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COPY: CRISPR-Cas9: A Powerful Tool for Genetic Manipulation

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COPY: CRISPR-Cas9: A Powerful Tool for Genetic Manipulation

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Questions and Answers

What is the function of the CRISPR-Cas9 system in bacteria?

To synthesize viral DNA into the bacterial genome

To transcribe viral RNA into the bacterial genome

To cleave the viral DNA, protecting the bacteria (correct)

To replicate the bacterial genome

What is the purpose of the guide RNA in the CRISPR-Cas9 system?

To synthesize the target DNA sequence

To cleave the target DNA sequence

To target a specific region of DNA (correct)

To replicate the target DNA sequence

What is the result of the CRISPR-Cas9 system in human cells?

Transcription of viral RNA

Creation of viral genomes

Creation of point mutations, knockouts, and knock-ins (correct)

Replication of the human genome

What is the function of the Cas9 protein in the CRISPR-Cas9 system?

To cleave the target DNA sequence (B) Signup and view all the answers

What is the purpose of agarose gel electrophoresis in the CRISPR-Cas9 system?

To analyze the results of the CRISPR-Cas9 system (B) Signup and view all the answers

What is the abbreviation CRISPR?

Clustered Regularly Interspaced Short Palindromic Repeats (C) Signup and view all the answers

What is the result of changing the guide RNA sequence in the CRISPR-Cas9 system?

Targeting different regions of DNA (C) Signup and view all the answers

What type of cells can the CRISPR-Cas9 system be performed on?

Various cell types, including human cells and human stem cells (A) Signup and view all the answers

What is the function of the CRISPR-Cas9 system in genetic manipulation?

To create point mutations, knockouts, and knock-ins (B) Signup and view all the answers

What is the defense mechanism used by bacteria against viruses?

CRISPR-Cas9 system (C) Signup and view all the answers