Podcast
Questions and Answers
What is the primary function of the Cas9 protein in the CRISPR-Cas9 technology?
What is the primary function of the Cas9 protein in the CRISPR-Cas9 technology?
- To cut viral DNA (correct)
- To produce insulin
- To transfer genes to bacterial cells
- To create guide RNA
CRISPR-Cas9 can only add new genes but cannot disable existing ones.
CRISPR-Cas9 can only add new genes but cannot disable existing ones.
False (B)
Name one medical application of recombinant DNA technology.
Name one medical application of recombinant DNA technology.
Production of human insulin (Humulin)
The __________ is a guide system that directs Cas9 to the target DNA sequence in the CRISPR-Cas9 system.
The __________ is a guide system that directs Cas9 to the target DNA sequence in the CRISPR-Cas9 system.
Which of the following statements best describes the function of recombinant DNA technology?
Which of the following statements best describes the function of recombinant DNA technology?
Match the following components with their functions in the CRISPR-Cas9 system:
Match the following components with their functions in the CRISPR-Cas9 system:
Human insulin produced through genetic modification has fewer side effects compared to insulin extracted from animals.
Human insulin produced through genetic modification has fewer side effects compared to insulin extracted from animals.
The process of producing proteins like insulin through recombinant DNA involves ___________ vats filled with bacterial cultures.
The process of producing proteins like insulin through recombinant DNA involves ___________ vats filled with bacterial cultures.
What is the primary purpose of genetic engineering?
What is the primary purpose of genetic engineering?
Recombinant DNA is created only using human DNA.
Recombinant DNA is created only using human DNA.
Name a common bacterium used in genetic engineering.
Name a common bacterium used in genetic engineering.
Restriction enzymes cut DNA at specific ________.
Restriction enzymes cut DNA at specific ________.
Match the following applications of genetic engineering with their uses:
Match the following applications of genetic engineering with their uses:
What is the correct sequence of steps in cloning a gene?
What is the correct sequence of steps in cloning a gene?
Bacterial plasmids can replicate independently within the bacterial cell.
Bacterial plasmids can replicate independently within the bacterial cell.
What is the role of bacterial plasmids in genetic engineering?
What is the role of bacterial plasmids in genetic engineering?
What is the function of primers in automated PCR?
What is the function of primers in automated PCR?
STRs are sequences that are repeated multiple times and remain constant among individuals.
STRs are sequences that are repeated multiple times and remain constant among individuals.
Which of these is NOT a potential benefit of genetically modified foods?
Which of these is NOT a potential benefit of genetically modified foods?
What does gel electrophoresis accomplish in forensic analysis?
What does gel electrophoresis accomplish in forensic analysis?
Proteomics focuses on studying the complete set of proteins in an organism.
Proteomics focuses on studying the complete set of proteins in an organism.
In DNA profiling, forensic scientists typically compare ______ STR sites.
In DNA profiling, forensic scientists typically compare ______ STR sites.
What is systems biology?
What is systems biology?
In the process of gel electrophoresis, which DNA fragments move further in the gel?
In the process of gel electrophoresis, which DNA fragments move further in the gel?
The controversy surrounding genetically modified foods often revolves around issues of ______, potential environmental impacts, and the need for mandatory labeling.
The controversy surrounding genetically modified foods often revolves around issues of ______, potential environmental impacts, and the need for mandatory labeling.
Match the following terms with their descriptions:
Match the following terms with their descriptions:
Match the following terms with their corresponding definitions:
Match the following terms with their corresponding definitions:
Describe the role of an electric field in gel electrophoresis.
Describe the role of an electric field in gel electrophoresis.
Crime scene DNA can have the same number of repeats as suspect DNA for a specific STR site.
Crime scene DNA can have the same number of repeats as suspect DNA for a specific STR site.
What does the difference in band location in gel electrophoresis indicate?
What does the difference in band location in gel electrophoresis indicate?
Earl Washington was convicted based on DNA profiling.
Earl Washington was convicted based on DNA profiling.
What is the primary purpose of DNA sequencing?
What is the primary purpose of DNA sequencing?
STR analysis has a probability of two individuals having identical DNA profiles between 1 in ____ billion and 1 in several trillion.
STR analysis has a probability of two individuals having identical DNA profiles between 1 in ____ billion and 1 in several trillion.
Which of the following cases utilized DNA profiling to exonerate an individual?
Which of the following cases utilized DNA profiling to exonerate an individual?
DNA profiling is only useful in modern forensic cases.
DNA profiling is only useful in modern forensic cases.
What is one application of bioinformatics in DNA research?
What is one application of bioinformatics in DNA research?
Which genetically modified plants produce proteins that may serve as edible vaccines?
Which genetically modified plants produce proteins that may serve as edible vaccines?
Genetically modified strawberries cannot withstand cold weather.
Genetically modified strawberries cannot withstand cold weather.
