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Questions and Answers
What is the main purpose of the electron transport chain?
What is the main purpose of the electron transport chain?
Which of the following molecules is an entry point for all major nutrients into the TCA cycle?
Which of the following molecules is an entry point for all major nutrients into the TCA cycle?
What is the role of Monocarboxylate Transport Proteins (MCT) in the process of pyruvate entry into mitochondria?
What is the role of Monocarboxylate Transport Proteins (MCT) in the process of pyruvate entry into mitochondria?
What is the effect of phosphorylation on the PDH enzyme?
What is the effect of phosphorylation on the PDH enzyme?
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Which of the following conditions would activate the PDH enzyme?
Which of the following conditions would activate the PDH enzyme?
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Which of the following is NOT a product of the TCA cycle?
Which of the following is NOT a product of the TCA cycle?
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How does the reduction potential of the electron transport chain influence the movement of electrons?
How does the reduction potential of the electron transport chain influence the movement of electrons?
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How many protons are thought to be required for the synthesis of one ATP molecule by ATP synthase?
How many protons are thought to be required for the synthesis of one ATP molecule by ATP synthase?
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What is the role of the ATP synthase protein complex in the electron transport chain?
What is the role of the ATP synthase protein complex in the electron transport chain?
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Which of the following statements accurately describes the relationship between ATP levels and the TCA cycle?
Which of the following statements accurately describes the relationship between ATP levels and the TCA cycle?
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Which of the following statements is TRUE regarding the shift from CHO to fat metabolism during prolonged exercise?
Which of the following statements is TRUE regarding the shift from CHO to fat metabolism during prolonged exercise?
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Which of the following statements accurately describes the role of protein metabolism during exercise?
Which of the following statements accurately describes the role of protein metabolism during exercise?
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Which of the following factors is LEAST LIKELY to influence the availability of CHO as substrate for ATP production during exercise?
Which of the following factors is LEAST LIKELY to influence the availability of CHO as substrate for ATP production during exercise?
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What is the primary function of the process known as beta-oxidation in the context of fat catabolism?
What is the primary function of the process known as beta-oxidation in the context of fat catabolism?
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Which of the following scenarios is most likely to result in an RER value closest to 0.70?
Which of the following scenarios is most likely to result in an RER value closest to 0.70?
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During prolonged exercise, what role does blood lactate play in fueling the body?
During prolonged exercise, what role does blood lactate play in fueling the body?
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Which of the following accurately describes the relationship between slow glycolysis and ATP production?
Which of the following accurately describes the relationship between slow glycolysis and ATP production?
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Which of the following factors directly influences the metabolic response to exercise?
Which of the following factors directly influences the metabolic response to exercise?
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Based on the provided information, what is the approximate percentage of fat and CHO utilization when the RER is 0.85?
Based on the provided information, what is the approximate percentage of fat and CHO utilization when the RER is 0.85?
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What is the main purpose of deamination in protein catabolism?
What is the main purpose of deamination in protein catabolism?
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What is the primary role of the Krebs cycle in aerobic ATP production?
What is the primary role of the Krebs cycle in aerobic ATP production?
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Which of the following energy systems provides ATP for short-duration, high-intensity exercise?
Which of the following energy systems provides ATP for short-duration, high-intensity exercise?
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In the Electron Transport Chain (ETC), what is the ultimate fate of electrons extracted from NADH and FADH2?
In the Electron Transport Chain (ETC), what is the ultimate fate of electrons extracted from NADH and FADH2?
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During fat catabolism, how many ATP molecules are generated from the breakdown of a single triacylglycerol molecule, considering both fatty acid and glycerol components?
During fat catabolism, how many ATP molecules are generated from the breakdown of a single triacylglycerol molecule, considering both fatty acid and glycerol components?
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What is the primary function of the outer membrane of the mitochondria?
What is the primary function of the outer membrane of the mitochondria?
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Which of the following statements accurately describes the efficiency of energy conservation during fatty acid oxidation?
Which of the following statements accurately describes the efficiency of energy conservation during fatty acid oxidation?
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Which of the following accurately describes the difference between anaerobic and aerobic metabolism?
Which of the following accurately describes the difference between anaerobic and aerobic metabolism?
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Which of the following statements accurately describes the role of ketone bodies in energy metabolism?
Which of the following statements accurately describes the role of ketone bodies in energy metabolism?
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What is the primary factor that influences whether carbohydrates or fats are used as fuel during exercise?
What is the primary factor that influences whether carbohydrates or fats are used as fuel during exercise?
