Cell Biology: Lysosomes and Mitochondria Quiz

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Questions and Answers

Which cell type is known to have the highest number of lysosomes?

  • Muscle cells
  • Nerve cells
  • White blood cells (correct)
  • Red blood cells

Red blood cells contain lysosomes.

False (B)

What is the function of the Mannose-6-phosphate signal in lysosomal enzyme processing?

It targets the enzymes for a lysosomal receptor.

Lysosomal enzymes are synthesized in the __________.

<p>rough ER</p> Signup and view all the answers

Match the following components with their role in lysosome function:

<p>Lysosomal enzymes = Digestion of waste products Golgi apparatus = Processing and packaging enzymes Mannose-6-phosphate = Targeting enzymes for lysosomes Lysosome vesicles = Fusing with waste products</p> Signup and view all the answers

What is the primary function of intermediate filaments?

<p>Provide mechanical resistance to stretch and deformation (B)</p> Signup and view all the answers

Intermediate filaments are composed of only one type of protein.

<p>False (B)</p> Signup and view all the answers

Name one of the cell types that contains keratin as an intermediate filament.

<p>Epithelial cells</p> Signup and view all the answers

Vimentin is primarily associated with __________ support and motility in many cell types.

<p>structure</p> Signup and view all the answers

Match the intermediate filament type with its main function or associated cell type:

<p>Keratin = Epithelial cells Vimentin = Many cells Desmin = Muscle contraction Cytokeratin = Mechanical resistance in epithelial tissues</p> Signup and view all the answers

What is the primary function of mitochondria in the cell?

<p>ATP synthesis (C)</p> Signup and view all the answers

Mitochondria have a single membrane structure.

<p>False (B)</p> Signup and view all the answers

What structure in mitochondria houses the electron transport chain?

<p>Inner membrane</p> Signup and view all the answers

Mitochondria are involved in _________ oxidative phosphorylation and ATP synthesis.

<p>fatty acid</p> Signup and view all the answers

Match the following organelles with their primary functions:

<p>Mitochondria = ATP synthesis Lysosome = Digestion of waste Peroxisome = Fatty acid oxidation Chloroplast = Photosynthesis</p> Signup and view all the answers

Which of the following organelles is NOT a part of the endomembrane system?

<p>Mitochondria (C)</p> Signup and view all the answers

Peroxisomes are involved in the digestion of cellular waste.

<p>False (B)</p> Signup and view all the answers

Name one characteristic feature of peroxisomes.

<p>Fatty acid oxidation</p> Signup and view all the answers

What is the primary function of cilia in the respiratory passageways?

<p>To sweep mucus and particles out of the airways (C)</p> Signup and view all the answers

The paralysis of cilia can lead to an increased risk of infections in smokers.

<p>True (A)</p> Signup and view all the answers

What health problems are associated with the failure of cilia due to smoking?

<p>Infections, inflammation, or cancer</p> Signup and view all the answers

When cilia no longer beat, _______ and __________ from tobacco use stay in the airways.

<p>pathogens, chemicals</p> Signup and view all the answers

Match the following respiratory problems with their causes related to smoking:

<p>Infections = Failure of cilia to clear pathogens Inflammation = Accumulation of chemicals Cancer = Continuous exposure to harmful substances in smoke Chronic cough = Efforts to expel retained mucus and chemicals</p> Signup and view all the answers

What is the typical cough experienced by smokers primarily trying to accomplish?

<p>To expel mucus and chemicals (B)</p> Signup and view all the answers

Cilia also help in the absorption of nutrients in the respiratory system.

<p>False (B)</p> Signup and view all the answers

Describe the impact of cigarette smoking on the respiratory cilia.

<p>Cigarette smoking paralyzes the cilia, preventing them from effectively clearing mucus and particles from the airways.</p> Signup and view all the answers

What is the primary function of lysosomes?

<p>Uptake and digestion of materials (C)</p> Signup and view all the answers

Lysosomes are involved in the breakdown of large organic molecules into smaller subunits.

<p>True (A)</p> Signup and view all the answers

Name one lysosomal disorder caused by deficiencies of lysosomal enzymes.

<p>Fabry disease</p> Signup and view all the answers

The process of _____ involves the engulfment of large regions of cytoplasm.