What advancement in genetically modified pigs converts less healthy fatty acids?
What advancement in genetically modified pigs converts less healthy fatty acids?
Genetically modified _______ can produce lysozyme, an antibacterial enzyme, from their milk.
Genetically modified _______ can produce lysozyme, an antibacterial enzyme, from their milk.
What is a significant advantage of genetically modified corn, soybean, and cotton?
What is a significant advantage of genetically modified corn, soybean, and cotton?
Match the genetically modified organisms with their benefits:
Match the genetically modified organisms with their benefits:
Humans cannot benefit from genetically modified organisms.
Humans cannot benefit from genetically modified organisms.
Which genetically modified animal does not have horns?
Which genetically modified animal does not have horns?
What is the primary goal of gene therapy?
What is the primary goal of gene therapy?
Short Tandem Repeat (STR) Analysis is used primarily for amplifying DNA samples.
Short Tandem Repeat (STR) Analysis is used primarily for amplifying DNA samples.
What viral technique is used in gene therapy to introduce normal genes into a patient's cells?
What viral technique is used in gene therapy to introduce normal genes into a patient's cells?
PCR stands for _____________.
PCR stands for _____________.
Match the DNA profiling techniques with their descriptions:
Match the DNA profiling techniques with their descriptions:
Which step is NOT part of a typical DNA profiling investigation?
Which step is NOT part of a typical DNA profiling investigation?
Gel Electrophoresis allows for the comparison of DNA profiles by separating fragments based on their size.
Gel Electrophoresis allows for the comparison of DNA profiles by separating fragments based on their size.
What are bone marrow cells particularly suitable for gene therapy?
What are bone marrow cells particularly suitable for gene therapy?
Which organism's genome was sequenced first in 1995?
Which organism's genome was sequenced first in 1995?
The Human Genome Project was completed in the year 2000.
The Human Genome Project was completed in the year 2000.
What is the estimated number of human genes?
What is the estimated number of human genes?
The Human Genome Project aimed to determine the nucleotide sequence of all human ______.
The Human Genome Project aimed to determine the nucleotide sequence of all human ______.
Which of the following organisms is not mentioned as having its genome sequenced?
Which of the following organisms is not mentioned as having its genome sequenced?
Genomes contain genetic information that contributes to characteristics developed during life.
Genomes contain genetic information that contributes to characteristics developed during life.
What sequencing technique is commonly used for genomes?
What sequencing technique is commonly used for genomes?
The human genome consists of 22 pairs of ______ and one pair of sex chromosomes.
The human genome consists of 22 pairs of ______ and one pair of sex chromosomes.
Match the organisms with their genome sequencing status:
Match the organisms with their genome sequencing status:
What was a significant result of the Human Genome Project?
What was a significant result of the Human Genome Project?
Which of the following concerns is raised by human gene therapy?
Which of the following concerns is raised by human gene therapy?
The Genetic Information Nondiscrimination Act of 2008 provides protection against discrimination based on genetic information in health insurance and employment.
The Genetic Information Nondiscrimination Act of 2008 provides protection against discrimination based on genetic information in health insurance and employment.
What is one potential benefit and one potential risk of creating a DNA profile for every person at birth?
What is one potential benefit and one potential risk of creating a DNA profile for every person at birth?
The Y chromosome is primarily passed from father to _____ in human ancestry.
The Y chromosome is primarily passed from father to _____ in human ancestry.
Match the historical figures to their associated genetic studies:
Match the historical figures to their associated genetic studies:
What ethical dilemma is associated with using human growth hormone (HGH) for children who are hormonally normal?
What ethical dilemma is associated with using human growth hormone (HGH) for children who are hormonally normal?
Genetic profiling can potentially lead to benefits without any concerns regarding privacy.
Genetic profiling can potentially lead to benefits without any concerns regarding privacy.
What is one reason genetic variety is deemed necessary?
What is one reason genetic variety is deemed necessary?
The practice of obtaining DNA samples from suspects before _____ was upheld by the U.S. Supreme Court in 2014.
The practice of obtaining DNA samples from suspects before _____ was upheld by the U.S. Supreme Court in 2014.
What percentage of recent Central Asian males may share genetic similarities tracing back to Genghis Khan?
What percentage of recent Central Asian males may share genetic similarities tracing back to Genghis Khan?
Flashcards
CRISPR-Cas9
CRISPR-Cas9
A gene editing technology that modifies specific genes in living cells using a guide RNA and the Cas9 enzyme.
Guide RNA
Guide RNA
A sequence of RNA that is complementary to the target DNA and directs Cas9 to the right location.
Cas9
Cas9
An enzyme that cuts DNA at a specific location identified by the guide RNA.
