Genetic Engineering and CRISPR-Cas9 Quiz
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Questions and Answers

What is the primary function of the Cas9 protein in the CRISPR-Cas9 technology?

  • To cut viral DNA (correct)
  • To produce insulin
  • To transfer genes to bacterial cells
  • To create guide RNA
  • CRISPR-Cas9 can only add new genes but cannot disable existing ones.

    False (B)

    Name one medical application of recombinant DNA technology.

    Production of human insulin (Humulin)

    The __________ is a guide system that directs Cas9 to the target DNA sequence in the CRISPR-Cas9 system.

    <p>guide RNA</p> Signup and view all the answers

    Which of the following statements best describes the function of recombinant DNA technology?

    <p>It enables the transfer of genes to produce proteins. (B)</p> Signup and view all the answers

    Match the following components with their functions in the CRISPR-Cas9 system:

    <p>Cas9 = Cuts DNA Guide RNA = Directs Cas9 to target DNA Spacer DNA = Corresponds to viral DNA sequences Repair Enzymes = Reconnects DNA strands</p> Signup and view all the answers

    Human insulin produced through genetic modification has fewer side effects compared to insulin extracted from animals.

    <p>True (A)</p> Signup and view all the answers

    The process of producing proteins like insulin through recombinant DNA involves ___________ vats filled with bacterial cultures.

    <p>fermentation</p> Signup and view all the answers

    What is the primary purpose of genetic engineering?

    <p>To manipulate genes for practical applications (C)</p> Signup and view all the answers

    Recombinant DNA is created only using human DNA.

    <p>False (B)</p> Signup and view all the answers

    Name a common bacterium used in genetic engineering.

    <p><em>Escherichia coli</em></p> Signup and view all the answers

    Restriction enzymes cut DNA at specific ________.

    <p>locations</p> Signup and view all the answers

    Match the following applications of genetic engineering with their uses:

    <p>Pest resistance = Increases pest resistance in plants Medical treatments = Produces proteins for disease treatment Waste cleaning = Genetically modifies bacteria for toxic waste cleanup Producing valuable chemicals = Mass-produces drugs for cancer treatment</p> Signup and view all the answers

    What is the correct sequence of steps in cloning a gene?

    <p>Isolate DNA, Combine DNA, Introduce into bacteria, Growth and Production (C)</p> Signup and view all the answers

    Bacterial plasmids can replicate independently within the bacterial cell.

    <p>True (A)</p> Signup and view all the answers

    What is the role of bacterial plasmids in genetic engineering?

    <p>To carry genes and replicate independently</p> Signup and view all the answers

    What is the function of primers in automated PCR?

    <p>To target specific DNA regions for amplification (A)</p> Signup and view all the answers

    STRs are sequences that are repeated multiple times and remain constant among individuals.

    <p>False (B)</p> Signup and view all the answers

    Which of these is NOT a potential benefit of genetically modified foods?

    <p>Guaranteed safety for all consumers (C)</p> Signup and view all the answers

    What does gel electrophoresis accomplish in forensic analysis?

    <p>It separates DNA fragments based on size.</p> Signup and view all the answers

    Proteomics focuses on studying the complete set of proteins in an organism.

    <p>True (A)</p> Signup and view all the answers

    In DNA profiling, forensic scientists typically compare ______ STR sites.

    <p>13</p> Signup and view all the answers

    What is systems biology?

    <p>Systems biology is a method of studying whole biological systems by analyzing the interactions of its parts.</p> Signup and view all the answers

    In the process of gel electrophoresis, which DNA fragments move further in the gel?

    <p>Smaller fragments (B)</p> Signup and view all the answers

    The controversy surrounding genetically modified foods often revolves around issues of ______, potential environmental impacts, and the need for mandatory labeling.

