Bioenergetics II
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Questions and Answers

What is the main purpose of the electron transport chain?

  • To produce NADH and FADH
  • To convert pyruvate to acetyl-CoA
  • To break down glucose into pyruvate
  • To phosphorylate ADP to ATP (correct)
  • Which of the following molecules is an entry point for all major nutrients into the TCA cycle?

  • Acetyl-CoA (correct)
  • Citrate
  • Pyruvate
  • Oxaloacetate
  • What is the role of Monocarboxylate Transport Proteins (MCT) in the process of pyruvate entry into mitochondria?

  • They regulate the activity of the electron transport chain
  • They catalyze the conversion of pyruvate to acetyl-CoA
  • They transport pyruvate across the mitochondrial membrane (correct)
  • They are involved in the synthesis of ATP
  • What is the effect of phosphorylation on the PDH enzyme?

    <p>It inhibits the enzyme (A)</p> Signup and view all the answers

    Which of the following conditions would activate the PDH enzyme?

    <p>Low ATP/ADP ratio (D)</p> Signup and view all the answers

    Which of the following is NOT a product of the TCA cycle?

    <p>Glucose (D)</p> Signup and view all the answers

    How does the reduction potential of the electron transport chain influence the movement of electrons?

    <p>Electrons move from complexes with lower reduction potential to complexes with higher reduction potential (D)</p> Signup and view all the answers

    How many protons are thought to be required for the synthesis of one ATP molecule by ATP synthase?

    <p>Four (A)</p> Signup and view all the answers

    What is the role of the ATP synthase protein complex in the electron transport chain?

    <p>To phosphorylate ADP to ATP (D)</p> Signup and view all the answers

    Which of the following statements accurately describes the relationship between ATP levels and the TCA cycle?

    <p>High ATP levels inhibit the TCA cycle (A)</p> Signup and view all the answers

    Which of the following statements is TRUE regarding the shift from CHO to fat metabolism during prolonged exercise?

    <p>As exercise duration increases, the body preferentially utilizes fat as a fuel source, leading to a decrease in the RER value. (B)</p> Signup and view all the answers

    Which of the following statements accurately describes the role of protein metabolism during exercise?

    <p>Protein metabolism contributes minimally to energy production during typical exercise under adequate nutrient storage. (C)</p> Signup and view all the answers

    Which of the following factors is LEAST LIKELY to influence the availability of CHO as substrate for ATP production during exercise?

    <p>The individual's dietary protein intake. (C)</p> Signup and view all the answers

    What is the primary function of the process known as beta-oxidation in the context of fat catabolism?

    <p>To break down fatty acids into acetyl-CoA, NADH, and FADH, which can then enter the citric acid cycle. (A)</p> Signup and view all the answers

    Which of the following scenarios is most likely to result in an RER value closest to 0.70?

    <p>A 2-hour, low-intensity bike ride in a fasted state. (A)</p> Signup and view all the answers

    During prolonged exercise, what role does blood lactate play in fueling the body?

    <p>Blood lactate is converted into glucose through gluconeogenesis in the liver, providing an additional source of CHO for energy. (A)</p> Signup and view all the answers

    Which of the following accurately describes the relationship between slow glycolysis and ATP production?

    <p>Slow glycolysis is an aerobic process that utilizes oxygen to produce ATP through oxidative phosphorylation in the mitochondria. (B)</p> Signup and view all the answers

    Which of the following factors directly influences the metabolic response to exercise?

    <p>The type of training the individual has undertaken. (A)</p> Signup and view all the answers

    Based on the provided information, what is the approximate percentage of fat and CHO utilization when the RER is 0.85?

    <p>50% Fat, 50% CHO (C)</p> Signup and view all the answers

    What is the main purpose of deamination in protein catabolism?

    <p>To remove the amino group from amino acids, allowing their carbon skeletons to be used for energy production. (B)</p> Signup and view all the answers

    What is the primary role of the Krebs cycle in aerobic ATP production?

