Column Chromatography Phases and Properties

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Questions and Answers

What is the role of the stationary phase in column chromatography?

  • To act as a solvent-sample mixture
  • To remove components from the column
  • To help in the separation of components in the sample to form bands
  • To provide good adsorption properties and allow free flow of the mobile phase (correct)

Which factor is NOT a condition for the stationary phase in column chromatography?

  • Uniform shape and size
  • Ability to react with acids and bases (correct)
  • Stability and inertness
  • Readily available and colorless

What is the function of the mobile phase in column chromatography?

  • To act as a developing agent (correct)
  • To provide good adsorption properties to the column
  • To act as an eluting agent
  • To allow free flow of the stationary phase

Which solvent is commonly used as a mobile phase in column chromatography?

<p>Ethanol (A)</p> Signup and view all the answers

What is one of the requirements for particles in the stationary phase of column chromatography?

<p>Uniform shape and size in the range of 60 – 200µ (A)</p> Signup and view all the answers

What is the function of an eluting agent in column chromatography?

<p>To facilitate the removal of separated components from the column (C)</p> Signup and view all the answers

The stationary phase in column chromatography should have particles with a diameter range of 60 – 200µ.

<p>True (A)</p> Signup and view all the answers

The stationary phase should be chemically reactive with acids and bases for effective separation in column chromatography.

<p>False (B)</p> Signup and view all the answers

The mobile phase in column chromatography consists of solid materials with good adsorption properties.

<p>False (B)</p> Signup and view all the answers

Ethanol and water are examples of solvents commonly used in the mobile phase of column chromatography.

<p>True (A)</p> Signup and view all the answers

The stationary phase should allow restricted flow of the mobile phase for better separation in column chromatography.

<p>False (B)</p> Signup and view all the answers

The mobile phase acts as a developing agent in column chromatography to form bands of separated components.

<p>True (A)</p> Signup and view all the answers

Pyridine is an example of a solid stationary phase used in column chromatography.

<p>False (B)</p> Signup and view all the answers

The stationary phase should have high mechanical stability and be chemically reactive to ensure effective separation in column chromatography.

<p>False (B)</p> Signup and view all the answers

The mobile phase aids in removing the separated components from the column in column chromatography.

<p>True (A)</p> Signup and view all the answers

In column chromatography, the stationary phase should be colorful and expensive to ensure quality separation.

<p>False (B)</p> Signup and view all the answers

Flashcards

Role of Stationary Phase in Column Chromatography

The stationary phase in column chromatography provides good adsorption properties and allows the free flow of the mobile phase.

Stationary Phase Reactivity (Column Chromatography)

The stationary phase should NOT be chemically reactive with acids and bases for effective separation.

Mobile Phase Function (Column Chromatography)

The mobile phase acts as a developing agent in column chromatography by moving the components through the stationary phase.

Common Mobile Phase Solvent

Ethanol is a common solvent used as the mobile phase in column chromatography.

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Stationary Phase Particle Size

Stationary phase particles in column chromatography typically range from 60 - 200µm.

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Function of an Elution Agent

The eluting agent facilitates the removal of separated components from the column in column chromatography.

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Stationary Phase Particle Size Range Truth

True. The stationary phase in column chromatography should have uniform particles in the 60-200µ range for effective separation.

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Stationary Phase Reactivity with Acids/Bases

False. The stationary phase should NOT be chemically reactive with acids and bases for proper separation.

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Mobile Phase Composition

False. The mobile phase in column chromatography is typically a liquid, not a solid.

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Mobile Phase Examples

True. Ethanol and water are examples of common solvents used as the mobile phase in column chromatography.

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Stationary Phase's Effect on Mobile Phase Flow

False. The stationary phase should allow free flow of the mobile phase for optimal separation.

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Mobile Phase as Developing Agent

True. The mobile phase acts as a developing agent, moving the components through the stationary phase to form bands.

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Stationary Phase Material Example

False. Pyridine is a liquid, not a solid, and therefore is not an example of a stationary phase in column chromatography.

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Stationary Phase Mechanical Stability Requirement

False. The stationary phase should have good mechanical stability, but NOT be chemically reactive.

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Mobile Phase Role in Component Removal

True. The mobile phase helps remove the separated components from the column in column chromatography.

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Stationary Phase Appearance Requirement

False. The stationary phase's appearance (color, cost) is not a factor in its effectiveness.

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