    <p>safety</p> Signup and view all the answers

    Match the following terms with their descriptions:

    <p>STRs = Short sequences of nucleotides that are repeated PCR = Technique used to amplify DNA Gel Electrophoresis = Process to separate DNA fragments based on size DNA Profiling = Creation of a DNA database from STR analysis</p> Signup and view all the answers

    Match the following terms with their corresponding definitions:

    <p>Genomics = The study of the complete set of proteins in an organism. Proteomics = The study of the complete set of genes in an organism. Systems Biology = A method of studying biological systems by analyzing the interactions of its parts.</p> Signup and view all the answers

    Describe the role of an electric field in gel electrophoresis.

    <p>An electric field causes negatively charged DNA fragments to move through the gel.</p> Signup and view all the answers

    Crime scene DNA can have the same number of repeats as suspect DNA for a specific STR site.

    <p>True (A)</p> Signup and view all the answers

    What does the difference in band location in gel electrophoresis indicate?

    <p>The DNA samples are from different individuals. (C)</p> Signup and view all the answers

    Earl Washington was convicted based on DNA profiling.

    <p>False (B)</p> Signup and view all the answers

    What is the primary purpose of DNA sequencing?

    <p>To determine the complete nucleotide sequence of DNA molecules.</p> Signup and view all the answers

    STR analysis has a probability of two individuals having identical DNA profiles between 1 in ____ billion and 1 in several trillion.

    <p>10</p> Signup and view all the answers

    Which of the following cases utilized DNA profiling to exonerate an individual?

    <p>Earl Washington's case (D)</p> Signup and view all the answers

    DNA profiling is only useful in modern forensic cases.

    <p>False (B)</p> Signup and view all the answers

    What is one application of bioinformatics in DNA research?

    <p>To store and analyze biological data.</p> Signup and view all the answers

    Which genetically modified plants produce proteins that may serve as edible vaccines?

    <p>Potatoes (D)</p> Signup and view all the answers

    Genetically modified strawberries cannot withstand cold weather.

    <p>False (B)</p> Signup and view all the answers

    What advancement in genetically modified pigs converts less healthy fatty acids?

    <p>Genes that convert less healthy fatty acids to healthier omega-3 fatty acids</p> Signup and view all the answers

    Genetically modified _______ can produce lysozyme, an antibacterial enzyme, from their milk.

    <p>goats</p> Signup and view all the answers

    What is a significant advantage of genetically modified corn, soybean, and cotton?

    <p>Insect resistance (B)</p> Signup and view all the answers

    Match the genetically modified organisms with their benefits:

    <p>Golden rice 2 = Helps prevent vitamin A deficiency Transgenic cattle = Increased muscle growth Salt-tolerant rice = Cultivation in harsher environments Transgenic pigs = Healthier fatty acid balance</p> Signup and view all the answers

    Humans cannot benefit from genetically modified organisms.

    <p>False (B)</p> Signup and view all the answers

    Which genetically modified animal does not have horns?

    <p>Hornless dairy cows</p> Signup and view all the answers

    What is the primary goal of gene therapy?

    <p>To treat diseases by introducing new genes (A)</p> Signup and view all the answers

    Short Tandem Repeat (STR) Analysis is used primarily for amplifying DNA samples.

    <p>False (B)</p> Signup and view all the answers

    What viral technique is used in gene therapy to introduce normal genes into a patient's cells?

    <p>Infecting bone marrow cells with a harmless virus</p> Signup and view all the answers

    PCR stands for _____________.

    <p>Polymerase Chain Reaction</p> Signup and view all the answers

    Match the DNA profiling techniques with their descriptions:

    <p>Polymerase Chain Reaction = Technique for amplifying specific DNA segments Short Tandem Repeat Analysis = Identifies unique patterns in repeated DNA sequences Gel Electrophoresis = Separates DNA fragments based on size DNA Isolation = Prepares samples for analysis</p> Signup and view all the answers

    Which step is NOT part of a typical DNA profiling investigation?

    <p>Infecting cells with a virus (D)</p> Signup and view all the answers

    Gel Electrophoresis allows for the comparison of DNA profiles by separating fragments based on their size.