    <p>To oxidize substrates and produce NADH and FADH, which carry electrons to the electron transport chain. (B)</p> Signup and view all the answers

    Which of the following energy systems provides ATP for short-duration, high-intensity exercise?

    <p>ATP-PCr system (A)</p> Signup and view all the answers

    In the Electron Transport Chain (ETC), what is the ultimate fate of electrons extracted from NADH and FADH2?

    <p>They are transferred to oxygen to form water. (A)</p> Signup and view all the answers

    During fat catabolism, how many ATP molecules are generated from the breakdown of a single triacylglycerol molecule, considering both fatty acid and glycerol components?

    <p>460 ATP (C)</p> Signup and view all the answers

    What is the primary function of the outer membrane of the mitochondria?

    <p>To act as a barrier, maintaining essential internal components and regulating the entry of molecules. (D)</p> Signup and view all the answers

    Which of the following statements accurately describes the efficiency of energy conservation during fatty acid oxidation?

    <p>The efficiency of energy conservation during fatty acid oxidation is slightly higher compared to glucose oxidation. (C)</p> Signup and view all the answers

    Which of the following accurately describes the difference between anaerobic and aerobic metabolism?

    <p>All of the above. (D)</p> Signup and view all the answers

    Which of the following statements accurately describes the role of ketone bodies in energy metabolism?

    <p>Ketone bodies are formed primarily during prolonged fasting or strenuous exercise when glucose availability is low, serving as an alternative fuel source for the brain. (C)</p> Signup and view all the answers

    What is the primary factor that influences whether carbohydrates or fats are used as fuel during exercise?

    <p>Intensity of the exercise (A)</p> Signup and view all the answers

    What role does acetyl-CoA play in skeletal muscle metabolism during exercise?

    <p>It regulates the entry point for fatty acids and glucose. (D)</p> Signup and view all the answers

    Why does glycogen become a major contributor to energy supply during high intensity exercise?

    <p>It is more readily available than fatty acids. (B)</p> Signup and view all the answers

    What physiological change primarily activates lipolysis during exercise?

    <p>Higher levels of epinephrine (A)</p> Signup and view all the answers

    What occurs if acetyl-CoA levels decline during sustained submaximal exercise?

    <p>PDH activity is increased to enhance energy supply. (C)</p> Signup and view all the answers

    What is the primary energy system utilized during short bouts of intense exercise?

    <p>ATP-PCr system (B)</p> Signup and view all the answers

    During maximal intensity exercise, what happens to fatty acid oxidation rates as the intensity increases?

    <p>They decline significantly past 85% of VO2 max. (D)</p> Signup and view all the answers

    What is the significance of the 'crossover' concept in exercise physiology?

    <p>It describes the transition from fat to carbohydrate as primary fuel source. (C)</p> Signup and view all the answers

    What describes the oxygen deficit in the context of initiating exercise?

    <p>A period of anaerobic ATP contribution (C)</p> Signup and view all the answers

    What happens to the energy substrate proportion during submaximal exercise as one approaches 50-80% of VO2 max?

    <p>Both carbohydrates and fats are used equally. (D)</p> Signup and view all the answers

    Which of the following statements accurately describes the role of carnitine in lipid metabolism?

    <p>Carnitine is a carrier molecule that facilitates the transport of fatty acids across the mitochondrial membrane. (D)</p> Signup and view all the answers

    What is the primary source of energy for muscle during exercise at moderate intensity (65-80% VO2 max)?

    <p>Intramuscular triglycerides (IMTG) (A)</p> Signup and view all the answers

    Which of the following molecules is NOT a direct regulator of ATP production rate?

    <p>Cytochrome-C oxidase (D)</p> Signup and view all the answers

    What is the primary function of lipase in lipid metabolism?

    <p>To break down triglycerides into fatty acids and glycerol. (C)</p> Signup and view all the answers

    How many ATP molecules are produced per molecule of glucose during glycolysis?

