Biochemistry Practical Manual Overview

<p>Showing completed work to the demonstrator (A)</p> Signup and view all the answers

<p>Using an indelible marker (D)</p> Signup and view all the answers

<p>Pasteur pipettes or autopipettes (C)</p> Signup and view all the answers

<p>Directly into the practical manual (C)</p> Signup and view all the answers

<p>Suitable sized clean dry labelled containers (D)</p> Signup and view all the answers

<p>To serve as energy sources during cellular respiration (C)</p> Signup and view all the answers

<p>Two sugar units bonded together by a glycosidic bond (B)</p> Signup and view all the answers

<p>Glucose (A)</p> Signup and view all the answers

<p>Glycosidic bonds (C)</p> Signup and view all the answers

<p>Cholesterol (C)</p> Signup and view all the answers

<p>Fructose (D)</p> Signup and view all the answers

<p>Starch (C)</p> Signup and view all the answers

<p>Store excess glucose for energy (D)</p> Signup and view all the answers

<p>To store glucose for later use as energy (C)</p> Signup and view all the answers

<p>By hydrolyzing it with amylase (B)</p> Signup and view all the answers

<p>Starch is stored in plants, while glycogen is stored in animals (B)</p> Signup and view all the answers

<p>Cellulose (B)</p> Signup and view all the answers

<p>They assist in digesting cellulose (A)</p> Signup and view all the answers

<p>Sucrose (B)</p> Signup and view all the answers

<p>Cellulose is a structural component of plant cell walls (A)</p> Signup and view all the answers

<p>It is a branched chain of glucose units stored in the liver and muscles (A)</p> Signup and view all the answers

<p>Proteins, lipids, carbohydrates, nucleic acids (D)</p> Signup and view all the answers

<p>Metre, gram, litre (A)</p> Signup and view all the answers

<p>To reduce the number of zeros shown in numerical quantities (A)</p> Signup and view all the answers

<p>The French National Assembly commissioned its design to avoid confusion in measurements (B)</p> Signup and view all the answers

<p>One billionth of a unit (B)</p> Signup and view all the answers

<p>0.00001 meters = 1 x 10^-5 meters (D)</p> Signup and view all the answers

<p>Standardization to ensure universal comprehension (C)</p> Signup and view all the answers

<p>Proficiency in converting between different units (D)</p> Signup and view all the answers

<p>1 x 10^2 (A)</p> Signup and view all the answers

<p>The decimal is moved to the left (A)</p> Signup and view all the answers

<p>8.2057 x 10^4 (D)</p> Signup and view all the answers

<p>They are added (A)</p> Signup and view all the answers

<p>6.0 x 10^-5 (B)</p> Signup and view all the answers

<p>10^2 (B)</p> Signup and view all the answers

<p>9.34 x 10^-4 (A)</p> Signup and view all the answers

<p>6.5 x 10^-3 (B)</p> Signup and view all the answers

<p>Seek assistance to clean the barrel before further use. (D)</p> Signup and view all the answers

<p>The correct type of tip must be used for the pipette model. (B)</p> Signup and view all the answers

<p>Vertically. (D)</p> Signup and view all the answers

<p>You will draw up additional liquid inadvertently. (D)</p> Signup and view all the answers

<p>Eject it into a designated yellow sharps container. (B)</p> Signup and view all the answers

<p>Calibrate the pipette to ensure accuracy and precision. (B)</p> Signup and view all the answers

<p>You risk introducing air bubbles into the liquid. (B)</p> Signup and view all the answers

<p>At a slight angle against the wall of a vessel. (D)</p> Signup and view all the answers

Attending pre-labs and lab sessions is required.

Wear a buttoned-up lab coat during all lab sessions.

Prohibited due to potential hazards of chemicals

Use designated waste bins instead of sinks.

Collect only the needed amount; consider others and use labelled containers.

