Cell Differentiation and Tissue Culture (MB 303) Lecture 4 Quiz

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18 Questions

Aseptic techniques are designed to provide a barrier between the microorganisms in the environment and the ______ cell culture.

sterile

Successful cell culture depends on keeping the cells free from ______.

contamination

For routine cleaning, the work surface should be wiped with ______% ethanol.

70

Wearing personal protective equipment reduces the probability of contamination from ______ as well as dirt and dust from your clothes.

shed skin

The ______ work area should be disinfected thoroughly.

sterile

______ is a key element of aseptic technique.

Sterile handling

Commercial reagents and media undergo strict quality control to ensure their ______

sterility

Always sterilize any reagents, media, or solutions prepared in the laboratory using the appropriate ______ procedure

sterilization

______ is a method of sterilization mentioned in the text

Autoclaving

Sterile handling is important to prevent ______

contamination

Sterile ______ is another method of ensuring sterility

filtration

Tips for aseptic technique in cell culture include sterile ______

filtration

Bacterial contamination is characterized by cells that are a few ______ in diameter.

um

Bacterial contamination can take on a variety of ______ ranging from spheres to rods and spirals.

shapes

Molds are ______ that grow as multicellular filaments called hyphae.

Fungi

Mycoplasma are simple ______ that lack a cell wall.

bacteria

Yeasts are ______ Fungi ranging in size from a few um up to 40 um.

unicellular

Infected cell cultures with bacterial contamination usually appear ______/cloudy.

turbid

Test your knowledge on the requirements of a tissue culture laboratory and aseptic techniques. Learn about maintaining a sterile environment to prevent contamination in cell culture. This quiz is based on Dr. Ahmed Soffar's lecture 4 content.

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