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What role does acetyl-CoA play in skeletal muscle metabolism during exercise?
What role does acetyl-CoA play in skeletal muscle metabolism during exercise?
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Why does glycogen become a major contributor to energy supply during high intensity exercise?
Why does glycogen become a major contributor to energy supply during high intensity exercise?
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What physiological change primarily activates lipolysis during exercise?
What physiological change primarily activates lipolysis during exercise?
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What occurs if acetyl-CoA levels decline during sustained submaximal exercise?
What occurs if acetyl-CoA levels decline during sustained submaximal exercise?
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What is the primary energy system utilized during short bouts of intense exercise?
What is the primary energy system utilized during short bouts of intense exercise?
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During maximal intensity exercise, what happens to fatty acid oxidation rates as the intensity increases?
During maximal intensity exercise, what happens to fatty acid oxidation rates as the intensity increases?
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What is the significance of the 'crossover' concept in exercise physiology?
What is the significance of the 'crossover' concept in exercise physiology?
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What describes the oxygen deficit in the context of initiating exercise?
What describes the oxygen deficit in the context of initiating exercise?
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What happens to the energy substrate proportion during submaximal exercise as one approaches 50-80% of VO2 max?
What happens to the energy substrate proportion during submaximal exercise as one approaches 50-80% of VO2 max?
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Which of the following statements accurately describes the role of carnitine in lipid metabolism?
Which of the following statements accurately describes the role of carnitine in lipid metabolism?
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What is the primary source of energy for muscle during exercise at moderate intensity (65-80% VO2 max)?
What is the primary source of energy for muscle during exercise at moderate intensity (65-80% VO2 max)?
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Which of the following molecules is NOT a direct regulator of ATP production rate?
Which of the following molecules is NOT a direct regulator of ATP production rate?
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What is the primary function of lipase in lipid metabolism?
What is the primary function of lipase in lipid metabolism?
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How many ATP molecules are produced per molecule of glucose during glycolysis?
How many ATP molecules are produced per molecule of glucose during glycolysis?
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What is the main reason why lipid metabolism is slower than carbohydrate metabolism?
What is the main reason why lipid metabolism is slower than carbohydrate metabolism?
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What is the role of fatty acid translocase (FAT/CD36) in lipid metabolism?
What is the role of fatty acid translocase (FAT/CD36) in lipid metabolism?
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How does an increase in insulin levels affect lipid metabolism?
How does an increase in insulin levels affect lipid metabolism?
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What is the primary factor that limits the rate of lipid metabolism?
What is the primary factor that limits the rate of lipid metabolism?
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Why is the energy yield of glycogen slightly higher than that of glucose?
Why is the energy yield of glycogen slightly higher than that of glucose?
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Flashcards
Substrate Level Phosphorylation
Substrate Level Phosphorylation
A process that generates ATP using energy from metabolic reactions without oxygen, like anaerobic metabolism and glycolysis.
Oxidative Level Phosphorylation
Oxidative Level Phosphorylation
A method of ATP production that occurs through aerobic metabolism involving glucose oxidation, beta oxidation, and more.
Krebs Cycle
Krebs Cycle
A crucial part of aerobic metabolism that oxidizes substrates to produce NADH and FADH for the Electron Transport Chain.
