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Cell Disruption Methods in Laboratory

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Questions and Answers

What is the purpose of using ion exchange in the context of fermentation broths?

To induce precipitation by adding salts or solvents

To recover proteins or antibiotics

To eliminate trace biomolecules or impurities (correct)

To separate biomolecules using affinity adsorption

Which of the following is NOT a component of an ion exchange resin used in purification processes?

Ionic functional groups

Polymeric network

Counterions

Enzyme inhibitors (correct)

What is the main chemical interaction involved in affinity adsorption for purification purposes?

Van der Waals forces between solute and carrier particles

Ionic bonds between solute and a ligand on carrier particles (correct)

Covalent bonds between solute and carrier particles

Hydrogen bonding between solute and carrier particles

Which method is widely used for the recovery of proteins or antibiotics through precipitation?

Chromatography Signup and view all the answers

What effect does the addition of salts have on protein solubility during precipitation?

Decreases protein solubility Signup and view all the answers

Which organic solvent can be used to precipitate proteins by decreasing their dielectric constant?

Acetone Signup and view all the answers

What makes it challenging to remove proteins precipitated using ammonium sulfate?

Difficulty in eliminating ammonium sulfate Signup and view all the answers

At what percentage must sodium sulfate be used for adequate solubility when precipitating proteins?

~35-40% Signup and view all the answers

Which component of an ion exchange resin has ionic functional groups permanently attached to it?

Polymeric network Signup and view all the answers

What role do counterions play in an ion exchange resin?

Assist in separating biomolecules based on charge differences Signup and view all the answers