    <p>True (A)</p> Signup and view all the answers

    What are bone marrow cells particularly suitable for gene therapy?

    <p>They continually multiply and produce blood cells.</p> Signup and view all the answers

    Which organism's genome was sequenced first in 1995?

    <p><em>Haemophilus influenzae</em> (C)</p> Signup and view all the answers

    The Human Genome Project was completed in the year 2000.

    <p>False (B)</p> Signup and view all the answers

    What is the estimated number of human genes?

    <p>21,000</p> Signup and view all the answers

    The Human Genome Project aimed to determine the nucleotide sequence of all human ______.

    <p>DNA</p> Signup and view all the answers

    Which of the following organisms is not mentioned as having its genome sequenced?

    <p>Bird (D)</p> Signup and view all the answers

    Genomes contain genetic information that contributes to characteristics developed during life.

    <p>True (A)</p> Signup and view all the answers

    What sequencing technique is commonly used for genomes?

    <p>Whole-genome shotgun method</p> Signup and view all the answers

    The human genome consists of 22 pairs of ______ and one pair of sex chromosomes.

    <p>autosomes</p> Signup and view all the answers

    Match the organisms with their genome sequencing status:

    <p><em>Haemophilus influenzae</em> = First organism sequenced <em>Arabidopsis thaliana</em> = Plant genome sequenced <em>Caenorhabditis elegans</em> = Roundworm genome sequenced <em>Drosophila melanogaster</em> = Fruit fly genome sequenced</p> Signup and view all the answers

    What was a significant result of the Human Genome Project?

    <p>Determined more than 99% of the human genome sequence (D)</p> Signup and view all the answers

    Which of the following concerns is raised by human gene therapy?

    <p>Moral implications of altering human genes (D)</p> Signup and view all the answers

    The Genetic Information Nondiscrimination Act of 2008 provides protection against discrimination based on genetic information in health insurance and employment.

    <p>True (A)</p> Signup and view all the answers

    What is one potential benefit and one potential risk of creating a DNA profile for every person at birth?

    <p>Benefit: Could link crimes to perpetrators; Risk: Sacrifices genetic privacy.</p> Signup and view all the answers

    The Y chromosome is primarily passed from father to _____ in human ancestry.

    <p>son</p> Signup and view all the answers

    Match the historical figures to their associated genetic studies:

    <p>Genghis Khan = Central Asian male ancestry Niall of the Nine Hostages = Irish male ancestry Lemba people = Ancient Jewish ancestry Genetic Information Nondiscrimination Act = Protection against genetic discrimination</p> Signup and view all the answers

    What ethical dilemma is associated with using human growth hormone (HGH) for children who are hormonally normal?

    <p>It may create height-based discrimination. (B)</p> Signup and view all the answers

    Genetic profiling can potentially lead to benefits without any concerns regarding privacy.

    <p>False (B)</p> Signup and view all the answers

    What is one reason genetic variety is deemed necessary?

    <p>For adaptation to changing environmental conditions.</p> Signup and view all the answers

    The practice of obtaining DNA samples from suspects before _____ was upheld by the U.S. Supreme Court in 2014.

    <p>conviction</p> Signup and view all the answers

    What percentage of recent Central Asian males may share genetic similarities tracing back to Genghis Khan?

    <p>8% (C)</p> Signup and view all the answers

    Flashcards

    CRISPR-Cas9

    A gene editing technology that modifies specific genes in living cells using a guide RNA and the Cas9 enzyme.

    Guide RNA

    A sequence of RNA that is complementary to the target DNA and directs Cas9 to the right location.

    Cas9

    An enzyme that cuts DNA at a specific location identified by the guide RNA.

    Recombinant DNA technology

    A method that allows the combination of DNA from different sources to create new genetic combinations.

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    Humulin

    Human insulin produced by genetically modified bacteria to treat diabetes.

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    Restriction enzyme

    An enzyme that cuts DNA into fragments at specific sequences.