<p>autophagy</p> Signup and view all the answers

Match the following lysosomal disorders with their associated substances:

<p>Fabry disease = Sphingolipids Pompe disease = Glycogen Tay-Sachs disease = Sphingolipids Hurler syndrome = Proteoglycans</p> Signup and view all the answers

Which of the following substances can be broken down by lysosomes?

<p>All of the above (D)</p> Signup and view all the answers

Liver cells do not require many lysosomes for their functions.

<p>False (B)</p> Signup and view all the answers

What role do lysosomes play in single-celled organisms during starvation?

<p>Defense mechanism</p> Signup and view all the answers

What is the average pH of the inner membrane of mitochondria?

<p>8 (C)</p> Signup and view all the answers

Human oocytes contain approximately 5,000 mitochondria.

<p>False (B)</p> Signup and view all the answers

Which process involves the transfer of electrons and pumping H+ ions into the intermembrane space?

<p>Mitochondrial ATP synthesis</p> Signup and view all the answers

The ____ are the powerhouses of the cell, responsible for ATP production.

<p>mitochondria</p> Signup and view all the answers

Match each type of cell with its mitochondrial content:

<p>Red blood cells = No mitochondria Heart muscle cells = Approximately 5,000 mitochondria Human oocytes = 100,000-600,000 mitochondria</p> Signup and view all the answers

Which component of the mitochondrial structure is involved in ATP synthesis?

<p>ATP synthase (A)</p> Signup and view all the answers

Mitochondria undergo a process called biogenesis.

<p>True (A)</p> Signup and view all the answers

What is heteroplasmy in the context of mitochondria?

<p>The presence of more than one type of mitochondrial DNA within a cell.</p> Signup and view all the answers

Mitochondrial replacement therapy is associated with the concept of a ____ parent baby.

<p>three</p> Signup and view all the answers

Which of the following is NOT a function of mitochondria?

<p>Hormone secretion (D)</p> Signup and view all the answers

Flashcards

Mitochondria

The powerhouse of the cell, responsible for generating ATP through oxidative phosphorylation.

Oxidative Phosphorylation

A process within mitochondria where energy from food is converted into ATP, the cell's energy currency.

Krebs Cycle

A series of chemical reactions that occurs in the mitochondria, producing ATP and releasing electrons.

Cristae

Specialized folds in the inner mitochondrial membrane that increase the surface area for ATP production.

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Porins

A type of membrane protein found in the outer mitochondrial membrane that allows the passage of small molecules.

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Mitochondrial DNA

Small circular DNA molecules found within mitochondria, similar to bacterial chromosomes.

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Mitochondrial Biogenesis

The process of creating new mitochondria from existing ones.

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Peroxisomes

Specialized organelles that break down long-chain fatty acids, produce hydrogen peroxide as a byproduct, and play a role in detoxification.

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Why do red blood cells lack lysosomes?

Red blood cells lack lysosomes, organelles responsible for breaking down waste.

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Which cell type has the highest number of lysosomes?

White blood cells contain a high number of lysosomes to break down debris and pathogens during immune responses.

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What are lysosomes?

Lysosomes are small, membrane-bound organelles that contain enzymes for breaking down waste materials, cellular debris, and pathogens.

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Where are lysosomal enzymes synthesized?

Lysosomal enzymes are synthesized in the rough endoplasmic reticulum (ER) and then transported to the Golgi apparatus for processing.

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What is the role of mannose-6-phosphate (M-6-P) in lysosome synthesis?

A mannose-6-phosphate (M-6-P) signal is added to carbohydrate chains of lysosomal enzymes, targeting them for a specific receptor in the Golgi apparatus.

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ATP synthase

A specialized protein complex embedded in the inner membrane of mitochondria involved in ATP synthesis, which is the primary energy currency of the cell. This complex acts like a tiny motor, harnessing the energy from the proton gradient to produce ATP.

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Electron Transport Chain

A process that occurs during cellular respiration where electrons are transferred from NADH and FADH2 to oxygen, generating a proton gradient across the inner mitochondrial membrane. This gradient is crucial for ATP synthesis.

