Vital Staining and Tissue Culture Techniques
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Questions and Answers

What is vital staining?

  • Staining of a dead cell
  • Staining using only chemical reactions
  • Staining of a living cell or organoid (correct)
  • Staining only outside the body
  • What type of vital staining involves staining cells inside the living body?

  • Metachromatic staining
  • Intravital staining (correct)
  • Histochemical staining
  • Supravital staining
  • Supravital staining is performed on living cells inside the body.

    False

    What are the main components used to maintain living tissues and cells in culture?

    <p>Salts, amino acids, and vitamins</p> Signup and view all the answers

    Which of the following is a purpose of tissue culture?

    <p>To study chromosomes of cells</p> Signup and view all the answers

    What is histochemical staining used for?

    <p>To identify chemical substances in tissues and cells</p> Signup and view all the answers

    What is an example of a specific histochemical stain?

    <p>Toluidine blue</p> Signup and view all the answers

    Metachromatic staining causes stained materials to take on the same color as the applied dye.

    <p>False</p> Signup and view all the answers

    What color do mast cells stain using toluidine blue in metachromatic staining?

    <p>Purple-red</p> Signup and view all the answers

    Study Notes

    Vital Staining

    • Vital staining depends on the vitality of the cell or organoid
    • Stained cells are stainable when they are living
    • This technique is divided into two parts:
      • Intravital staining: means staining of living cells inside the living body.
        • Example: staining of macrophages by Indian ink
      • Supra vital staining: means staining of living cells or organoids outside the body.
        • Example: staining of mitochondria by Janus green B and reticulocytes with brilliant crezyl blue.

    Tissue Culture

    • Living cells and tissues can be maintained and studied outside the body in culture (in vitro)
    • Cells and tissues are grown in complex solutions containing salts, amino acids, and vitamins.

    Importance of Tissue Culture

    • To study chromosomes of cells in various diseases
    • For research purposes such as studying the effect of certain drugs on the cells.

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    Description

    This quiz explores the principles of vital staining and tissue culture techniques, highlighting the differences between intravital and supravital staining. It also covers the importance of tissue culture in studying cells and their response to various conditions. Enhance your understanding of these essential methods used in biological research.

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