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Questions and Answers
In column chromatography, the stationary phase is made up of a solid material.
In column chromatography, the stationary phase is made up of a solid material.
True
Particles in the stationary phase should have a range of diameter between 60 - 200mm.
Particles in the stationary phase should have a range of diameter between 60 - 200mm.
False
The stationary phase should react with acids or bases during the experiment in column chromatography.
The stationary phase should react with acids or bases during the experiment in column chromatography.
False
The mobile phase in column chromatography consists of solvents only.
The mobile phase in column chromatography consists of solvents only.
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Mobile phases used in column chromatography can include ethanol, acetone, water, and ammonia.
Mobile phases used in column chromatography can include ethanol, acetone, water, and ammonia.
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Column chromatography involves steps like developing and eluting agents.
Column chromatography involves steps like developing and eluting agents.
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Study Notes
Column Chromatography Overview
- In column chromatography, the stationary phase consists of solid material that facilitates separation.
- Particles within the stationary phase typically have diameters ranging from 60 to 200 mm, promoting effective interactions with the substances being separated.
- The stationary phase is reactive, interacting with acids or bases to enhance the separation process.
Mobile Phase Characteristics
- The mobile phase comprises only solvents, which transport the substances through the column.
- Common solvents used in the mobile phase include ethanol, acetone, water, and ammonia, each chosen based on the properties of the substances being separated.
Key Steps in the Process
- Column chromatography involves crucial steps such as developing and eluting agents, which are essential for maintaining the efficiency of the chromatographic process.
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Description
Learn about the different phases involved in column chromatography, including the stationary phase criteria. Understand the importance of shape, size, stability, and inertness of solid materials for successful separation of mixtures.