Labelling is essential to avoid confusion, especially with colorless solutions

Using pipettes, auto pipettes, instead

Show completed workbook to demonstrator

A decimal-based system of measurement adopted globally to replace inconsistent units, simplifying scientific communication.

Symbols preceding a basic unit (like meter, gram) to indicate decimal multiples or fractions of the unit. (e.g., milli-, kilo-)

Fundamental units of measurement in the metric system (e.g., meter, gram, liter), representing basic quantities.

The meter, gram, and liter are the three most common base units in the metric system.

To eliminate confusion caused by various units of measurement used globally and establish a standardized system for science and commerce.

It provides a consistent and easy-to-use system to measure and compare quantities, making scientific data more accessible.

SI prefixes reduce the number of zeros in large or small quantities, making them easier to write and read.

To standardize SI prefixes and ensure a unified system of measurement worldwide.

A way to represent very large or very small numbers concisely using powers of 10.

The number that multiplies the power of 10 in scientific notation.

Indicates how many places to move the decimal point of the coefficient to get the original number.

When multiplying powers of 10, add the exponents.

When dividing powers of 10, subtract the exponents.

Uses a negative exponent on the 10 to indicate a number less than 1.

Uses a positive exponent on the 10 to indicate a number greater than 1.

Always a single digit followed by decimals (if necessary).

The proper type of pipette tip (blue, yellow) must be used. This depends on the desired volume being dispensed, and incorrect tip selection may lead to leakage or inaccuracies.

Ensure the pipette tip is securely attached to the pipette to prevent liquid leakage. A loose or tilted tip can cause problems.

Always use a new, disposable tip with each distinct liquid to prevent contamination. Never use a pipette without its disposable tip.

Never leave a pipette on its side with the tip containing liquid. This can lead to leaking and contamination.

When using an autopipette, press the plunger down slowly until you feel the first resistance. This ensures accurate liquid volumes.

When filling the pipette, ensure the tip is fully submerged in liquid. Avoid drawing air bubbles, as they can lead to inaccurate volumes.

Draw the liquid up into the pipette slowly and evenly. Avoid drawing too quickly, as this can lead to liquid entering the pipette barrel.

To dispense the liquid, place the tip slightly against the vessel wall, press the plunger slowly, and allow any residual liquid to drain. Finally, press the plunger to expel the residual volume.

Simple sugars, like glucose and fructose, that are used as energy sources by cells. They also help build cell structures and other molecules.

Two sugar units, like glucose and fructose, linked together by a glycosidic bond. Sucrose (table sugar) is a common example.

Long chains of monosaccharides bonded together, like starch in plants and glycogen in animals. They store energy and have structural roles.

The chemical bond that connects monosaccharide units to form disaccharides and polysaccharides.

A primary energy source for cells. It is the most common sugar in the human body and stored as glycogen.

The main sugar found in fruits. It has the same chemical formula as glucose (C6H12O6) but a different structure.

A polysaccharide made from long chains of glucose. Plants store excess glucose as starch.

A polysaccharide made from long chains of glucose, used by animals and humans to store excess glucose.

A structural polysaccharide found in plant cell walls, providing support to leaves, stems, and other plant parts. Humans cannot digest cellulose as it passes through the digestive system undigested.

A structural polysaccharide that provides strength and support to the exoskeletons of arthropods (like insects and crustaceans) and the cell walls of fungi.

A sugar that has an aldehyde or keto group in its structure, allowing it to act as a reducing agent in chemical reactions (like Benedict's test).

A chemical test used to detect the presence of reducing sugars in a solution. It involves reacting the sugar with Benedict's reagent, resulting in a color change.

A disaccharide where the anomeric carbons of both sugar units are linked together, preventing the formation of an open-chain aldehyde group necessary for reducing activity.

Glucose, fructose, glyceraldehyde, and galactose are examples of reducing monosaccharides. Many disaccharides, like lactose and maltose, also have a reducing form.