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Questions and Answers
In column chromatography, the stationary phase is made up of a solid material.
In column chromatography, the stationary phase is made up of a solid material.
True (A)
Particles in the stationary phase should have a range of diameter between 60 - 200mm.
Particles in the stationary phase should have a range of diameter between 60 - 200mm.
False (B)
The stationary phase should react with acids or bases during the experiment in column chromatography.
The stationary phase should react with acids or bases during the experiment in column chromatography.
False (B)
The mobile phase in column chromatography consists of solvents only.
The mobile phase in column chromatography consists of solvents only.
Mobile phases used in column chromatography can include ethanol, acetone, water, and ammonia.
Mobile phases used in column chromatography can include ethanol, acetone, water, and ammonia.
Column chromatography involves steps like developing and eluting agents.
Column chromatography involves steps like developing and eluting agents.
Flashcards
Column Chromatography Stationary Phase
Column Chromatography Stationary Phase
A solid material in column chromatography that acts as a barrier
Stationary Phase Particle Size Range
Stationary Phase Particle Size Range
The ideal particle size range for the stationary phase in column chromatography is typically between 2 to 60 microns.
Stationary Phase Reactivity
Stationary Phase Reactivity
The stationary phase should ideally not react with acids or bases to prevent unwanted interactions during separations.
Column Chromatography Mobile Phase
Column Chromatography Mobile Phase
The mobile phase in column chromatography usually consists of a solvent(s) pushing the components through the column and is the moving part
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Mobile Phase Composition
Mobile Phase Composition
A mobile phase in column chromatography can contain solvents, such as water, ethanol, and acetone, but not strictly only solvents.
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Column Chromatography Steps
Column Chromatography Steps
Column chromatography procedures typically involve steps like developing (moving the mixture) as well as eluting(removing the mixture) the sample.
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Column Chromatography Overview
- In column chromatography, the stationary phase consists of solid material that facilitates separation.
- Particles within the stationary phase typically have diameters ranging from 60 to 200 mm, promoting effective interactions with the substances being separated.
- The stationary phase is reactive, interacting with acids or bases to enhance the separation process.
Mobile Phase Characteristics
- The mobile phase comprises only solvents, which transport the substances through the column.
- Common solvents used in the mobile phase include ethanol, acetone, water, and ammonia, each chosen based on the properties of the substances being separated.
Key Steps in the Process
- Column chromatography involves crucial steps such as developing and eluting agents, which are essential for maintaining the efficiency of the chromatographic process.
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