Electron Transport Chain (ETC)
Electron Transport Chain (ETC)
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Mitochondria Respiration
Mitochondria Respiration
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Respiratory Exchange Ratio (RER)
Respiratory Exchange Ratio (RER)
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RER Values and Fuel Utilization
RER Values and Fuel Utilization
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Fat Oxidation Reaction
Fat Oxidation Reaction
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Glycogen Depletion
Glycogen Depletion
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Protein in Exercise
Protein in Exercise
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Mobilization
Mobilization
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FFA Circulation
FFA Circulation
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FFA Uptake
FFA Uptake
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Beta-Oxidation
Beta-Oxidation
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Total ATP from Fat
Total ATP from Fat
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Gluconeogenesis
Gluconeogenesis
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Protein Catabolism
Protein Catabolism
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Energy Systems
Energy Systems
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ETC Controllers
ETC Controllers
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Glycolysis Energy Yield
Glycolysis Energy Yield
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Total ATP from Glucose
Total ATP from Glucose
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Stages of Carbohydrate Oxidation
Stages of Carbohydrate Oxidation
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Lipolysis
Lipolysis
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Lipase Function
Lipase Function
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Free Fatty Acid Transport
Free Fatty Acid Transport
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Beta-Oxidation Process
Beta-Oxidation Process
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Carnitine Shuttle
Carnitine Shuttle
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Intramuscular Triglycerides
Intramuscular Triglycerides
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Pyruvate Dehydrogenase (PDH)
Pyruvate Dehydrogenase (PDH)
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Acetyl-CoA
Acetyl-CoA
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TCA Cycle
TCA Cycle
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ATP Synthase
ATP Synthase
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NADH
NADH
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Phosphorylation Control
Phosphorylation Control
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Oxidative Phosphorylation
Oxidative Phosphorylation
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Reduction Potential
Reduction Potential
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TCA Cycle Products
TCA Cycle Products
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Energy Metabolism Increase
Energy Metabolism Increase
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Glycogen Stores
Glycogen Stores
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Acetyl-CoA Role
Acetyl-CoA Role
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Absolute vs Relative Intensity
Absolute vs Relative Intensity
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ATP-PCr System
ATP-PCr System
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Nonaerobic Contribution
Nonaerobic Contribution
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Submaximal Exercise Goals
Submaximal Exercise Goals
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Crossover Concept
Crossover Concept
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Oxygen Deficit
Oxygen Deficit
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Lipid Delivery at High Intensity
Lipid Delivery at High Intensity
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Study Notes
Bioenergetics II
- Bioenergetics II is a course offered by the School of Medicine & Health Sciences, at The George Washington University.
- The course is taught by Donal Murray, PhD, CSCS.
- The course code is PT 8202.
Metabolic Pathways
- Metabolic pathways occur at two levels: substrate level phosphorylation and oxidative level phosphorylation.
- Substrate level phosphorylation involves anaerobic metabolism (immediate energy systems) and glycolysis (anaerobic or fast glycolysis).
- Oxidative level phosphorylation involves aerobic metabolism (aerobic metabolism, oxidation of glucose, beta oxidation, and protein catabolism and gluconeogenesis).
- The choice of energy system depends on oxygen availability (oxygen available or oxygen unavailable).
Glycolysis
- Glycolysis occurs in two ways: Glycolysis from glucose (blood glucose) and Glycolysis from glycogen (muscle glycogen).
- For glycolysis from Glucose, 2 ATP are used (hexokinase and phosphofructokinase) and 4 ATP are produced (2 ATP per glyceraldehyde-3-phosphate). The net yield is 2 ATP per glucose molecule.
- For glycolysis from Glycogen, 1 ATP is used (only at phosphofructokinase step) and 4 ATP are produced (same as glucose). The net yield is 3 ATP per glycogen-derived glucose.
Additional Energy Carriers
- Both glucose and glycogen breakdown produce 2 NADH per glucose unit.
- Oxidative phosphorylation yields ~5 ATP (2.5 per NADH) if oxygen is present.
Slow Glycolysis (Aerobic)
- Slow glycolysis is utilized in activities lasting over 2 minutes.
- It occurs in the mitochondria.
- It involves the Krebs Cycle (TCA, citric acid cycle) and Electron Transport Chain.
Aerobic ATP Production
- Krebs cycle (citric acid cycle, TCA) completes the oxidation of substrates that produces NADH and FADH for the electron transport chain.
- Oxidative phosphorylation, electrons are oxidized from NADH and FADH across a series of carriers and produce ATP.
- Hydrogen (H+) from NADH and FADH are accepted by oxygen (O2) to form water.
Mitochondria Respiration
- During rest and steady state activity, most pyruvate is not converted to lactate; it is taken up into the mitochondria.
- The Krebs cycle happens in the matrix of the mitochondria.
- The Respiratory Chain, or Electron Transport Chain (ETC), is located on the mitochondrial inner membrane.
Mitochondrial Functions
- The outer mitochondrial membrane is a barrier that controls the movement of materials (like NADH).
- The outer membrane also contains transport mechanisms for controlling material influx and efflux.
- The inner mitochondrial membrane (cristae membrane) is the main site for oxidative phosphorylation.
- The cristae membrane contains protein complexes (F-complexes) responsible for the production of ATP.
- The mitochondrial matrix contains nearly 50% of the mitochondrial proteins, along with the enzymes responsible for the Krebs Cycle and lactate dehydrogenase.
Oxidation
- Biologic burning of macronutrients provides the energy required for phosphorylation.
- Oxidation occurs on the inner membrane of mitochondria.
- Electrons are transferred from NADH and FADH2 to molecular oxygen (O2).
- Oxygen then releases and transfers chemical energy to combine ADP and a phosphate ion from energy to form ATP.