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Proton Gradient

The difference in proton concentration between the intermembrane space (high concentration) and the mitochondrial matrix (low concentration), which drives ATP synthesis. It represents the energy stored within the proton gradient.

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Electron Carriers (NADH and FADH2)

A specialized protein complex in the inner membrane of mitochondria that facilitates the transfer of electrons from NADH and FADH2 to oxygen, creating the proton gradient essential for ATP synthesis. It is a major player in the electron transport chain.

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Heteroplasmy

The presence of two or more genetically distinct mitochondrial DNAs (mtDNA) in a single cell or individual. It can occur due to mutations in mtDNA or the inheritance of different mtDNA from parents.

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Mitochondrion-Related Diseases

A medical condition that arises from mutations in mitochondrial DNA (mtDNA) affecting the function of mitochondria. These mutations can lead to a variety of symptoms, including muscle weakness, fatigue, and neurological disorders.

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Mitochondrial Replacement Therapy (MRT)

A technique used to prevent the transmission of mitochondrial diseases from mother to child. It involves replacing the mother's affected mitochondria with healthy ones from a donor, creating a three-parent embryo.

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Leigh Syndrome

A rare genetic disorder caused by mutations in mitochondrial DNA (mtDNA), resulting in severe neurological impairments, including brain abnormalities and muscle weakness. It is a type of mitochondrial disease.

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Keratin

A type of intermediate filament protein found in epithelial cells, responsible for supporting cells, resisting mechanical stress, and aiding in cell adhesion and movement.

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Desmin

A type of intermediate filament protein found in muscle cells, specifically in the muscle tissue that helps with contraction and movement.

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Vimentin

A type of intermediate filament protein found in many cell types, playing a role in cellular structure, movement, and anchoring cells to the extracellular matrix.

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Neurofilament

A type of intermediate filament protein found in nerve cells, playing an essential role in maintaining the stability of the nervous system.

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Intermediate Filaments

Strong, fibrous structures found within cells, providing structural support, mechanical resistance, and maintaining cell shape.

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What are lysosomes and what do they do?

Lysosomes are organelles that break down various materials such as worn-out organelles, bacteria, and misfolded proteins through hydrolysis.

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What is autophagy?

The process by which lysosomes engulf and breakdown large regions of cytoplasm, including organelles and other cellular components.

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What are lysosomal storage disorders?

Lysosomal storage diseases are genetic disorders caused by deficiencies in lysosomal enzymes, leading to the accumulation of undigested materials in cells.

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List some examples of lysosomal storage diseases.

Fabry disease, Gaucher disease, Tay-Sachs disease, Krabbe disease, Pompe disease, Hurler syndrome, Hunter syndrome, and Sanfilippo syndrome.

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What materials do lysosomes break down?

Lysosomes break down food particles, bacteria, worn-out organelles, misfolded proteins, and debris.

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How do lysosomes break down materials?

Lysosomes break down large molecules into smaller subunits through hydrolysis, releasing them into the cytoplasm for the cell to use.

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Why do liver cells have many lysosomes?

Liver cells have many lysosomes to break down cholesterol and other materials.

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How are lysosomes involved in the defense of single-celled organisms?

Lysosomes are involved in the defense of single-celled organisms against starvation, by breaking down cellular components for energy.

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Cilia

Tiny hair-like structures that line the respiratory passageways, moving mucus and particles upward to be expelled.

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Mucociliary Escalator

The process of moving mucus and trapped particles upward in the respiratory tract.

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Ciliary Paralysis

The loss of functional cilia in the respiratory tract, often caused by smoking.

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Smoker's Cough

A persistent and forceful cough often experienced by smokers, usually caused by the buildup of mucus due to impaired ciliary function.

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Pathogens

Microscopic organisms that can cause disease.

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Inflammation

The process of damaging or destroying cells, tissues, or organs.

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Cancer

An uncontrolled growth of abnormal cells that can invade and spread to other parts of the body.

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Detoxification

The process of removing foreign substances from the body, often by the lungs and immune system.

