18 Questions
What is the primary factor that influences the choice of a cell disruption method for large-scale cell disruption?
Product stability and ease of extraction from cell debris
What is the purpose of adding liquid nitrogen during cryogenic bead milling?
To reduce the temperature during processing of thermolabile materials
What is the primary advantage of using a bead mill for cell disruption?
It can be used for disrupting a wide range of cells, including yeast and animal tissue
What is the effect of increasing the rate of agitation during bead milling?
It increases the rate of cell disruption
What is the potential drawback of using bead milling for cell disruption?
It can generate enormous amounts of heat
What is the equation that describes the process of bead milling?
First Order ln(Rm/R) = kt
What is the primary objective of cell disruption in biotechnology?
To solubilize the maximum amount of product while maintaining biological activity
Which of the following is a potential detrimental effect of cell disruption?
Denaturation and oxidation of biological products
What is the primary function of the cell wall in cell disruption?
To convey strength to the cell and resist disruption
Which of the following organisms has been tested for high-pressure cell disruption?
Saccharomyces cerevisiae
What is the term for biological products that are released into the medium surrounding the cell?
Extracellular products
What is the term for biological products that are located between the inner and outer cell membranes?
Periplasmic products
What is the primary mechanism of cell disruption in a high pressure homogenizer?
Turbulence and cavitation
What is the typical operating pressure range for a high pressure homogenizer?
400-600 bar
What is the purpose of the valve/orifice design in a high pressure homogenizer?
To optimize cell disruption efficiency
What is the effect of temperature on the release rate constant (k) in high pressure homogenizers?
It increases the release rate constant
What determines the residence time in a centrifuge?
The flow rate of the sample
What is the primary factor affecting the sedimentation velocity of particles in a centrifuge?
The size of the particles
This quiz covers cell disruption methods, types of cells, and differences between Gram positive and Gram negative bacteria. It also explores the factors that influence the choice of disruption method.
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