Column Chromatography Phases and Properties
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Questions and Answers

What is the role of the stationary phase in column chromatography?

  • To act as a solvent-sample mixture
  • To remove components from the column
  • To help in the separation of components in the sample to form bands
  • To provide good adsorption properties and allow free flow of the mobile phase (correct)

    • Which factor is NOT a condition for the stationary phase in column chromatography?

  • Uniform shape and size
  • Ability to react with acids and bases (correct)
  • Stability and inertness
  • Readily available and colorless

    • What is the function of the mobile phase in column chromatography?

  • To act as a developing agent (correct)
  • To provide good adsorption properties to the column
  • To act as an eluting agent
  • To allow free flow of the stationary phase

    • Which solvent is commonly used as a mobile phase in column chromatography?

    <p>Ethanol</p> Signup and view all the answers

    What is one of the requirements for particles in the stationary phase of column chromatography?

    <p>Uniform shape and size in the range of 60 – 200µ</p> Signup and view all the answers

    What is the function of an eluting agent in column chromatography?

    <p>To facilitate the removal of separated components from the column</p> Signup and view all the answers

    The stationary phase in column chromatography should have particles with a diameter range of 60 – 200µ.

    <p>True</p> Signup and view all the answers

    The stationary phase should be chemically reactive with acids and bases for effective separation in column chromatography.

    <p>False</p> Signup and view all the answers

    The mobile phase in column chromatography consists of solid materials with good adsorption properties.

    <p>False</p> Signup and view all the answers

    Ethanol and water are examples of solvents commonly used in the mobile phase of column chromatography.

    <p>True</p> Signup and view all the answers

    The stationary phase should allow restricted flow of the mobile phase for better separation in column chromatography.

    <p>False</p> Signup and view all the answers

    The mobile phase acts as a developing agent in column chromatography to form bands of separated components.

    <p>True</p> Signup and view all the answers

    Pyridine is an example of a solid stationary phase used in column chromatography.

    <p>False</p> Signup and view all the answers

    The stationary phase should have high mechanical stability and be chemically reactive to ensure effective separation in column chromatography.

    <p>False</p> Signup and view all the answers

    The mobile phase aids in removing the separated components from the column in column chromatography.

    <p>True</p> Signup and view all the answers

    In column chromatography, the stationary phase should be colorful and expensive to ensure quality separation.

    <p>False</p> Signup and view all the answers

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