    <p>6 (A)</p> Signup and view all the answers

    What is the main reason why lipid metabolism is slower than carbohydrate metabolism?

    <p>The breakdown of lipids requires more complex enzymatic reactions. (A)</p> Signup and view all the answers

    What is the role of fatty acid translocase (FAT/CD36) in lipid metabolism?

    <p>It facilitates the uptake of fatty acids into muscle cells. (C)</p> Signup and view all the answers

    How does an increase in insulin levels affect lipid metabolism?

    <p>It inhibits lipase activity. (D)</p> Signup and view all the answers

    What is the primary factor that limits the rate of lipid metabolism?

    <p>The capacity of the mitochondria to oxidize fatty acids. (D)</p> Signup and view all the answers

    Why is the energy yield of glycogen slightly higher than that of glucose?

    <p>Glycogen molecules contain a small number of extra glucose units that contribute to the higher energy yield. (C)</p> Signup and view all the answers

    Flashcards

    Substrate Level Phosphorylation

    A process that generates ATP using energy from metabolic reactions without oxygen, like anaerobic metabolism and glycolysis.

    Oxidative Level Phosphorylation

    A method of ATP production that occurs through aerobic metabolism involving glucose oxidation, beta oxidation, and more.

    Krebs Cycle

    A crucial part of aerobic metabolism that oxidizes substrates to produce NADH and FADH for the Electron Transport Chain.

    Electron Transport Chain (ETC)

    A series of proteins in the mitochondrial inner membrane that oxidize NADH and FADH to produce ATP through oxidative phosphorylation.

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    Mitochondria Respiration

    The process occurring in mitochondria where pyruvate is converted to ATP and CO2, especially during rest or steady activity.

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    Respiratory Exchange Ratio (RER)

    The ratio of carbon dioxide produced to oxygen consumed during exercise (VCO2/VO2).

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    RER Values and Fuel Utilization

    RER indicates percentage of fat and carbohydrate utilization: 0.70 = 100% fat, 1.00 = 100% CHO.

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    Fat Oxidation Reaction

    Palmitic acid (C16H32O2) oxidation produces 16CO2 and ATP with RER = 0.70.

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    Glycogen Depletion

    Exhaustion of blood glucose and muscle glycogen after 90-120 minutes of intense exercise.

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    Protein in Exercise

    Protein contributes roughly 2% to energy production, possibly rising to 5-15% in prolonged exercise.

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    Mobilization

    Breakdown of adipose and intramuscular triglyceride to release fatty acids.

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    FFA Circulation

    Transport of Free Fatty Acids from adipose tissue to muscles.

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    FFA Uptake

    The entry of Free Fatty Acids into muscle cells from the bloodstream.

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    Beta-Oxidation

    Process of breaking down fatty acids to produce acetyl-CoA, NADH, and FADH.

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    Total ATP from Fat

    One triacylglycerol yields 460 ATP, higher energy yield than glucose.

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    Gluconeogenesis

    Process of converting protein to glucose, mainly in the liver during low energy availability.

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    Protein Catabolism

    Breakdown of protein into amino acids, used for energy when needed.

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    Energy Systems

    Different metabolic pathways (ATP-PCr, carbs, fats) that generate ATP under varying exercise conditions.

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    ETC Controllers

    ATP, ADP, NADH, FADH, and oxygen regulate the electron transport chain.

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    Glycolysis Energy Yield

    Glycolysis produces 2 ATP and 2 NADH, totaling 6 ATP in mitochondria.

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    Total ATP from Glucose

    Complete oxidation of one glucose molecule yields 38 ATP.

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    Stages of Carbohydrate Oxidation

    Three stages: Glycolysis, Krebs Cycle, Electron Transport Chain.

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    Lipolysis

    Lipolysis is the breakdown of triglycerides into fatty acids and glycerol.

