Podcast
Questions and Answers
What is a major current problem in tissue culture related to cell cloning?
What is a major current problem in tissue culture related to cell cloning?
- Preservation of particular properties
- Isolation of a specific cell type
- Relatively easy for continuous cell lines
- Poor cloning efficiencies in normal cells (correct)
What is the term for a cell line with the presence of several cell lineages of either similar or distinct phenotypes?
What is the term for a cell line with the presence of several cell lineages of either similar or distinct phenotypes?
- Secondary culture
- Cell strain (correct)
- Tertiary culture
- Primary culture
What gives rise to a tertiary culture in cell cloning?
What gives rise to a tertiary culture in cell cloning?
- Monolayer and suspension cell cloning
- Secondary culture (correct)
- Cloning or physical separation
- Primary culture
What is the term for the process of isolating cloned lines expressing the correct properties?
What is the term for the process of isolating cloned lines expressing the correct properties?
What is the focus of the lecture outline on cell cloning?
What is the focus of the lecture outline on cell cloning?
What is the purpose of cell characterization in cell culture?
What is the purpose of cell characterization in cell culture?
Why is genotype-based authentication preferred over phenotype-based methods?
Why is genotype-based authentication preferred over phenotype-based methods?
What defines a misidentified cell line?
What defines a misidentified cell line?
What is the purpose of authentication in cell culture?
What is the purpose of authentication in cell culture?
What is the identity validation needed for continuous cell lines after prolonged culture or transformation?
What is the identity validation needed for continuous cell lines after prolonged culture or transformation?
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