Column Chromatography Techniques Quiz
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Questions and Answers

What is the stationary phase in column chromatography?

  • Solvent reservoir
  • Pure silica or polymer
  • Chemically bonded support particles (correct)
  • Porous glass plate

    • In which type of chromatography does the stationary phase consist of small-diameter particles?

  • High Performance Liquid Chromatography (HPLC) (correct)
  • Gas chromatography
  • Liquid Chromatography (LC)
  • Low-performance liquid chromatography

    • What are the factors affecting column efficiency in column chromatography?

  • Dimension of the column
  • Particle size of adsorbent
  • Nature of solvent
  • All of the above (correct)

    • Which type of liquid chromatography uses large, non-rigid support material particles greater than 40 µm in diameter?

    <p>Low-performance liquid chromatography</p> Signup and view all the answers

    What is the detection method used in low-performance liquid chromatography?

    <p>Fraction collection with later analysis of each fraction</p> Signup and view all the answers

    What are the advantages of low-performance liquid chromatography?

    <p>Simple system requirements and low cost</p> Signup and view all the answers

    What is the main difference between traditional column chromatography and HPLC?

    <p>HPLC allows the sample to be applied using a closed system, while traditional column chromatography does not.</p> Signup and view all the answers

    What contributes to the high resolving power and short analysis time characteristic of HPLC?

    <p>Increased flow rates achieved by applying higher pressure and using adsorbents of small particle size.</p> Signup and view all the answers

    What are the advantages of HPLC for preparative chromatography?

    <p>Ease of automation and fast analysis time.</p> Signup and view all the answers

    In which application is HPLC used for the analysis of antibiotics?

    <p>Pharmaceutical quality control</p> Signup and view all the answers

    What determines the retention and elution of solutes in HPLC?

    <p>Interactions of solutes with both the mobile and stationary phases.</p> Signup and view all the answers

    What type of solvent would be considered a strong mobile phase for a column containing a polar stationary phase?

    <p>Polar solvent</p> Signup and view all the answers

    What is an application of HPLC in forensics?

    <p>Identification of anabolic steroids in serum, urine, sweat, and hair</p> Signup and view all the answers

    What is the nature of support material particles used in HPLC methods?

    <p>Small, uniform, rigid support material particles</p> Signup and view all the answers

    What is used to force the mobile phase through the HPLC column under high pressure?

    <p>Injection valve</p> Signup and view all the answers

    What are the reasons for the limited use of high-performance liquid chromatography (HPLC)?

    <p>Low efficiency, long analysis times, and poor limits of detection</p> Signup and view all the answers

    The stationary phase in column chromatography may be pure silica, polymer, or coated onto support particles.

    <p>True</p> Signup and view all the answers

    High Performance Liquid Chromatography (HPLC) uses large, non-rigid support material particles greater than 40 µm in diameter.

    <p>False</p> Signup and view all the answers

    Low-performance liquid chromatography (LC) methods can only tolerate high operating pressures.

    <p>False</p> Signup and view all the answers

    The nature of the solvent does not affect the efficiency of the column in column chromatography.

    <p>False</p> Signup and view all the answers

    In HPLC, the sample is usually applied directly to the bottom of the column.

    <p>False</p> Signup and view all the answers

    The detection method used in low-performance liquid chromatography involves direct analysis of each fraction without fraction collection.

    <p>False</p> Signup and view all the answers

    High-performance liquid chromatography (HPLC) is commonly used due to its high efficiency and short analysis times.

    <p>False</p> Signup and view all the answers

    HPLC uses a solvent (mobile phase) that is allowed to drip through the column under gravity.

    <p>False</p> Signup and view all the answers

    The parameters used to describe a HPLC column refer to the nature, type, and size of its stationary phase material, and the dimensions of the column used.

    <p>False</p> Signup and view all the answers

    Increased flow rates in HPLC are obtained by applying a pressure across the stationary phase.

    <p>False</p> Signup and view all the answers

    Preparative chromatography using HPLC is not suitable for automation.

    <p>False</p> Signup and view all the answers

    HPLC is not the preferred choice for purification work.

    <p>False</p> Signup and view all the answers

    HPLC is not used in pharmaceutical applications for shelf life determination of pharmaceutical products.

    <p>False</p> Signup and view all the answers

    The interactions of solutes with the stationary phase have no effect on the retention and elution of solutes in HPLC.

    <p>False</p> Signup and view all the answers

    A polar solvent would be considered a weak mobile phase for a column containing a polar stationary phase.

    <p>True</p> Signup and view all the answers

    Rapid elution in a few minutes for all compounds in the mixture is characteristic of a weak mobile phase.

    <p>False</p> Signup and view all the answers

    Study Notes

    Column Chromatography

    • The stationary phase in column chromatography can be pure silica, polymer, or coated onto support particles.

    Types of Chromatography

    • High Performance Liquid Chromatography (HPLC) uses small-diameter particles as the stationary phase.
    • Low-performance liquid chromatography (LC) methods use large, non-rigid support material particles greater than 40 µm in diameter.

    Factors Affecting Column Efficiency

    • Multiple factors affect column efficiency in column chromatography.

    Detection Methods

    • Low-performance liquid chromatography (LC) uses direct analysis of each fraction without fraction collection.

    Advantages of HPLC

    • HPLC has high efficiency and short analysis times due to its ability to use high pressures and small particles.
    • HPLC is suitable for preparative chromatography and automation.
    • HPLC is commonly used in pharmaceutical applications for shelf life determination of pharmaceutical products.

    HPLC Characteristics

    • HPLC uses a high-pressure pump to force the mobile phase through the column.
    • The interactions of solutes with the stationary phase determine the retention and elution of solutes in HPLC.
    • A non-polar solvent would be considered a strong mobile phase for a column containing a polar stationary phase.
    • Rapid elution in a few minutes for all compounds in the mixture is characteristic of a strong mobile phase.

    Applications of HPLC

    • HPLC is used in forensics and pharmaceutical applications.
    • HPLC is used for the analysis of antibiotics.

    Limitations of HPLC

    • High-performance liquid chromatography (HPLC) has limited use due to several reasons.

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    Description

    Test your knowledge of column chromatography, a separation technique used in chemistry. This quiz covers the stationary and mobile phases, different types of column chromatography such as gas chromatography and liquid chromatography, and factors affecting column efficiency.

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