18 Questions
Aseptic techniques are designed to provide a barrier between the microorganisms in the environment and the ______ cell culture.
sterile
Successful cell culture depends on keeping the cells free from ______.
contamination
For routine cleaning, the work surface should be wiped with ______% ethanol.
70
Wearing personal protective equipment reduces the probability of contamination from ______ as well as dirt and dust from your clothes.
shed skin
The ______ work area should be disinfected thoroughly.
sterile
______ is a key element of aseptic technique.
Sterile handling
Commercial reagents and media undergo strict quality control to ensure their ______
sterility
Always sterilize any reagents, media, or solutions prepared in the laboratory using the appropriate ______ procedure
sterilization
______ is a method of sterilization mentioned in the text
Autoclaving
Sterile handling is important to prevent ______
contamination
Sterile ______ is another method of ensuring sterility
filtration
Tips for aseptic technique in cell culture include sterile ______
filtration
Bacterial contamination is characterized by cells that are a few ______ in diameter.
um
Bacterial contamination can take on a variety of ______ ranging from spheres to rods and spirals.
shapes
Molds are ______ that grow as multicellular filaments called hyphae.
Fungi
Mycoplasma are simple ______ that lack a cell wall.
bacteria
Yeasts are ______ Fungi ranging in size from a few um up to 40 um.
unicellular
Infected cell cultures with bacterial contamination usually appear ______/cloudy.
turbid
Test your knowledge on the requirements of a tissue culture laboratory and aseptic techniques. Learn about maintaining a sterile environment to prevent contamination in cell culture. This quiz is based on Dr. Ahmed Soffar's lecture 4 content.
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