Types of Chromatography and Column Chromatography Phases

Column chromatography can be classified based on mobile phase, stationary phase, forces of separation, or method of separation. Separation a Mixture of Two Components using Column Chromatography. Different phases involved in the Column Chromatography: Before starting with the Column Chromatography Experiment let us understand the different phases involved: Stationary phase is a ______ material which should have good adsorption properties and meet specific conditions.

Stationary phase in column chromatography should have particles with a uniform shape and size in the range of 60 – 200µ in diameter. It should possess high ______ stability and be chemically inert.

The stationary phase in column chromatography should be colorless, inexpensive, and readily available. It should allow free flow of the ______ phase.

The mobile phase in column chromatography is made up of ______ and performs various functions in the separation process.

The mobile phase acts as a solvent-sample mixture that can be introduced in the column. It acts as a developing agent that helps in the separation of components in the sample to form ______.

Some examples of solvents used as mobile phases based on their polarity are ethanol, acetone, water, acetic acid, pyridine, etc. The mobile phase acts as an ______ agent where the separated components are removed from the column.

In column chromatography, the stationary phase is usually filled with ______ powder

The mobile phase in column chromatography is used to saturate the ______ powder

Column chromatography involves continuously pouring solvent into the top while opening the tap at the ______ of the column

In Thin Layer Chromatography (TLC), the stationary phase is a thin layer supported by an ______ backing

TLC is widely used due to its simplicity, low cost, high sensitivity, and speed of ______

The goal of Thin Layer Chromatography (TLC) is to obtain well-defined, well-separated ______

Chromatography is an analytical technique used for the separation, purification, identification, and quantification of components of a mixture between ______ and mobile phases.

A physical method of separation that distributes components to separate them between two phases, one ______ (stationary phase), the other is mobile (the mobile phase).

The fundamental principle of chromatographic separation is the change of the physical properties of the mixture under investigation between the mobile and ______ layers.

The basic principle of all types of chromatography is: 'Relative affinities of components of the mixture toward mobile phase and ______ phase.'

Differentiate between ______ and mobile phase: Stationary phase It is fixed and does not move.

Mobile phase travel over or through the ______ phase. Mostly the mobile phase is liquid.