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    Lipase Function

    Lipase is the enzyme that regulates lipolysis, sensitive to hormones.

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    Free Fatty Acid Transport

    FFA is transported to muscles via albumin and specific transport proteins.

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    Beta-Oxidation Process

    Beta-oxidation breaks down fatty acyl-CoA generating acetyl-CoA, NADH, and FADH.

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    Carnitine Shuttle

    The carnitine shuttle allows FFA to cross the mitochondrial membrane for oxidation.

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    Intramuscular Triglycerides

    IMTG are lipid droplets in muscles that serve as energy sources during exercise.

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    Pyruvate Dehydrogenase (PDH)

    Enzyme that converts pyruvate into Acetyl-CoA, crucial for energy production.

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    Acetyl-CoA

    Key metabolite that enters the TCA Cycle, derived from pyruvate and other macronutrients.

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    TCA Cycle

    Series of 9 reactions that convert Acetyl-CoA, releasing CO2 and producing energy carriers NADH and FADH.

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    ATP Synthase

    Enzyme that synthesizes ATP from ADP using a proton gradient across the mitochondrial membrane.

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    NADH

    Reducing agent generated in glycolysis and TCA, donates electrons to the ETC.

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    Phosphorylation Control

    Process regulating PDH activity; inhibited by phosphorylation and activated by dephosphorylation.

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    Oxidative Phosphorylation

    Process by which ATP is produced in the ETC using energy from electrons and protons from NADH and FADH.

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    Reduction Potential

    Measure of an electron's affinity; the basis for electron movement through the ETC.

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    TCA Cycle Products

    Results from TCA include 1 ATP, 4 NADH, 1 FADH, and 2 CO2 per cycle.

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    Energy Metabolism Increase

    Exercise can increase whole body energy metabolism by up to 20 times basal levels.

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    Glycogen Stores

    Fatigue is linked to glycogen stores and hypoglycemia.

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    Acetyl-CoA Role

    Acetyl-CoA is a key regulator for fatty acid and glucose entry into metabolism.

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    Absolute vs Relative Intensity

    Absolute intensity is total fuel quantity, while relative is the proportion of CHO to FAT used.

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    ATP-PCr System

    Immediate energy for short, intense exercise comes from the ATP-PCr system, lasting 5-15 seconds.

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    Nonaerobic Contribution

    During brief, high-intensity efforts, nonaerobic sources provide about 90% of energy.

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    Submaximal Exercise Goals

    During submaximal exercise, the goal is to establish steady-state ATP supply through aerobic means.

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    Crossover Concept

    The crossover concept illustrates the shift from fat to carbohydrate metabolism as exercise intensity increases.

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    Oxygen Deficit

    Oxygen uptake lags at the start of exercise, indicating anaerobic contributions initially.

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    Lipid Delivery at High Intensity

    At higher intensities (85% VO2 max), fatty acid oxidation decreases while lipolysis remains high but delivery may drop.

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    Study Notes

    Bioenergetics II

    • Bioenergetics II is a course offered by the School of Medicine & Health Sciences, at The George Washington University.
    • The course is taught by Donal Murray, PhD, CSCS.
    • The course code is PT 8202.

    Metabolic Pathways

    • Metabolic pathways occur at two levels: substrate level phosphorylation and oxidative level phosphorylation.
    • Substrate level phosphorylation involves anaerobic metabolism (immediate energy systems) and glycolysis (anaerobic or fast glycolysis).
    • Oxidative level phosphorylation involves aerobic metabolism (aerobic metabolism, oxidation of glucose, beta oxidation, and protein catabolism and gluconeogenesis).
    • The choice of energy system depends on oxygen availability (oxygen available or oxygen unavailable).

