in drug delivery.

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    Description

    This quiz covers the synthesis and characterization of the Cs-Cys-PN polymer, formed from chitosan, L-Cysteine, and N-isopropylacrylamide. You will explore the chemical reactions and analytical techniques used to assess the product's structure and composition. Perfect for students studying polymer chemistry and material science.

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