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    DNA ligase

    An enzyme that joins DNA fragments together by forming bonds between nucleotides.

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    Gene knockout

    A genetic technique to disable a gene, rendering it inactive.

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    Genetic Engineering

    Direct manipulation of genes for practical applications.

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    Recombinant DNA

    DNA created by combining DNA from different organisms.

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    Bacterial Plasmids

    Circular DNA molecules in bacteria used for cloning.

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    Gene Cloning Steps

    Four main steps: isolate DNA, combine DNA, introduce into bacteria, then grow.

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    Pest Resistance

    Genes inserted into plants to protect against pests.

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    Medical Treatments via Cloning

    Producing proteins like insulin through genetic engineering.

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    Bioremediation

    Using genetically modified bacteria to clean toxic waste.

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    Gel electrophoresis

    A technique used to separate DNA fragments based on size and charge.

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    STR Analysis

    Short Tandem Repeat analysis; a method for DNA profiling that examines specific regions.

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    DNA profiling

    A forensic technique that analyzes the unique patterns in DNA for identification purposes.

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    Earl Washington case

    An example where STR analysis proved innocence after wrongful conviction.

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    Next-generation sequencing

    A rapid and cost-effective method to sequence thousands of DNA fragments simultaneously.

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    Bioinformatics

    The application of computer science to store and analyze biological data.

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    Identical DNA probability

    The likelihood that two individuals have the same DNA profile, ranging from 1 in 10 billion to trillions.

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    Historical DNA cases

    Using DNA profiling to solve historical questions about ancestry and identity.

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    Genetically Modified Organisms (GMOs)

    Organisms whose genetic material has been altered using genetic engineering techniques.

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    Insect-resistance

    Genetically engineered trait in crops that allows them to resist insect pests.

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    Golden Rice 2

    A genetically modified rice that produces beta-carotene, aiding in preventing vitamin A deficiency.

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    Transgenic Animals

    Animals that have been genetically modified to express desired traits, like producing beneficial proteins.

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    Lysozyme

    An antibacterial enzyme produced by transgenic goats in their milk.

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    Salt Tolerance

    The ability of genetically modified crops, like rice, to survive in high salinity environments.

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    Vaccine Production in Plants

    Using genetically modified potatoes and rice to produce protein for vaccines.

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    Hornless Cattle

    Dairy cows genetically modified to naturally lack horns.

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    PCR

    Polymerase Chain Reaction, a method to amplify DNA.

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    Primers

    Short DNA sequences that initiate DNA replication during PCR.

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    Short Tandem Repeats

    Repeating sequences in DNA useful for profiling.

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    STR sites

    Locations in the genome where STRs are found for comparison.

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    Forensic analysis

    Examination of DNA evidence in a crime scene investigation.

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    Comparative STR analysis

    Examining different STRs to determine DNA similarity.

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    Gene Therapy

    A treatment method that introduces new genes to cure diseases.

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    Bone Marrow Cells

    Cells that continuously multiply to produce blood cells, ideal for gene therapy.

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    Polymerase Chain Reaction (PCR)

    A method to rapidly amplify DNA segments for analysis.

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    Short Tandem Repeat (STR) Analysis

    A method identifying unique short DNA sequence patterns in individuals.

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    Amplification in DNA Profiling

    The process of creating large DNA samples from small amounts for comparison.

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    DNA Isolation

    The first step in DNA profiling, extracting DNA from samples.

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    Genomics

    The study of the complete set of genes in an organism.

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    Proteomics

    The analysis of the complete set of proteins in an organism.

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    Systems Biology

    A method to study biological systems through interactions and parts analysis.

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    Safety Concerns in DNA Technology

    Concerns about the potential hazards of DNA technology, including harmful organisms.

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    Whole-genome shotgun method

    A technique for sequencing genomes by chopping DNA, sequencing fragments, and reassembling them.

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    Human Genome Project

    An international effort to sequence the entire human genome and identify all genes.