    Glycolysis

    • Glycolysis occurs in two ways: Glycolysis from glucose (blood glucose) and Glycolysis from glycogen (muscle glycogen).
    • For glycolysis from Glucose, 2 ATP are used (hexokinase and phosphofructokinase) and 4 ATP are produced (2 ATP per glyceraldehyde-3-phosphate). The net yield is 2 ATP per glucose molecule.
    • For glycolysis from Glycogen, 1 ATP is used (only at phosphofructokinase step) and 4 ATP are produced (same as glucose). The net yield is 3 ATP per glycogen-derived glucose.

    Additional Energy Carriers

    • Both glucose and glycogen breakdown produce 2 NADH per glucose unit.
    • Oxidative phosphorylation yields ~5 ATP (2.5 per NADH) if oxygen is present.

    Slow Glycolysis (Aerobic)

    • Slow glycolysis is utilized in activities lasting over 2 minutes.
    • It occurs in the mitochondria.
    • It involves the Krebs Cycle (TCA, citric acid cycle) and Electron Transport Chain.

    Aerobic ATP Production

    • Krebs cycle (citric acid cycle, TCA) completes the oxidation of substrates that produces NADH and FADH for the electron transport chain.
    • Oxidative phosphorylation, electrons are oxidized from NADH and FADH across a series of carriers and produce ATP.
    • Hydrogen (H+) from NADH and FADH are accepted by oxygen (O2) to form water.

    Mitochondria Respiration

    • During rest and steady state activity, most pyruvate is not converted to lactate; it is taken up into the mitochondria.
    • The Krebs cycle happens in the matrix of the mitochondria.
    • The Respiratory Chain, or Electron Transport Chain (ETC), is located on the mitochondrial inner membrane.

    Mitochondrial Functions

    • The outer mitochondrial membrane is a barrier that controls the movement of materials (like NADH).
    • The outer membrane also contains transport mechanisms for controlling material influx and efflux.
    • The inner mitochondrial membrane (cristae membrane) is the main site for oxidative phosphorylation.
    • The cristae membrane contains protein complexes (F-complexes) responsible for the production of ATP.
    • The mitochondrial matrix contains nearly 50% of the mitochondrial proteins, along with the enzymes responsible for the Krebs Cycle and lactate dehydrogenase.

    Oxidation

    • Biologic burning of macronutrients provides the energy required for phosphorylation.
    • Oxidation occurs on the inner membrane of mitochondria.
    • Electrons are transferred from NADH and FADH2 to molecular oxygen (O2).
    • Oxygen then releases and transfers chemical energy to combine ADP and a phosphate ion from energy to form ATP.
    • 90% of ATP synthesis happens in the respiratory chain via oxidative reactions coupled with phosphorylation.

    Prerequisites of OXPHOS

    • Three prerequisites are needed for oxidative phosphorylation (OXPHOS):
    1. availability of reducing agents NADH or FADH2,
    2. presence of a terminal oxidizing agent (oxygen), and
    3. sufficient quantity of enzymes and metabolic machinery to transfer energy.

    Pyruvate Entry into Mitochondria

    • Both lactate and pyruvate are released into the blood and gain entry into the mitochondrion via Monocarboxylate Transport Proteins (MCT).

    Acetyl Coenzyme A

    • Pyruvate dehydrogenase catalyzes the conversion of pyruvate to Acetyl-CoA, a committed step.
    • Co2, NADH are additional products.
    • This pathway is critical for determining the rate of lactate formation and substrate availability for mitochondrial oxidation.

    PDH Reaction

    • Pyruvate converts to Acetyl-CoA via a reaction that loses a carbon atom as CO2.
    • This generates two pyruvates, two Acetyl-CoA, two CO2, and two NADH.
    • Acetyl-CoA is important as it is the entry point for carbohydrates, fats, and proteins into the TCA cycle.

    TCA Cycle

    • The TCA cycle (or Krebs Cycle) is a metabolic pathway with 9 reactions that produce NADH and FADH2 when acetyl-CoA is decarboxylated.
    • Molecules can enter and leave the cycle at any stage.
    • Oxaloacetate has 4 carbons. It starts the process again after 2 carbons are produced from Acetyl-CoA.

