Genetic Engineering and CRISPR-Cas9 Quiz

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Questions and Answers

What is the primary function of the Cas9 protein in the CRISPR-Cas9 technology?

  • To cut viral DNA (correct)
  • To produce insulin
  • To transfer genes to bacterial cells
  • To create guide RNA

CRISPR-Cas9 can only add new genes but cannot disable existing ones.

False (B)

Name one medical application of recombinant DNA technology.

Production of human insulin (Humulin)

The __________ is a guide system that directs Cas9 to the target DNA sequence in the CRISPR-Cas9 system.

<p>guide RNA</p> Signup and view all the answers

Which of the following statements best describes the function of recombinant DNA technology?

<p>It enables the transfer of genes to produce proteins. (B)</p> Signup and view all the answers

Match the following components with their functions in the CRISPR-Cas9 system:

<p>Cas9 = Cuts DNA Guide RNA = Directs Cas9 to target DNA Spacer DNA = Corresponds to viral DNA sequences Repair Enzymes = Reconnects DNA strands</p> Signup and view all the answers

Human insulin produced through genetic modification has fewer side effects compared to insulin extracted from animals.

<p>True (A)</p> Signup and view all the answers

The process of producing proteins like insulin through recombinant DNA involves ___________ vats filled with bacterial cultures.

<p>fermentation</p> Signup and view all the answers

What is the primary purpose of genetic engineering?

<p>To manipulate genes for practical applications (C)</p> Signup and view all the answers

Recombinant DNA is created only using human DNA.

<p>False (B)</p> Signup and view all the answers

Name a common bacterium used in genetic engineering.

<p><em>Escherichia coli</em></p> Signup and view all the answers

Restriction enzymes cut DNA at specific ________.

<p>locations</p> Signup and view all the answers

Match the following applications of genetic engineering with their uses:

<p>Pest resistance = Increases pest resistance in plants Medical treatments = Produces proteins for disease treatment Waste cleaning = Genetically modifies bacteria for toxic waste cleanup Producing valuable chemicals = Mass-produces drugs for cancer treatment</p> Signup and view all the answers

What is the correct sequence of steps in cloning a gene?

<p>Isolate DNA, Combine DNA, Introduce into bacteria, Growth and Production (C)</p> Signup and view all the answers

Bacterial plasmids can replicate independently within the bacterial cell.

<p>True (A)</p> Signup and view all the answers

What is the role of bacterial plasmids in genetic engineering?

<p>To carry genes and replicate independently</p> Signup and view all the answers

What is the function of primers in automated PCR?

<p>To target specific DNA regions for amplification (A)</p> Signup and view all the answers

STRs are sequences that are repeated multiple times and remain constant among individuals.

<p>False (B)</p> Signup and view all the answers

Which of these is NOT a potential benefit of genetically modified foods?

<p>Guaranteed safety for all consumers (C)</p> Signup and view all the answers

What does gel electrophoresis accomplish in forensic analysis?

<p>It separates DNA fragments based on size.</p> Signup and view all the answers

Proteomics focuses on studying the complete set of proteins in an organism.

<p>True (A)</p> Signup and view all the answers

In DNA profiling, forensic scientists typically compare ______ STR sites.

<p>13</p> Signup and view all the answers

What is systems biology?

<p>Systems biology is a method of studying whole biological systems by analyzing the interactions of its parts.</p> Signup and view all the answers

In the process of gel electrophoresis, which DNA fragments move further in the gel?

<p>Smaller fragments (B)</p> Signup and view all the answers

The controversy surrounding genetically modified foods often revolves around issues of ______, potential environmental impacts, and the need for mandatory labeling.

<p>safety</p> Signup and view all the answers

Match the following terms with their descriptions:

<p>STRs = Short sequences of nucleotides that are repeated PCR = Technique used to amplify DNA Gel Electrophoresis = Process to separate DNA fragments based on size DNA Profiling = Creation of a DNA database from STR analysis</p> Signup and view all the answers

Match the following terms with their corresponding definitions:

<p>Genomics = The study of the complete set of proteins in an organism. Proteomics = The study of the complete set of genes in an organism. Systems Biology = A method of studying biological systems by analyzing the interactions of its parts.</p> Signup and view all the answers

Describe the role of an electric field in gel electrophoresis.

<p>An electric field causes negatively charged DNA fragments to move through the gel.</p> Signup and view all the answers

Crime scene DNA can have the same number of repeats as suspect DNA for a specific STR site.

<p>True (A)</p> Signup and view all the answers

What does the difference in band location in gel electrophoresis indicate?

<p>The DNA samples are from different individuals. (C)</p> Signup and view all the answers

Earl Washington was convicted based on DNA profiling.

