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Questions and Answers
What is cell biology?
What is cell biology?
The science which deals with the structure, function, and molecular construction of the cell.
What are the two main procedures for studying cells?
What are the two main procedures for studying cells?
- Examination of fixed tissues (correct)
- Study of cell environments
- Examination of dead cells
- Examination of living cells (correct)
What is tissue culture?
What is tissue culture?
The maintenance and study of live cells and tissues outside the body in culture (in vitro).
What is a common example of a fixative?
What is a common example of a fixative?
The process of _____ means the gradual extraction of water from the tissue.
The process of _____ means the gradual extraction of water from the tissue.
What is the purpose of embedding fixed tissues in paraffin?
What is the purpose of embedding fixed tissues in paraffin?
What is the purpose of staining in tissue preparation?
What is the purpose of staining in tissue preparation?
Staining involves using organic solvents to increase the clarity of tissues.
Staining involves using organic solvents to increase the clarity of tissues.
Flashcards
Cell Culture
Cell Culture
Maintenance and study of living cells and tissues outside the body, in vitro.
Tissue Culture
Tissue Culture
A specific type of cell culture focusing on tissues rather than isolated cells.
In Vitro
In Vitro
Experiments performed outside a living organism, typically in a lab.
In Vivo
In Vivo
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Paraffin Sections
Paraffin Sections
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Fixation
Fixation
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Dehydration
Dehydration
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Clearing
Clearing
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Embedding
Embedding
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Staining
Staining
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Study Notes
Cell Biology
- Cell biology is the study of cell structure, function, and molecular construction.
- Cytology is synonymous with cell biology
- Methods for cell study include examining living cells (tissue culture) and fixed tissues.
Methods of Studying Cells
- Tissue Culture:
- Maintains and studies cells and tissues outside the body.
- Allows direct observation of living cells.
- Enables experiments not possible in the body (in vivo).
- Uses include research on cell structure and function, and genetic/chromosomal analysis.
- Fixed Tissue Examination:
- Uses paraffin, frozen, or plastic sections.
Preparation of Paraffin Sections
- Obtain fresh tissue, a few millimeters thick.
- Fixation: Preserve tissues to give them a lifelike state.
- Examples of fixatives include 10% formalin, Bouin's solution, osmic acid, and 4% glutaraldehyde + 10% formalin.
- Dehydration: Gradually remove water from tissue using increasing concentrations of ethanol.
- Clearing: Replace alcohol with a solvent like xylol to clear tissue.
- Impregnation: Soak the tissue in melted paraffin.
- Embedding: Embed the tissue in solidified paraffin, which supports the structure for slicing.
- Cutting: Cut the specimen into thin slices.
- Mounting: Place sections on a glass slide and cover with albumin or glycerin.
- Staining: Use organic solvents to remove paraffin, then put the specimen in solutions that contain less alcohol and more water sequentially.
- Common stains include Hematoxylin and eosin or Sudan black
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Description
This quiz explores the fundamental concepts of cell biology, including the structure and function of cells. It delves into various methods for studying cells, such as tissue culture and fixed tissue examination, along with techniques like fixation and dehydration of tissue samples. Test your understanding of the vital role cells play in biological research.