    Summary of TCA Regulators

    • PDH is regulated by ATP/ADP, Acet-CoA/CoA, NADH/NAD.
    • Citrate synthase is regulated by ATP/ADP
    • Dehydrogenase enzymes are regulated by ATP/ADP and NADH/NAD.
    • Under adequate ATP and NADH conditions, enzymes are inhibited leading to reduced TCA function.
    • Conversely, decreased ATP and NADH stimulates TCA function.

    TCA Products

    • 1 ATP
    • 4 NADH
    • 1 FADH2
    • 2 CO2

    Net Energy Transfer from Glucose

    • Glucose yields 38 ATP molecules.
    • Glycolysis generates 6 ATP.
    • Pyruvate-to-Acetyl-CoA generates 6 ATP.
    • TCA produces 24 ATP

    Anaerobic Respiration

    • Glycolysis and fermentation are two processes with different products and rate-limiting enzymes.
    • Glycolysis with lactate dehydrogenase produces lactate from pyruvate. Fermentation with alcohol dehydrogenase produces ethanol from pyruvate.

    Electron Transport Chain (ETC)

    • The ETC is a series of 4 complexes located on the inner mitochondrial membrane.
    • NADH and FADH are oxidized. The end product is water.
    • The main purpose is to phosphorylate ADP to ATP.

    ATP Synthase

    • ATP synthase is a protein complex found on the inner mitochondrial membrane.
    • It plays a role in the phosphorylation of ADP to ATP.
    • The synthesis of one ATP is associated with the flowing of protons through F0/F1 complex.

    ETC Control

    • Pyruvate, ADP, Pi, NADH, FADH, and oxygen have been identified as possible controllers of ATP production rate.
    • Oxygen availability and concentration of cytochrome C oxidase can impact ATP production during exercise.
    • A small decline in ADP levels can stimulate ATP production.

    Energy Yield per Molecule of Glucose

    • Each glucose molecule yields 38 ATP molecules.

    Oxidation of Carbohydrate

    • The oxidation of carbohydrate has three primary stages: Glycolysis, Krebs Cycle, and Electron Transport Chain.

    Beta-Oxidation

    • Beta-oxidation is the process of breaking down fatty acids to produce Acetyl-CoA, NADH, and FADH2, which then go into the TCA cycle.
    • This process happens in the mitochondria.

    Energy Release from Lipids

    • Stored fat (triacylglycerols) are the most plentiful source of potential energy.
    • The breakdown of triacylglycerols yields roughly 460 ATP molecules.
    • Fatty acids need a carrier protein (albumin) to be transported into muscle cells.
    • The carrier protein can be impacted by weight loss.
    • Enzymes like acyl-CoA synthase are involved in the initial steps of the process to produce fattyacyl-CoA.
    • This process occurs in the mitochondria.

    Lipid Metabolism

    • Lipolysis is the breakdown of triglycerides into fatty acids and glycerol.
    • Lipase is the primary enzyme involved.
    • Hormonal factors regulate lipase activity.
    • Lipid metabolism is slower than carbohydrate metabolism.
    • Lipid metabolism can contribute to meeting energy needs during prolonged exercise.

    Lipid Metabolism (Additional Points)

    • Lipolysis occurs in adipose tissue (primarily) and intramuscular triglycerides.
    • Fatty acids are insoluble and need carrier proteins like albumin in the blood to get into the muscle.
    • Acyl-CoA Synthase activates fatty acids by adding Coenzyme A producing fatty acyl-CoA.
    • Two ATP are required for this activation step.

    ATP-PC System

    • The ATP-PC system is a quick source of energy used in short, high-intensity activities and relies on the reaction of Creatine Phosphate (CP) with ADP to produce ATP.
    • Creatine kinase is a rate-limiting enzyme.