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Study Notes

Cell Structure 2

  • The endomembrane system includes the ER, Golgi, and endosomes
  • The digestion center includes lysosomes, peroxisomes, and vacuoles
  • The power plan includes mitochondria and chloroplasts
  • The headquarters is the nucleus

Learning Objectives

  • Mitochondria characteristics and ATP synthesis, biogenesis
  • Peroxisome characteristics
  • Lysosomes characteristics
  • Proteasomes characteristics and role of ubiquitin
  • Components of the cytoskeleton (microtubules, actin filaments, intermediate filaments)
  • Molecular motors and role in cell functions
  • Cilia and flagella structure and function

Mitochondria

  • Power plant of the cell, involved in fatty acid oxidation and ATP synthesis
  • Two membranes: outer (perforated with porins) and inner (cristae with electron transport chain)
  • Contain bacterial-type chromosomes (no introns)
  • 103-104 mitochondria in mammalian cells
  • pH ~ 8

Mitochondrial ATP Synthesis

  • Electrons transported from NADH and FADH2 to oxygen, pumping H+ ions into intermembrane space
  • H+ gradient powers ATP synthesis by ATP synthase
  • ATP synthase rotary pump uses energy for ATP production

Mitochondrial Biogenesis

  • Nuclear genes on nuclear DNA code for over 1,000 mitochondrial proteins synthesized in the cytoplasm
  • Mitochondrial genes on mitochondrial DNA code for 13 mitochondrial membrane proteins, 22 tRNAs, and 2 rRNAs
  • Proteins imported into mitochondria via specific import mechanisms
  • Visual diagrams and processes of the import system

Digestion Center (Lysosomes)

  • 50-1000 lysosomes per cell
  • pH ~4.8 (acidic)
  • Contain digestive enzymes produced by the rough ER
  • Digest proteins, glycogen, sphingolipids, etc.
  • Prevent self-digestion by maintaining acidic internal environment and membrane integrity

Lysosome Function

  • Digest food particles, bacteria, and cellular waste
  • Break down complex organic molecules into smaller subunits
  • Recycle cellular components; crucial for cells

Peroxisomes

  • Surrounded by a single membrane
  • Contain around 50 enzymes imported from the cytoplasm
  • pH ~6.9-7.1
  • Oxidize 25% of the ethanol alcohol humans drink

Peroxisome functions

  • Oxidize toxic substances (e.g., H2O2) for elimination.
  • Break down fatty acids
  • Synthesize plasmalogen phospholipids—crucial components of some cell membranes.

Lysosomal Disorders

  • Deficiencies in lysosomal enzymes cause rare genetic disorders (e.g., Fabry, Gaucher, Tay-Sachs, Pompe).
  • Accumulation of undegraded substances leads to cellular damage.

Cytoskeleton

  • The cytoskeleton is the internal scaffolding of the cell, providing support and mechanical strength.
  • Components include microtubules, actin filaments, and intermediate filaments.
  • Microfilaments, intermediate filaments, and microtubules each have different dimensions, proteins, and distinct roles and functions.

Cytoskeleton Function

  • Cell shape, internal organization, intracellular transport, cell assembly into tissues, and cell movement

Actin Filaments (Microfilaments)

  • Consist of actin protein monomers.
  • Involved in cell shape changes, contractile rings during cell division, and muscle contraction
  • Polymerization and depolymerization enables motility

Intermediate Filaments

  • Strong, flexible fibers, providing cell structural support, resistance to stretch, and mechanical stress.
  • Consist of various proteins (Keratin, Vimentin, Lamin).
  • Various functions based on cellular type (e.g. maintaining cell shape and strength in epithelial cells, supporting cellular structures in muscle cells)

Microtubules

  • Hollow tubes composed of α- and β-tubulin dimers.
  • Involved in intracellular transport, maintaining cell shape, and forming the basis for cilia and flagella.
  • Motor proteins (dynein and kinesin) move along microtubules to transport cargo, such as vesicles and organelles.

Centrosomes

  • Microtubule-organizing centers.
  • Consist of two centrioles arranged at right angles.
  • Organize microtubules and form the mitotic spindle during cell division.
  • Form the basal bodies that anchor cilia and flagella

Cilia and Flagella

  • Motile cellular extensions composed of microtubules.
  • Drive currents of fluids across the cell surface.
  • Used for movement of substances and in some cases, the cell itself.
  • Composed of a distinctive 9+2 structural arrangement of microtubules.

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