<p>False (B)</p> Signup and view all the answers

What is the primary purpose of DNA sequencing?

<p>To determine the complete nucleotide sequence of DNA molecules.</p> Signup and view all the answers

STR analysis has a probability of two individuals having identical DNA profiles between 1 in ____ billion and 1 in several trillion.

<p>10</p> Signup and view all the answers

Which of the following cases utilized DNA profiling to exonerate an individual?

<p>Earl Washington's case (D)</p> Signup and view all the answers

DNA profiling is only useful in modern forensic cases.

<p>False (B)</p> Signup and view all the answers

What is one application of bioinformatics in DNA research?

<p>To store and analyze biological data.</p> Signup and view all the answers

Which genetically modified plants produce proteins that may serve as edible vaccines?

<p>Potatoes (D)</p> Signup and view all the answers

Genetically modified strawberries cannot withstand cold weather.

<p>False (B)</p> Signup and view all the answers

What advancement in genetically modified pigs converts less healthy fatty acids?

<p>Genes that convert less healthy fatty acids to healthier omega-3 fatty acids</p> Signup and view all the answers

Genetically modified _______ can produce lysozyme, an antibacterial enzyme, from their milk.

<p>goats</p> Signup and view all the answers

What is a significant advantage of genetically modified corn, soybean, and cotton?

<p>Insect resistance (B)</p> Signup and view all the answers

Match the genetically modified organisms with their benefits:

<p>Golden rice 2 = Helps prevent vitamin A deficiency Transgenic cattle = Increased muscle growth Salt-tolerant rice = Cultivation in harsher environments Transgenic pigs = Healthier fatty acid balance</p> Signup and view all the answers

Humans cannot benefit from genetically modified organisms.

<p>False (B)</p> Signup and view all the answers

Which genetically modified animal does not have horns?

<p>Hornless dairy cows</p> Signup and view all the answers

What is the primary goal of gene therapy?

<p>To treat diseases by introducing new genes (A)</p> Signup and view all the answers

Short Tandem Repeat (STR) Analysis is used primarily for amplifying DNA samples.

<p>False (B)</p> Signup and view all the answers

What viral technique is used in gene therapy to introduce normal genes into a patient's cells?

<p>Infecting bone marrow cells with a harmless virus</p> Signup and view all the answers

PCR stands for _____________.

<p>Polymerase Chain Reaction</p> Signup and view all the answers

Match the DNA profiling techniques with their descriptions:

<p>Polymerase Chain Reaction = Technique for amplifying specific DNA segments Short Tandem Repeat Analysis = Identifies unique patterns in repeated DNA sequences Gel Electrophoresis = Separates DNA fragments based on size DNA Isolation = Prepares samples for analysis</p> Signup and view all the answers

Which step is NOT part of a typical DNA profiling investigation?

<p>Infecting cells with a virus (D)</p> Signup and view all the answers

Gel Electrophoresis allows for the comparison of DNA profiles by separating fragments based on their size.

<p>True (A)</p> Signup and view all the answers

What are bone marrow cells particularly suitable for gene therapy?

<p>They continually multiply and produce blood cells.</p> Signup and view all the answers

Which organism's genome was sequenced first in 1995?

<p><em>Haemophilus influenzae</em> (C)</p> Signup and view all the answers

The Human Genome Project was completed in the year 2000.

<p>False (B)</p> Signup and view all the answers

What is the estimated number of human genes?

<p>21,000</p> Signup and view all the answers

The Human Genome Project aimed to determine the nucleotide sequence of all human ______.

<p>DNA</p> Signup and view all the answers

Which of the following organisms is not mentioned as having its genome sequenced?

<p>Bird (D)</p> Signup and view all the answers

Genomes contain genetic information that contributes to characteristics developed during life.

<p>True (A)</p> Signup and view all the answers

What sequencing technique is commonly used for genomes?

<p>Whole-genome shotgun method</p> Signup and view all the answers

The human genome consists of 22 pairs of ______ and one pair of sex chromosomes.

<p>autosomes</p> Signup and view all the answers

Match the organisms with their genome sequencing status:

<p><em>Haemophilus influenzae</em> = First organism sequenced <em>Arabidopsis thaliana</em> = Plant genome sequenced <em>Caenorhabditis elegans</em> = Roundworm genome sequenced <em>Drosophila melanogaster</em> = Fruit fly genome sequenced</p> Signup and view all the answers

What was a significant result of the Human Genome Project?

<p>Determined more than 99% of the human genome sequence (D)</p> Signup and view all the answers

Which of the following concerns is raised by human gene therapy?

