Podcast
Questions and Answers
What is the primary factor that influences the choice of a cell disruption method for large-scale cell disruption?
What is the primary factor that influences the choice of a cell disruption method for large-scale cell disruption?
- Product stability and ease of extraction from cell debris (correct)
- Type of cell to be disrupted
- Cost of the method
- Cell membrane structure
What is the purpose of adding liquid nitrogen during cryogenic bead milling?
What is the purpose of adding liquid nitrogen during cryogenic bead milling?
- To increase the cell concentration
- To reduce the temperature during processing of thermolabile materials (correct)
- To reduce the bead diameter
- To increase the rate of agitation
What is the primary advantage of using a bead mill for cell disruption?
What is the primary advantage of using a bead mill for cell disruption?
- It can be used for disrupting a wide range of cells, including yeast and animal tissue (correct)
- It is a fast method
- It is a gentle method that does not generate heat
- It is a cost-effective method
What is the effect of increasing the rate of agitation during bead milling?
What is the effect of increasing the rate of agitation during bead milling?
What is the potential drawback of using bead milling for cell disruption?
What is the potential drawback of using bead milling for cell disruption?
What is the equation that describes the process of bead milling?
What is the equation that describes the process of bead milling?
What is the primary objective of cell disruption in biotechnology?
What is the primary objective of cell disruption in biotechnology?
Which of the following is a potential detrimental effect of cell disruption?
Which of the following is a potential detrimental effect of cell disruption?
What is the primary function of the cell wall in cell disruption?
What is the primary function of the cell wall in cell disruption?
Which of the following organisms has been tested for high-pressure cell disruption?
Which of the following organisms has been tested for high-pressure cell disruption?
What is the term for biological products that are released into the medium surrounding the cell?
What is the term for biological products that are released into the medium surrounding the cell?
What is the term for biological products that are located between the inner and outer cell membranes?
What is the term for biological products that are located between the inner and outer cell membranes?
What is the primary mechanism of cell disruption in a high pressure homogenizer?
What is the primary mechanism of cell disruption in a high pressure homogenizer?
What is the typical operating pressure range for a high pressure homogenizer?
What is the typical operating pressure range for a high pressure homogenizer?
What is the purpose of the valve/orifice design in a high pressure homogenizer?
What is the purpose of the valve/orifice design in a high pressure homogenizer?
What is the effect of temperature on the release rate constant (k) in high pressure homogenizers?
What is the effect of temperature on the release rate constant (k) in high pressure homogenizers?
What determines the residence time in a centrifuge?
What determines the residence time in a centrifuge?
What is the primary factor affecting the sedimentation velocity of particles in a centrifuge?
What is the primary factor affecting the sedimentation velocity of particles in a centrifuge?
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