Types of Chromatography and Column Chromatography Phases
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Questions and Answers

What is the purpose of the stationary phase in column chromatography?

  • To support the stationary phase using an inert backing
  • To monitor the progress of a reaction
  • To carry the sample through the silica powder
  • To separate the components of a mixture (correct)

    • In column chromatography, what is the role of the mobile phase?

  • To fill the column with silica powder
  • To carry the sample through the silica powder (correct)
  • To monitor the progress of a reaction
  • To obtain well-defined, well-separated spots

    • What is the main goal of Thin Layer Chromatography (TLC)?

  • To obtain well-defined, well-separated spots (correct)
  • To monitor the progress of a reaction
  • To separate the components of a mixture
  • To purify small amounts of a compound

    • What is the purpose of pouring solvent into the top of the column in column chromatography?

    <p>To carry the sample through the silica powder</p> Signup and view all the answers

    Why is Thin Layer Chromatography (TLC) considered an analytical tool widely used?

    <p>Due to its simplicity and relatively low cost</p> Signup and view all the answers

    What determines the speed at which a compound migrates in TLC?

    <p>Different affinities for mobile and stationary phases</p> Signup and view all the answers

    What is the main function of the mobile phase in chromatography?

    <p>Separating the components in the mixture</p> Signup and view all the answers

    In column chromatography, how does the separation of components occur?

    <p>By the distribution of analyte between mobile and stationary phases</p> Signup and view all the answers

    What type of phase mainly remains fixed and does not move in chromatography?

    <p>Solid phase</p> Signup and view all the answers

    Which type of chromatography technique commonly uses a liquid mobile phase?

    <p>Thin layer chromatography (TLC)</p> Signup and view all the answers

    How can you distinguish between the stationary and mobile phases in chromatography?

    <p>Stationary phase does not move, while mobile phase travels over or through it</p> Signup and view all the answers

    What is the key factor driving separation in chromatographic techniques?

    <p>The physical properties of the mixture</p> Signup and view all the answers

    What is the role of the mobile phase in column chromatography?

    <p>Acts as a developing agent</p> Signup and view all the answers

    Which of the following characteristics should the stationary phase possess in column chromatography?

    <p>Uniform shape and size particles</p> Signup and view all the answers

    What is the primary function of the eluting agent in column chromatography?

    <p>Removing separated components from the column</p> Signup and view all the answers

    In thin layer chromatography (TLC), what is analogous to the stationary phase in column chromatography?

    <p>Thin layer of adsorbent material</p> Signup and view all the answers

    What role does the mobile phase play in thin layer chromatography (TLC)?

    <p>Develops bands of separated components</p> Signup and view all the answers

    Which feature distinguishes column chromatography from other separation techniques?

    <p>Utilization of a mobile phase</p> Signup and view all the answers

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