    Protein Metabolism

    • Protein metabolism provides minimal energy under normal exercise conditions.
    • Inadequate caloric intake or prolonged exercise can cause protein breakdown.
    • Amino acids are cleaved from the protein strand and undergo deamination to produce an amino group and a carbon skeleton.
    • The carbon skeleton is then incorporated into other metabolic pathways.
    • Protein catabolism is generally not desirable as a source of energy.

    Applications & Considerations of Bioenergetics

    • Bioenergetics considerations become crucial when tailoring exercise interventions and understanding responses in various situations. This includes varying exercise intensity/duration, different training modalities, and the type of substrate used for energy generation.

    Exercise Intensity

    • Exercise intensity, both absolute and relative, influences the proportion of fuels used.
    • Absolute work rate is the total quantity of fuel needed for working muscles.
    • Relative intensity dictates the proportion of carbohydrates and fats used by the working muscles during an exercise session.

    Substrate Metabolism During Exercise

    • Carbohydrates and fats are the primary sources of fuel used for metabolic processes during exercise sessions.

    Short Bouts of Intense Exercise

    • Short bursts of intense exercise rely heavily on the ATP-PCr system.
    • ADP and ATP changes up-regulate PFK and PK reactions leading to increased glycolysis.
    • AMP and ADP upregulate the rate processes.

    Submaximal Exercise Intensity

    • Submaximal exercise leads to a steady state of ATP supply through aerobic means.
    • The system takes time to 'catch up' to ATP demand.
    • Increased NADH/NAD and ATP/ADP ratio influence the rates of TCA enzyme activity.
    • Epinephrine activates lipase and lipolysis to maintain the increase of fatty acid oxidation.

    Submaximal Exercise Intensity (Additional Points)

    • Steady-state oxygen uptake is reached between 1-4 minutes of submaximal exercise.
    • Anaerobic pathways initially provide ATP for the activity; however, ATP is met by aerobic processes once steady-state oxygen uptake is reached.

    Oxygen Uptake During Submaximal Exercise

    • Oxygen uptake rapidly increases during submaximal exercise, reaching a steady state within 1-4 minutes.
    • While there is an oxygen deficit at the start, as the activity continues ATP requires are met by aerobic pathways.
    • Anaerobic sources initially contribute to the immediate ATP need.

    Maximal Exercise Intensity

    • Maximal exercise intensity requires a high rate of ATP turnover and increase NADH/NAD and ATP/ADP ratios.
    • Increasing AMP activates PFK and PK, increasing glycolysis.
    • An increase in pyruvate leads to increasing levels of pyruvate, which ramps up the PDH reaction.
    • Epinephrine leads to increased plasma levels, which releases stored muscle glycogen.

    Lipolysis During Maximal Exercise

    • Fatty acid oxidation rates are reduced at an intensity level exceeding 85% of VO2 max.
    • Blood flow to and from adipose tissue changes.
    • The delivery of FA to contracting muscles may be reduced during higher intensities.

    Estimation of Fuel Utilization During Exercise

    • The respiratory exchange ratio (RER) is used to estimate the intensity level.
    • RER is calculated from VCO2/VO2.
    • The RER can differentiate between the different fuels used during maximal exercise and the different substrate usage.

    Sources of Fuel During Exercise

    • Muscle glycogen, blood glucose, plasma FFA, and intramuscular triglycerides are used as fuel sources for exercise.

    Glycogen Depletion

    • The availability of carbohydrates for energy production is finite.
    • Glycogen stores are used up after approximately 90-120 minutes of exercise and further depletes ATP production.
    • Performance levels are reduced due to glycogen depletion.

    Additional Notes:

    • The provided summaries are accurate factual accounts of concepts covered. Supplementary diagrams and further information may help to clarify each concept.

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    Test your knowledge on the electron transport chain and the TCA cycle with this quiz. Explore key concepts such as the role of various proteins and enzymes involved in cellular respiration. Answer questions about the function of ATP synthase and the metabolic shifts during exercise.

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