Podcast
Questions and Answers
Which of the following is a critical step in recombinant DNA technology where a vector is used to deliver a desired gene into a host cell?
Which of the following is a critical step in recombinant DNA technology where a vector is used to deliver a desired gene into a host cell?
- Annealing
- Ligation
- Transformation (correct)
- Denaturation
In the context of gene cloning, what is the primary role of a vector?
In the context of gene cloning, what is the primary role of a vector?
- To identify and isolate the gene of interest from a mixed DNA sample.
- To degrade the host cell's DNA to prevent replication.
- To carry the gene of interest into the host cell for replication. (correct)
- To directly synthesize the protein encoded by the gene of interest.
Which enzyme is primarily responsible for joining DNA fragments together by forming phosphodiester bonds?
Which enzyme is primarily responsible for joining DNA fragments together by forming phosphodiester bonds?
- Reverse transcriptase
- DNA polymerase
- Restriction endonuclease
- Ligase (correct)
If a protein-coding gene is introduced into a bacterium using recombinant DNA technology, what additional element is essential for the bacterium to produce the corresponding protein?
If a protein-coding gene is introduced into a bacterium using recombinant DNA technology, what additional element is essential for the bacterium to produce the corresponding protein?
What is the role of restriction enzymes in biotechnology?
What is the role of restriction enzymes in biotechnology?
In the process of creating a recombinant plasmid, what is the purpose of using the same restriction enzyme to cut both the gene of interest and the plasmid?
In the process of creating a recombinant plasmid, what is the purpose of using the same restriction enzyme to cut both the gene of interest and the plasmid?
A scientist is trying to amplify a specific DNA sequence using PCR, but the reaction fails. Which of the following is least likely to be the cause of the failure?
A scientist is trying to amplify a specific DNA sequence using PCR, but the reaction fails. Which of the following is least likely to be the cause of the failure?
What is the function of gel electrophoresis in biotechnology?
What is the function of gel electrophoresis in biotechnology?
Which of the following is a method used to introduce genes into plant cells to produce genetically modified crops?
Which of the following is a method used to introduce genes into plant cells to produce genetically modified crops?
How does insertional inactivation contribute to identifying recombinant bacteria in plasmid cloning?
How does insertional inactivation contribute to identifying recombinant bacteria in plasmid cloning?
Flashcards
Biotechnology
Biotechnology
The application of organisms, cells, or biological systems to manufacture products or run processes.
Antibiotic
Antibiotic
A chemical substance from a virus or microorganism that prevents the growth of other microorganisms
Genetic Engineering
Genetic Engineering
The technique of using genetically modified organisms to achieve improvements in processes or product yields.
Study Notes
Biotechnology Unit Overview
- A comprehensive overview of the Biotechnology unit for Class 12, aligned with NCERT (National Council of Educational Research and Training) curriculum.
- Includes line-by-line explanation.
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