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Questions and Answers
What is the purpose of using ion exchange in the context of fermentation broths?
What is the purpose of using ion exchange in the context of fermentation broths?
- To induce precipitation by adding salts or solvents
- To recover proteins or antibiotics
- To eliminate trace biomolecules or impurities (correct)
- To separate biomolecules using affinity adsorption
Which of the following is NOT a component of an ion exchange resin used in purification processes?
Which of the following is NOT a component of an ion exchange resin used in purification processes?
- Ionic functional groups
- Polymeric network
- Counterions
- Enzyme inhibitors (correct)
What is the main chemical interaction involved in affinity adsorption for purification purposes?
What is the main chemical interaction involved in affinity adsorption for purification purposes?
- Van der Waals forces between solute and carrier particles
- Ionic bonds between solute and a ligand on carrier particles (correct)
- Covalent bonds between solute and carrier particles
- Hydrogen bonding between solute and carrier particles
Which method is widely used for the recovery of proteins or antibiotics through precipitation?
Which method is widely used for the recovery of proteins or antibiotics through precipitation?
What effect does the addition of salts have on protein solubility during precipitation?
What effect does the addition of salts have on protein solubility during precipitation?
Which organic solvent can be used to precipitate proteins by decreasing their dielectric constant?
Which organic solvent can be used to precipitate proteins by decreasing their dielectric constant?
What makes it challenging to remove proteins precipitated using ammonium sulfate?
What makes it challenging to remove proteins precipitated using ammonium sulfate?
At what percentage must sodium sulfate be used for adequate solubility when precipitating proteins?
At what percentage must sodium sulfate be used for adequate solubility when precipitating proteins?
Which component of an ion exchange resin has ionic functional groups permanently attached to it?
Which component of an ion exchange resin has ionic functional groups permanently attached to it?
What role do counterions play in an ion exchange resin?
What role do counterions play in an ion exchange resin?
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