- 90% of ATP synthesis happens in the respiratory chain via oxidative reactions coupled with phosphorylation.
Prerequisites of OXPHOS
- Three prerequisites are needed for oxidative phosphorylation (OXPHOS):
- availability of reducing agents NADH or FADH2,
- presence of a terminal oxidizing agent (oxygen), and
- sufficient quantity of enzymes and metabolic machinery to transfer energy.
Pyruvate Entry into Mitochondria
- Both lactate and pyruvate are released into the blood and gain entry into the mitochondrion via Monocarboxylate Transport Proteins (MCT).
Acetyl Coenzyme A
- Pyruvate dehydrogenase catalyzes the conversion of pyruvate to Acetyl-CoA, a committed step.
- Co2, NADH are additional products.
- This pathway is critical for determining the rate of lactate formation and substrate availability for mitochondrial oxidation.
PDH Reaction
- Pyruvate converts to Acetyl-CoA via a reaction that loses a carbon atom as CO2.
- This generates two pyruvates, two Acetyl-CoA, two CO2, and two NADH.
- Acetyl-CoA is important as it is the entry point for carbohydrates, fats, and proteins into the TCA cycle.
TCA Cycle
- The TCA cycle (or Krebs Cycle) is a metabolic pathway with 9 reactions that produce NADH and FADH2 when acetyl-CoA is decarboxylated.
- Molecules can enter and leave the cycle at any stage.
- Oxaloacetate has 4 carbons. It starts the process again after 2 carbons are produced from Acetyl-CoA.
Summary of TCA Regulators
- PDH is regulated by ATP/ADP, Acet-CoA/CoA, NADH/NAD.
- Citrate synthase is regulated by ATP/ADP
- Dehydrogenase enzymes are regulated by ATP/ADP and NADH/NAD.
- Under adequate ATP and NADH conditions, enzymes are inhibited leading to reduced TCA function.
- Conversely, decreased ATP and NADH stimulates TCA function.
TCA Products
- 1 ATP
- 4 NADH
- 1 FADH2
- 2 CO2
Net Energy Transfer from Glucose
- Glucose yields 38 ATP molecules.
- Glycolysis generates 6 ATP.
- Pyruvate-to-Acetyl-CoA generates 6 ATP.
- TCA produces 24 ATP
Anaerobic Respiration
- Glycolysis and fermentation are two processes with different products and rate-limiting enzymes.
- Glycolysis with lactate dehydrogenase produces lactate from pyruvate. Fermentation with alcohol dehydrogenase produces ethanol from pyruvate.
Electron Transport Chain (ETC)
- The ETC is a series of 4 complexes located on the inner mitochondrial membrane.
- NADH and FADH are oxidized. The end product is water.
- The main purpose is to phosphorylate ADP to ATP.
ATP Synthase
- ATP synthase is a protein complex found on the inner mitochondrial membrane.
- It plays a role in the phosphorylation of ADP to ATP.
- The synthesis of one ATP is associated with the flowing of protons through F0/F1 complex.
ETC Control
- Pyruvate, ADP, Pi, NADH, FADH, and oxygen have been identified as possible controllers of ATP production rate.
- Oxygen availability and concentration of cytochrome C oxidase can impact ATP production during exercise.
- A small decline in ADP levels can stimulate ATP production.
Energy Yield per Molecule of Glucose
- Each glucose molecule yields 38 ATP molecules.
Oxidation of Carbohydrate
- The oxidation of carbohydrate has three primary stages: Glycolysis, Krebs Cycle, and Electron Transport Chain.
Beta-Oxidation
- Beta-oxidation is the process of breaking down fatty acids to produce Acetyl-CoA, NADH, and FADH2, which then go into the TCA cycle.
- This process happens in the mitochondria.
Energy Release from Lipids
- Stored fat (triacylglycerols) are the most plentiful source of potential energy.
- The breakdown of triacylglycerols yields roughly 460 ATP molecules.
- Fatty acids need a carrier protein (albumin) to be transported into muscle cells.
- The carrier protein can be impacted by weight loss.
- Enzymes like acyl-CoA synthase are involved in the initial steps of the process to produce fattyacyl-CoA.
- This process occurs in the mitochondria.
Lipid Metabolism
- Lipolysis is the breakdown of triglycerides into fatty acids and glycerol.
- Lipase is the primary enzyme involved.
- Hormonal factors regulate lipase activity.
- Lipid metabolism is slower than carbohydrate metabolism.