Recombinant DNA technology
Recombinant DNA technology
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Humulin
Humulin
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Restriction enzyme
Restriction enzyme
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DNA ligase
DNA ligase
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Gene knockout
Gene knockout
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Genetic Engineering
Genetic Engineering
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Recombinant DNA
Recombinant DNA
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Bacterial Plasmids
Bacterial Plasmids
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Gene Cloning Steps
Gene Cloning Steps
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Pest Resistance
Pest Resistance
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Medical Treatments via Cloning
Medical Treatments via Cloning
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Bioremediation
Bioremediation
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Gel electrophoresis
Gel electrophoresis
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STR Analysis
STR Analysis
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DNA profiling
DNA profiling
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Earl Washington case
Earl Washington case
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Next-generation sequencing
Next-generation sequencing
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Bioinformatics
Bioinformatics
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Identical DNA probability
Identical DNA probability
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Historical DNA cases
Historical DNA cases
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Genetically Modified Organisms (GMOs)
Genetically Modified Organisms (GMOs)
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Insect-resistance
Insect-resistance
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Golden Rice 2
Golden Rice 2
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Transgenic Animals
Transgenic Animals
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Lysozyme
Lysozyme
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Salt Tolerance
Salt Tolerance
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Vaccine Production in Plants
Vaccine Production in Plants
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Hornless Cattle
Hornless Cattle
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PCR
PCR
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Primers
Primers
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Short Tandem Repeats
Short Tandem Repeats
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STR sites
STR sites
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Forensic analysis
Forensic analysis
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Comparative STR analysis
Comparative STR analysis
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Gene Therapy
Gene Therapy
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Bone Marrow Cells
Bone Marrow Cells
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Polymerase Chain Reaction (PCR)
Polymerase Chain Reaction (PCR)
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Short Tandem Repeat (STR) Analysis
Short Tandem Repeat (STR) Analysis
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Amplification in DNA Profiling
Amplification in DNA Profiling
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DNA Isolation
DNA Isolation
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Genomics
Genomics
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Proteomics
Proteomics
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Systems Biology
Systems Biology
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Safety Concerns in DNA Technology
Safety Concerns in DNA Technology
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Whole-genome shotgun method
Whole-genome shotgun method
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Human Genome Project
Human Genome Project
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Human genome size
Human genome size
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Number of human genes
Number of human genes
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GenBank
GenBank
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Sequencing eukaryotic genomes
Sequencing eukaryotic genomes
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First sequenced genomes
First sequenced genomes
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Genome repositories
Genome repositories
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Evolution and genomics
Evolution and genomics
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HGH Treatment Ethics
HGH Treatment Ethics
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Gene Therapy Debate
Gene Therapy Debate
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Genetic Engineering in Gametes
Genetic Engineering in Gametes
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Genetic Privacy Issues
Genetic Privacy Issues
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Supreme Court on DNA Sampling
Supreme Court on DNA Sampling
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Genetic Information Nondiscrimination Act
Genetic Information Nondiscrimination Act
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Y Chromosome and Ancestry
Y Chromosome and Ancestry
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Genghis Khan's Genetics
Genghis Khan's Genetics
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Lemba People Heritage
Lemba People Heritage
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Evolution and Genetic Variety
Evolution and Genetic Variety
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Study Notes
Genetic Engineering
- Genetic engineering is the manipulation of an organism's genes using biotechnology
- Recombinant DNA technology is a type of genetic engineering
- Scientists use recombinant DNA technology to produce specific proteins
- Genetically modified organisms (GMOs) are organisms that have had their genes altered.
- Bacteria are often used as a carrier of the genes in genetic engineering.
- Plasmids, small circular DNA molecules found in bacteria, can be used in recombinant DNA technology to carry genes.
Gene Editing
- Gene editing is a technique that allows scientists to precisely change a gene within a given organism.
- CRISPR-Cas9 is one method of gene editing.
- The CRISPR-Cas9 system targets a specific DNA sequence and makes a cut in the DNA, allowing for the insertion or deletion of DNA sequences.
- Gene editing can have various applications in both medicine and biotechnology.
DNA Profiling and Forensic Science
- DNA profiling is an approach for identifying a person by examining variations in their DNA sequences.
- DNA profiling uses short tandem repeats (STRs), which are sequences of DNA bases that are repeated many times in a row, to create a DNA profile.
- Specific STRs are used as genetic markers across populations.
- DNA profiling is useful in criminal investigations and paternity testing.
- Polymerase chain reaction (PCR) is a technique for amplifying DNA to high concentrations for testing purposes.
DNA Sequencing
- DNA sequencing is a method to determine the precise order of nucleotides in a DNA molecule.
- There are several approaches for sequencing DNA, including the Sanger method and next-generation sequencing technologies.
- Next-generation sequencing is considerably faster and cheaper compared to the Sanger method.
Genetically Modified Organisms
- GMOs are organisms whose genetic material has been altered through genetic engineering.
- Common GMOs include plants (crops).
- GMOs are used to improve crop yields and enhance nutritional value.
- Concerns about GMOs relate to safety, environmental effects, and ethical considerations.
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