Cell Disruption and Recombinant DNA
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Questions and Answers

What is the primary factor that influences the choice of a cell disruption method for large-scale cell disruption?

  • Product stability and ease of extraction from cell debris (correct)
  • Type of cell to be disrupted
  • Cost of the method
  • Cell membrane structure

    • What is the purpose of adding liquid nitrogen during cryogenic bead milling?

  • To increase the cell concentration
  • To reduce the temperature during processing of thermolabile materials (correct)
  • To reduce the bead diameter
  • To increase the rate of agitation

    • What is the primary advantage of using a bead mill for cell disruption?

  • It can be used for disrupting a wide range of cells, including yeast and animal tissue (correct)
  • It is a fast method
  • It is a gentle method that does not generate heat
  • It is a cost-effective method

    • What is the effect of increasing the rate of agitation during bead milling?

    <p>It increases the rate of cell disruption</p> Signup and view all the answers

    What is the potential drawback of using bead milling for cell disruption?

    <p>It can generate enormous amounts of heat</p> Signup and view all the answers

    What is the equation that describes the process of bead milling?

    <p>First Order ln(Rm/R) = kt</p> Signup and view all the answers

    What is the primary objective of cell disruption in biotechnology?

    <p>To solubilize the maximum amount of product while maintaining biological activity</p> Signup and view all the answers

    Which of the following is a potential detrimental effect of cell disruption?

    <p>Denaturation and oxidation of biological products</p> Signup and view all the answers

    What is the primary function of the cell wall in cell disruption?

    <p>To convey strength to the cell and resist disruption</p> Signup and view all the answers

    Which of the following organisms has been tested for high-pressure cell disruption?

    <p>Saccharomyces cerevisiae</p> Signup and view all the answers

    What is the term for biological products that are released into the medium surrounding the cell?

    <p>Extracellular products</p> Signup and view all the answers

    What is the term for biological products that are located between the inner and outer cell membranes?

    <p>Periplasmic products</p> Signup and view all the answers

    What is the primary mechanism of cell disruption in a high pressure homogenizer?

    <p>Turbulence and cavitation</p> Signup and view all the answers

    What is the typical operating pressure range for a high pressure homogenizer?

    <p>400-600 bar</p> Signup and view all the answers

    What is the purpose of the valve/orifice design in a high pressure homogenizer?

    <p>To optimize cell disruption efficiency</p> Signup and view all the answers

    What is the effect of temperature on the release rate constant (k) in high pressure homogenizers?

    <p>It increases the release rate constant</p> Signup and view all the answers

    What determines the residence time in a centrifuge?

    <p>The flow rate of the sample</p> Signup and view all the answers

    What is the primary factor affecting the sedimentation velocity of particles in a centrifuge?

    <p>The size of the particles</p> Signup and view all the answers

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