Column Chromatography Phases and Properties

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What is the role of the stationary phase in column chromatography?

To provide good adsorption properties and allow free flow of the mobile phase

Which factor is NOT a condition for the stationary phase in column chromatography?

Ability to react with acids and bases

What is the function of the mobile phase in column chromatography?

To act as a developing agent

Which solvent is commonly used as a mobile phase in column chromatography?

Ethanol

What is one of the requirements for particles in the stationary phase of column chromatography?

Uniform shape and size in the range of 60 – 200µ

What is the function of an eluting agent in column chromatography?

To facilitate the removal of separated components from the column

The stationary phase in column chromatography should have particles with a diameter range of 60 – 200µ.

True

The stationary phase should be chemically reactive with acids and bases for effective separation in column chromatography.

False

The mobile phase in column chromatography consists of solid materials with good adsorption properties.

False

Ethanol and water are examples of solvents commonly used in the mobile phase of column chromatography.

True

The stationary phase should allow restricted flow of the mobile phase for better separation in column chromatography.

False

The mobile phase acts as a developing agent in column chromatography to form bands of separated components.

True

Pyridine is an example of a solid stationary phase used in column chromatography.

False

The stationary phase should have high mechanical stability and be chemically reactive to ensure effective separation in column chromatography.

False

The mobile phase aids in removing the separated components from the column in column chromatography.

True

In column chromatography, the stationary phase should be colorful and expensive to ensure quality separation.

False

Learn about the different phases involved in column chromatography, such as the stationary phase and its properties like shape, size, stability, and inertness. Understand the importance of these factors in separating mixtures effectively.

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