Cell Cloning and Tissue Culture Terminology
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Questions and Answers

What is a major current problem in tissue culture related to cell cloning?

  • Preservation of particular properties
  • Isolation of a specific cell type
  • Relatively easy for continuous cell lines
  • Poor cloning efficiencies in normal cells (correct)

    • What is the term for a cell line with the presence of several cell lineages of either similar or distinct phenotypes?

  • Secondary culture
  • Cell strain (correct)
  • Tertiary culture
  • Primary culture

    • What gives rise to a tertiary culture in cell cloning?

  • Monolayer and suspension cell cloning
  • Secondary culture (correct)
  • Cloning or physical separation
  • Primary culture

    • What is the term for the process of isolating cloned lines expressing the correct properties?

    <p>Cell cloning</p> Signup and view all the answers

    What is the focus of the lecture outline on cell cloning?

    <p>Important considerations in cell cloning</p> Signup and view all the answers

    What is the purpose of cell characterization in cell culture?

    <p>To validate suspected cross-contamination of cultured cells</p> Signup and view all the answers

    Why is genotype-based authentication preferred over phenotype-based methods?

    <p>Because phenotype-based methods may be misleading</p> Signup and view all the answers

    What defines a misidentified cell line?

    <p>A cell line that no longer corresponds to the donor or species from which it was established</p> Signup and view all the answers

    What is the purpose of authentication in cell culture?

    <p>To prevent widespread use of misidentified cell lines</p> Signup and view all the answers

    What is the identity validation needed for continuous cell lines after prolonged culture or transformation?

    <p>To validate the identity of continuous cell lines after prolonged culture or transformation</p> Signup and view all the answers

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