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Types of Chromatography and Column Chromatography Phases

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Types of Chromatography and Column Chromatography Phases

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Questions and Answers

What is the purpose of the stationary phase in column chromatography?

To support the stationary phase using an inert backing
To monitor the progress of a reaction
To carry the sample through the silica powder
To separate the components of a mixture (correct)

In column chromatography, what is the role of the mobile phase?

To fill the column with silica powder
To carry the sample through the silica powder (correct)
To monitor the progress of a reaction
To obtain well-defined, well-separated spots

What is the main goal of Thin Layer Chromatography (TLC)?

To obtain well-defined, well-separated spots (correct)
To monitor the progress of a reaction
To separate the components of a mixture
To purify small amounts of a compound

What is the purpose of pouring solvent into the top of the column in column chromatography?

To carry the sample through the silica powder (D) Signup and view all the answers

Why is Thin Layer Chromatography (TLC) considered an analytical tool widely used?

Due to its simplicity and relatively low cost (C) Signup and view all the answers

What determines the speed at which a compound migrates in TLC?

Different affinities for mobile and stationary phases (D) Signup and view all the answers

What is the main function of the mobile phase in chromatography?

Separating the components in the mixture (B) Signup and view all the answers

In column chromatography, how does the separation of components occur?

By the distribution of analyte between mobile and stationary phases (B) Signup and view all the answers

What type of phase mainly remains fixed and does not move in chromatography?

Solid phase (C) Signup and view all the answers

Which type of chromatography technique commonly uses a liquid mobile phase?

Thin layer chromatography (TLC) (B) Signup and view all the answers

How can you distinguish between the stationary and mobile phases in chromatography?

Stationary phase does not move, while mobile phase travels over or through it (C) Signup and view all the answers

What is the key factor driving separation in chromatographic techniques?

The physical properties of the mixture (A) Signup and view all the answers

What is the role of the mobile phase in column chromatography?

Acts as a developing agent (D) Signup and view all the answers

Which of the following characteristics should the stationary phase possess in column chromatography?

Uniform shape and size particles (D) Signup and view all the answers

What is the primary function of the eluting agent in column chromatography?

Removing separated components from the column (A) Signup and view all the answers

In thin layer chromatography (TLC), what is analogous to the stationary phase in column chromatography?

Thin layer of adsorbent material (A) Signup and view all the answers

What role does the mobile phase play in thin layer chromatography (TLC)?

Develops bands of separated components (A) Signup and view all the answers

Which feature distinguishes column chromatography from other separation techniques?

Utilization of a mobile phase (C) Signup and view all the answers