<p>Moral implications of altering human genes (D)</p> Signup and view all the answers

The Genetic Information Nondiscrimination Act of 2008 provides protection against discrimination based on genetic information in health insurance and employment.

<p>True (A)</p> Signup and view all the answers

What is one potential benefit and one potential risk of creating a DNA profile for every person at birth?

<p>Benefit: Could link crimes to perpetrators; Risk: Sacrifices genetic privacy.</p> Signup and view all the answers

The Y chromosome is primarily passed from father to _____ in human ancestry.

<p>son</p> Signup and view all the answers

Match the historical figures to their associated genetic studies:

<p>Genghis Khan = Central Asian male ancestry Niall of the Nine Hostages = Irish male ancestry Lemba people = Ancient Jewish ancestry Genetic Information Nondiscrimination Act = Protection against genetic discrimination</p> Signup and view all the answers

What ethical dilemma is associated with using human growth hormone (HGH) for children who are hormonally normal?

<p>It may create height-based discrimination. (B)</p> Signup and view all the answers

Genetic profiling can potentially lead to benefits without any concerns regarding privacy.

<p>False (B)</p> Signup and view all the answers

What is one reason genetic variety is deemed necessary?

<p>For adaptation to changing environmental conditions.</p> Signup and view all the answers

The practice of obtaining DNA samples from suspects before _____ was upheld by the U.S. Supreme Court in 2014.

<p>conviction</p> Signup and view all the answers

What percentage of recent Central Asian males may share genetic similarities tracing back to Genghis Khan?

<p>8% (C)</p> Signup and view all the answers

Flashcards

CRISPR-Cas9

A gene editing technology that modifies specific genes in living cells using a guide RNA and the Cas9 enzyme.

Guide RNA

A sequence of RNA that is complementary to the target DNA and directs Cas9 to the right location.

Cas9

An enzyme that cuts DNA at a specific location identified by the guide RNA.

Recombinant DNA technology

A method that allows the combination of DNA from different sources to create new genetic combinations.

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Humulin

Human insulin produced by genetically modified bacteria to treat diabetes.

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Restriction enzyme

An enzyme that cuts DNA into fragments at specific sequences.

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DNA ligase

An enzyme that joins DNA fragments together by forming bonds between nucleotides.

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Gene knockout

A genetic technique to disable a gene, rendering it inactive.

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Genetic Engineering

Direct manipulation of genes for practical applications.

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Recombinant DNA

DNA created by combining DNA from different organisms.

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Bacterial Plasmids

Circular DNA molecules in bacteria used for cloning.

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Gene Cloning Steps

Four main steps: isolate DNA, combine DNA, introduce into bacteria, then grow.

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Pest Resistance

Genes inserted into plants to protect against pests.

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Medical Treatments via Cloning

Producing proteins like insulin through genetic engineering.

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Bioremediation

Using genetically modified bacteria to clean toxic waste.

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Gel electrophoresis

A technique used to separate DNA fragments based on size and charge.

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STR Analysis

Short Tandem Repeat analysis; a method for DNA profiling that examines specific regions.

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DNA profiling

A forensic technique that analyzes the unique patterns in DNA for identification purposes.

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Earl Washington case

An example where STR analysis proved innocence after wrongful conviction.

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Next-generation sequencing

A rapid and cost-effective method to sequence thousands of DNA fragments simultaneously.

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Bioinformatics

The application of computer science to store and analyze biological data.

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Identical DNA probability

The likelihood that two individuals have the same DNA profile, ranging from 1 in 10 billion to trillions.

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Historical DNA cases

Using DNA profiling to solve historical questions about ancestry and identity.

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Genetically Modified Organisms (GMOs)

Organisms whose genetic material has been altered using genetic engineering techniques.

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Insect-resistance

Genetically engineered trait in crops that allows them to resist insect pests.

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Golden Rice 2

A genetically modified rice that produces beta-carotene, aiding in preventing vitamin A deficiency.

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Transgenic Animals

Animals that have been genetically modified to express desired traits, like producing beneficial proteins.

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Lysozyme

An antibacterial enzyme produced by transgenic goats in their milk.

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Salt Tolerance

The ability of genetically modified crops, like rice, to survive in high salinity environments.

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Vaccine Production in Plants

Using genetically modified potatoes and rice to produce protein for vaccines.

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Hornless Cattle

Dairy cows genetically modified to naturally lack horns.

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PCR

Polymerase Chain Reaction, a method to amplify DNA.

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Primers

Short DNA sequences that initiate DNA replication during PCR.

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Short Tandem Repeats

Repeating sequences in DNA useful for profiling.

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STR sites

Locations in the genome where STRs are found for comparison.

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Forensic analysis

Examination of DNA evidence in a crime scene investigation.

