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COPY: CRISPR-Cas9: A Powerful Tool for Genetic Manipulation
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COPY: CRISPR-Cas9: A Powerful Tool for Genetic Manipulation

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Questions and Answers

What is the function of the CRISPR-Cas9 system in bacteria?

  • To synthesize viral DNA into the bacterial genome
  • To transcribe viral RNA into the bacterial genome
  • To cleave the viral DNA, protecting the bacteria (correct)
  • To replicate the bacterial genome
  • What is the purpose of the guide RNA in the CRISPR-Cas9 system?

  • To synthesize the target DNA sequence
  • To cleave the target DNA sequence
  • To target a specific region of DNA (correct)
  • To replicate the target DNA sequence
  • What is the result of the CRISPR-Cas9 system in human cells?

  • Transcription of viral RNA
  • Creation of viral genomes
  • Creation of point mutations, knockouts, and knock-ins (correct)
  • Replication of the human genome
  • What is the function of the Cas9 protein in the CRISPR-Cas9 system?

    <p>To cleave the target DNA sequence</p> Signup and view all the answers

    What is the purpose of agarose gel electrophoresis in the CRISPR-Cas9 system?

    <p>To analyze the results of the CRISPR-Cas9 system</p> Signup and view all the answers

    What is the abbreviation CRISPR?

    <p>Clustered Regularly Interspaced Short Palindromic Repeats</p> Signup and view all the answers

    What is the result of changing the guide RNA sequence in the CRISPR-Cas9 system?

    <p>Targeting different regions of DNA</p> Signup and view all the answers

    What type of cells can the CRISPR-Cas9 system be performed on?

    <p>Various cell types, including human cells and human stem cells</p> Signup and view all the answers

    What is the function of the CRISPR-Cas9 system in genetic manipulation?

    <p>To create point mutations, knockouts, and knock-ins</p> Signup and view all the answers

    What is the defense mechanism used by bacteria against viruses?

    <p>CRISPR-Cas9 system</p> Signup and view all the answers

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