Practical Biochemistry Lab 3: Denaturing of Proteins

Denaturation is a process in which a protein loses its native shape due to the disruption of weak chemical bonds and interactions, becoming biologically inactive.

Heat and violent shaking or agitation

Changing pH denatures proteins because it changes the charges on many of the side chains.

<p>Organic solvents denature proteins by disrupting hydrophobic interactions.</p> Signup and view all the answers

<p>Increasing temperature denatures proteins because it disrupts the attractive forces within the protein structure.</p> Signup and view all the answers

<p>A denatured enzyme ceases to perform its functions.</p> Signup and view all the answers

<p>Heat denaturation or thermal denaturation</p> Signup and view all the answers

<p>Hydrogen bonds, disulfide bonds, and hydrophobic interaction</p> Signup and view all the answers

<p>Cold denaturation</p> Signup and view all the answers

<p>Peptide bonds</p> Signup and view all the answers

<p>Immediate changes in color and consistency</p> Signup and view all the answers

<p>Wait for 15-30 minutes and inspect the state of the egg whites</p> Signup and view all the answers