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Comparative STR analysis

Examining different STRs to determine DNA similarity.

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Gene Therapy

A treatment method that introduces new genes to cure diseases.

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Bone Marrow Cells

Cells that continuously multiply to produce blood cells, ideal for gene therapy.

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Polymerase Chain Reaction (PCR)

A method to rapidly amplify DNA segments for analysis.

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Short Tandem Repeat (STR) Analysis

A method identifying unique short DNA sequence patterns in individuals.

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Amplification in DNA Profiling

The process of creating large DNA samples from small amounts for comparison.

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DNA Isolation

The first step in DNA profiling, extracting DNA from samples.

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Genomics

The study of the complete set of genes in an organism.

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Proteomics

The analysis of the complete set of proteins in an organism.

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Systems Biology

A method to study biological systems through interactions and parts analysis.

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Safety Concerns in DNA Technology

Concerns about the potential hazards of DNA technology, including harmful organisms.

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Whole-genome shotgun method

A technique for sequencing genomes by chopping DNA, sequencing fragments, and reassembling them.

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Human Genome Project

An international effort to sequence the entire human genome and identify all genes.

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Human genome size

Comprises 22 pairs of autosomes and one pair of sex chromosomes with 3 billion nucleotide pairs.

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Number of human genes

Estimated to be around 21,000, which is relatively low for complex organisms.

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GenBank

A database that stores DNA sequences for numerous organisms, regularly updated.

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Sequencing eukaryotic genomes

Includes sequencing genomes of protists, fungi, plants, and animals.

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First sequenced genomes

Included the bacterium Haemophilus influenzae, yeast, roundworm, and fruit fly.

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Genome repositories

Stores genetic information that influence organismal characteristics during development.

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Evolution and genomics

Genomics helps understand the evolution of life and how organisms are related.

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HGH Treatment Ethics

The moral considerations surrounding the use of human growth hormone to increase height in children.

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Gene Therapy Debate

The controversy over whether altering human genes is morally acceptable.

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Genetic Engineering in Gametes

The practice of altering sperm or ova, which raises ethical questions.

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Genetic Privacy Issues

Concerns related to the privacy of an individual's genetic information.

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Supreme Court on DNA Sampling

The 2014 ruling allowing DNA collection from suspects before conviction.

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Genetic Information Nondiscrimination Act

A law prohibiting discrimination based on genetic information in health insurance and employment.

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Y Chromosome and Ancestry

The Y chromosome is passed from father to son and reveals male ancestry.

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Genghis Khan's Genetics

Studies show common Y DNA links to Genghis Khan among Central Asian males.

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Lemba People Heritage

Y DNA studies suggest the Lemba of Africa are descendants of ancient Jews.

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Evolution and Genetic Variety

Genetic diversity is crucial for adaptation and evolution.

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Study Notes

Genetic Engineering

  • Genetic engineering is the manipulation of an organism's genes using biotechnology
  • Recombinant DNA technology is a type of genetic engineering
  • Scientists use recombinant DNA technology to produce specific proteins
  • Genetically modified organisms (GMOs) are organisms that have had their genes altered.
  • Bacteria are often used as a carrier of the genes in genetic engineering.
  • Plasmids, small circular DNA molecules found in bacteria, can be used in recombinant DNA technology to carry genes.

Gene Editing

  • Gene editing is a technique that allows scientists to precisely change a gene within a given organism.
  • CRISPR-Cas9 is one method of gene editing.
  • The CRISPR-Cas9 system targets a specific DNA sequence and makes a cut in the DNA, allowing for the insertion or deletion of DNA sequences.
  • Gene editing can have various applications in both medicine and biotechnology.

DNA Profiling and Forensic Science

  • DNA profiling is an approach for identifying a person by examining variations in their DNA sequences.
  • DNA profiling uses short tandem repeats (STRs), which are sequences of DNA bases that are repeated many times in a row, to create a DNA profile.
  • Specific STRs are used as genetic markers across populations.
  • DNA profiling is useful in criminal investigations and paternity testing.
  • Polymerase chain reaction (PCR) is a technique for amplifying DNA to high concentrations for testing purposes.

DNA Sequencing

  • DNA sequencing is a method to determine the precise order of nucleotides in a DNA molecule.
  • There are several approaches for sequencing DNA, including the Sanger method and next-generation sequencing technologies.
  • Next-generation sequencing is considerably faster and cheaper compared to the Sanger method.

Genetically Modified Organisms

  • GMOs are organisms whose genetic material has been altered through genetic engineering.
  • Common GMOs include plants (crops).
  • GMOs are used to improve crop yields and enhance nutritional value.
  • Concerns about GMOs relate to safety, environmental effects, and ethical considerations.

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