10 Questions
What is the purpose of using ion exchange in the context of fermentation broths?
To eliminate trace biomolecules or impurities
Which of the following is NOT a component of an ion exchange resin used in purification processes?
Enzyme inhibitors
What is the main chemical interaction involved in affinity adsorption for purification purposes?
Ionic bonds between solute and a ligand on carrier particles
Which method is widely used for the recovery of proteins or antibiotics through precipitation?
Chromatography
What effect does the addition of salts have on protein solubility during precipitation?
Decreases protein solubility
Which organic solvent can be used to precipitate proteins by decreasing their dielectric constant?
Acetone
What makes it challenging to remove proteins precipitated using ammonium sulfate?
Difficulty in eliminating ammonium sulfate
At what percentage must sodium sulfate be used for adequate solubility when precipitating proteins?
~35-40%
Which component of an ion exchange resin has ionic functional groups permanently attached to it?
Polymeric network
What role do counterions play in an ion exchange resin?
Assist in separating biomolecules based on charge differences
This quiz covers effective cell disruption methods used in laboratory settings, including chemical methods involving detergents, alkalis, and organic solvents, as well as biological methods such as enzymatic digestion. It also touches on the recovery process of products from dilute aqueous solutions.
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