18 Questions
What is the purpose of the stationary phase in column chromatography?
To separate the components of a mixture
In column chromatography, what is the role of the mobile phase?
To carry the sample through the silica powder
What is the main goal of Thin Layer Chromatography (TLC)?
To obtain well-defined, well-separated spots
What is the purpose of pouring solvent into the top of the column in column chromatography?
To carry the sample through the silica powder
Why is Thin Layer Chromatography (TLC) considered an analytical tool widely used?
Due to its simplicity and relatively low cost
What determines the speed at which a compound migrates in TLC?
Different affinities for mobile and stationary phases
What is the main function of the mobile phase in chromatography?
Separating the components in the mixture
In column chromatography, how does the separation of components occur?
By the distribution of analyte between mobile and stationary phases
What type of phase mainly remains fixed and does not move in chromatography?
Solid phase
Which type of chromatography technique commonly uses a liquid mobile phase?
Thin layer chromatography (TLC)
How can you distinguish between the stationary and mobile phases in chromatography?
Stationary phase does not move, while mobile phase travels over or through it
What is the key factor driving separation in chromatographic techniques?
The physical properties of the mixture
What is the role of the mobile phase in column chromatography?
Acts as a developing agent
Which of the following characteristics should the stationary phase possess in column chromatography?
Uniform shape and size particles
What is the primary function of the eluting agent in column chromatography?
Removing separated components from the column
In thin layer chromatography (TLC), what is analogous to the stationary phase in column chromatography?
Thin layer of adsorbent material
What role does the mobile phase play in thin layer chromatography (TLC)?
Develops bands of separated components
Which feature distinguishes column chromatography from other separation techniques?
Utilization of a mobile phase
Learn about the different types of chromatography and the phases involved in column chromatography. Explore the classification based on mobile phase, stationary phase, forces of separation, and method of separation.
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