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    Human genome size

    Comprises 22 pairs of autosomes and one pair of sex chromosomes with 3 billion nucleotide pairs.

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    Number of human genes

    Estimated to be around 21,000, which is relatively low for complex organisms.

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    GenBank

    A database that stores DNA sequences for numerous organisms, regularly updated.

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    Sequencing eukaryotic genomes

    Includes sequencing genomes of protists, fungi, plants, and animals.

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    First sequenced genomes

    Included the bacterium Haemophilus influenzae, yeast, roundworm, and fruit fly.

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    Genome repositories

    Stores genetic information that influence organismal characteristics during development.

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    Evolution and genomics

    Genomics helps understand the evolution of life and how organisms are related.

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    HGH Treatment Ethics

    The moral considerations surrounding the use of human growth hormone to increase height in children.

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    Gene Therapy Debate

    The controversy over whether altering human genes is morally acceptable.

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    Genetic Engineering in Gametes

    The practice of altering sperm or ova, which raises ethical questions.

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    Genetic Privacy Issues

    Concerns related to the privacy of an individual's genetic information.

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    Supreme Court on DNA Sampling

    The 2014 ruling allowing DNA collection from suspects before conviction.

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    Genetic Information Nondiscrimination Act

    A law prohibiting discrimination based on genetic information in health insurance and employment.

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    Y Chromosome and Ancestry

    The Y chromosome is passed from father to son and reveals male ancestry.

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    Genghis Khan's Genetics

    Studies show common Y DNA links to Genghis Khan among Central Asian males.

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    Lemba People Heritage

    Y DNA studies suggest the Lemba of Africa are descendants of ancient Jews.

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    Evolution and Genetic Variety

    Genetic diversity is crucial for adaptation and evolution.

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    Study Notes

    Genetic Engineering

    • Genetic engineering is the manipulation of an organism's genes using biotechnology
    • Recombinant DNA technology is a type of genetic engineering
    • Scientists use recombinant DNA technology to produce specific proteins
    • Genetically modified organisms (GMOs) are organisms that have had their genes altered.
    • Bacteria are often used as a carrier of the genes in genetic engineering.
    • Plasmids, small circular DNA molecules found in bacteria, can be used in recombinant DNA technology to carry genes.

    Gene Editing

    • Gene editing is a technique that allows scientists to precisely change a gene within a given organism.
    • CRISPR-Cas9 is one method of gene editing.
    • The CRISPR-Cas9 system targets a specific DNA sequence and makes a cut in the DNA, allowing for the insertion or deletion of DNA sequences.
    • Gene editing can have various applications in both medicine and biotechnology.

    DNA Profiling and Forensic Science

    • DNA profiling is an approach for identifying a person by examining variations in their DNA sequences.
    • DNA profiling uses short tandem repeats (STRs), which are sequences of DNA bases that are repeated many times in a row, to create a DNA profile.
    • Specific STRs are used as genetic markers across populations.
    • DNA profiling is useful in criminal investigations and paternity testing.
    • Polymerase chain reaction (PCR) is a technique for amplifying DNA to high concentrations for testing purposes.

    DNA Sequencing

    • DNA sequencing is a method to determine the precise order of nucleotides in a DNA molecule.
    • There are several approaches for sequencing DNA, including the Sanger method and next-generation sequencing technologies.
    • Next-generation sequencing is considerably faster and cheaper compared to the Sanger method.

    Genetically Modified Organisms

    • GMOs are organisms whose genetic material has been altered through genetic engineering.
    • Common GMOs include plants (crops).
    • GMOs are used to improve crop yields and enhance nutritional value.
    • Concerns about GMOs relate to safety, environmental effects, and ethical considerations.

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    Description

    Test your knowledge on the key concepts of genetic engineering and CRISPR-Cas9 technology. This quiz covers the functions of the Cas9 protein, applications of recombinant DNA technology, and the mechanisms behind gene editing. Explore how these advancements are shaping medicine and biotechnology.

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