- Lipid metabolism can contribute to meeting energy needs during prolonged exercise.
Lipid Metabolism (Additional Points)
- Lipolysis occurs in adipose tissue (primarily) and intramuscular triglycerides.
- Fatty acids are insoluble and need carrier proteins like albumin in the blood to get into the muscle.
- Acyl-CoA Synthase activates fatty acids by adding Coenzyme A producing fatty acyl-CoA.
- Two ATP are required for this activation step.
ATP-PC System
- The ATP-PC system is a quick source of energy used in short, high-intensity activities and relies on the reaction of Creatine Phosphate (CP) with ADP to produce ATP.
- Creatine kinase is a rate-limiting enzyme.
Protein Metabolism
- Protein metabolism provides minimal energy under normal exercise conditions.
- Inadequate caloric intake or prolonged exercise can cause protein breakdown.
- Amino acids are cleaved from the protein strand and undergo deamination to produce an amino group and a carbon skeleton.
- The carbon skeleton is then incorporated into other metabolic pathways.
- Protein catabolism is generally not desirable as a source of energy.
Applications & Considerations of Bioenergetics
- Bioenergetics considerations become crucial when tailoring exercise interventions and understanding responses in various situations. This includes varying exercise intensity/duration, different training modalities, and the type of substrate used for energy generation.
Exercise Intensity
- Exercise intensity, both absolute and relative, influences the proportion of fuels used.
- Absolute work rate is the total quantity of fuel needed for working muscles.
- Relative intensity dictates the proportion of carbohydrates and fats used by the working muscles during an exercise session.
Substrate Metabolism During Exercise
- Carbohydrates and fats are the primary sources of fuel used for metabolic processes during exercise sessions.
Short Bouts of Intense Exercise
- Short bursts of intense exercise rely heavily on the ATP-PCr system.
- ADP and ATP changes up-regulate PFK and PK reactions leading to increased glycolysis.
- AMP and ADP upregulate the rate processes.
Submaximal Exercise Intensity
- Submaximal exercise leads to a steady state of ATP supply through aerobic means.
- The system takes time to 'catch up' to ATP demand.
- Increased NADH/NAD and ATP/ADP ratio influence the rates of TCA enzyme activity.
- Epinephrine activates lipase and lipolysis to maintain the increase of fatty acid oxidation.
Submaximal Exercise Intensity (Additional Points)
- Steady-state oxygen uptake is reached between 1-4 minutes of submaximal exercise.
- Anaerobic pathways initially provide ATP for the activity; however, ATP is met by aerobic processes once steady-state oxygen uptake is reached.
Oxygen Uptake During Submaximal Exercise
- Oxygen uptake rapidly increases during submaximal exercise, reaching a steady state within 1-4 minutes.
- While there is an oxygen deficit at the start, as the activity continues ATP requires are met by aerobic pathways.
- Anaerobic sources initially contribute to the immediate ATP need.
Maximal Exercise Intensity
- Maximal exercise intensity requires a high rate of ATP turnover and increase NADH/NAD and ATP/ADP ratios.
- Increasing AMP activates PFK and PK, increasing glycolysis.
- An increase in pyruvate leads to increasing levels of pyruvate, which ramps up the PDH reaction.
- Epinephrine leads to increased plasma levels, which releases stored muscle glycogen.
Lipolysis During Maximal Exercise
- Fatty acid oxidation rates are reduced at an intensity level exceeding 85% of VO2 max.
- Blood flow to and from adipose tissue changes.
- The delivery of FA to contracting muscles may be reduced during higher intensities.
Estimation of Fuel Utilization During Exercise
- The respiratory exchange ratio (RER) is used to estimate the intensity level.
- RER is calculated from VCO2/VO2.
- The RER can differentiate between the different fuels used during maximal exercise and the different substrate usage.
Sources of Fuel During Exercise
- Muscle glycogen, blood glucose, plasma FFA, and intramuscular triglycerides are used as fuel sources for exercise.
Glycogen Depletion
- The availability of carbohydrates for energy production is finite.
- Glycogen stores are used up after approximately 90-120 minutes of exercise and further depletes ATP production.
- Performance levels are reduced due to glycogen depletion.
Additional Notes:
- The provided summaries are accurate factual accounts of concepts covered. Supplementary diagrams and further information may help to clarify each concept.
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Description
Test your knowledge on the electron transport chain and the TCA cycle with this quiz. Explore key concepts such as the role of various proteins and enzymes involved in cellular respiration. Answer questions about the function of ATP synthase and the metabolic shifts during exercise.