10 Questions
What is a major current problem in tissue culture related to cell cloning?
Poor cloning efficiencies in normal cells
What is the term for a cell line with the presence of several cell lineages of either similar or distinct phenotypes?
Cell strain
What gives rise to a tertiary culture in cell cloning?
Secondary culture
What is the term for the process of isolating cloned lines expressing the correct properties?
Cell cloning
What is the focus of the lecture outline on cell cloning?
Important considerations in cell cloning
What is the purpose of cell characterization in cell culture?
To validate suspected cross-contamination of cultured cells
Why is genotype-based authentication preferred over phenotype-based methods?
Because phenotype-based methods may be misleading
What defines a misidentified cell line?
A cell line that no longer corresponds to the donor or species from which it was established
What is the purpose of authentication in cell culture?
To prevent widespread use of misidentified cell lines
What is the identity validation needed for continuous cell lines after prolonged culture or transformation?
To validate the identity of continuous cell lines after prolonged culture or transformation
Test your knowledge of cell culture terminology, including primary cultures, subculturing, cell lineages, and cell cloning. Learn about the selection and propagation of specific cell lineages with distinct properties.
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