Podcast
Questions and Answers
What is vital staining?
What is vital staining?
- Staining of a dead cell
- Staining using only chemical reactions
- Staining of a living cell or organoid (correct)
- Staining only outside the body
What type of vital staining involves staining cells inside the living body?
What type of vital staining involves staining cells inside the living body?
- Metachromatic staining
- Intravital staining (correct)
- Histochemical staining
- Supravital staining
Supravital staining is performed on living cells inside the body.
Supravital staining is performed on living cells inside the body.
False (B)
What are the main components used to maintain living tissues and cells in culture?
What are the main components used to maintain living tissues and cells in culture?
Which of the following is a purpose of tissue culture?
Which of the following is a purpose of tissue culture?
What is histochemical staining used for?
What is histochemical staining used for?
What is an example of a specific histochemical stain?
What is an example of a specific histochemical stain?
Metachromatic staining causes stained materials to take on the same color as the applied dye.
Metachromatic staining causes stained materials to take on the same color as the applied dye.
What color do mast cells stain using toluidine blue in metachromatic staining?
What color do mast cells stain using toluidine blue in metachromatic staining?
Flashcards
Vital Staining
Vital Staining
Staining living cells or tissues (organoids).
Intravital Staining
Intravital Staining
Staining live cells inside a living organism.
Supravital Staining
Supravital Staining
Staining live cells outside the living organism.
Tissue Culture
Tissue Culture
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Histochemical Staining
Histochemical Staining
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PAS Staining
PAS Staining
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Orthochromatic Staining
Orthochromatic Staining
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Metachromatic Staining
Metachromatic Staining
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Tissue Culture Applications
Tissue Culture Applications
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Metachromasia
Metachromasia
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Study Notes
Vital Staining
- Vital staining depends on the vitality of the cell or organoid
- Stained cells are stainable when they are living
- This technique is divided into two parts:
- Intravital staining: means staining of living cells inside the living body.
- Example: staining of macrophages by Indian ink
- Supra vital staining: means staining of living cells or organoids outside the body.
- Example: staining of mitochondria by Janus green B and reticulocytes with brilliant crezyl blue.
- Intravital staining: means staining of living cells inside the living body.
Tissue Culture
- Living cells and tissues can be maintained and studied outside the body in culture (in vitro)
- Cells and tissues are grown in complex solutions containing salts, amino acids, and vitamins.
Importance of Tissue Culture
- To study chromosomes of cells in various diseases
- For research purposes such as studying the effect of certain drugs on the cells.
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Description
This quiz explores the principles of vital staining and tissue culture techniques, highlighting the differences between intravital and supravital staining. It also covers the importance of tissue culture in studying cells and their response to various conditions. Enhance your understanding of these essential methods used in biological research.
