Characteristics of Bacteria

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Questions and Answers

What is a major characteristic distinguishing eukaryotic cells from prokaryotic cells?

  • Presence of 70S ribosomes
  • Presence of single, circular DNA genome
  • Size range of 0.5-3.0 μm
  • Presence of a defined nuclear membrane (correct)

Which of the following organisms is classified as prokaryotic?

  • Bacteria (correct)
  • Protozoa
  • Yeast
  • Fungi

What is a common reproductive method for prokaryotic organisms?

  • Asexual division (correct)
  • Recombination
  • Budding
  • Binary fission

Which of the following structures is present in eukaryotic cells but absent in prokaryotic cells?

<p>Mitochondria (D)</p> Signup and view all the answers

What is the size range typically associated with prokaryotic cells?

<p>0.5-3.0 μm (A)</p> Signup and view all the answers

What distinguishes prokaryotes from eukaryotes regarding their cellular structure?

<p>Prokaryotes do not have membrane-bound organelles. (A)</p> Signup and view all the answers

What term describes bacteria that are shaped like rods?

<p>Bacilli (A)</p> Signup and view all the answers

Which step of the Gram stain procedure involves the use of a decolorizer?

<p>Alcohol or acetone treatment (D)</p> Signup and view all the answers

Which of the following statements about viruses is true?

<p>Viruses require host cells for replication. (B)</p> Signup and view all the answers

Which term describes the protective structure formed by some bacteria that allows them to survive unfavorable conditions?

<p>Endospore (B)</p> Signup and view all the answers

What is the primary function of flagella in bacteria?

<p>Locomotion (D)</p> Signup and view all the answers

Which structure is specifically associated with resistance to unfavorable environmental conditions in certain Gram-positive bacteria?

<p>Endospores (C)</p> Signup and view all the answers

What is the composition of bacterial capsules?

<p>Polysaccharides (C)</p> Signup and view all the answers

Which structure is primarily responsible for aiding in the attachment of bacteria to surfaces?

<p>Pili or Fimbriae (C)</p> Signup and view all the answers

Which of the following correctly describes biofilms?

<p>An assemblage of microbial cells enclosed in polysaccharide material (C)</p> Signup and view all the answers

What is NOT a function of capsules in bacteria?

<p>Serving as a nutrient reserve (B)</p> Signup and view all the answers

Which of these structures can remain dormant for extended periods until conditions are favorable?

<p>Endospores (A)</p> Signup and view all the answers

What is the role of pili or fimbriae in bacterial cells?

<p>Attachment to host cells (A)</p> Signup and view all the answers

Which of the following structures are characteristic of bacteria?

<p>Teichoic acid (C), Complex flagellum (D)</p> Signup and view all the answers

Which taxonomic rank is directly below the domain in the classification hierarchy?

<p>Phylum (C)</p> Signup and view all the answers

In scientific nomenclature, how should the species name be written?

<p>Italicized or underlined, not both (C)</p> Signup and view all the answers

What distinguishes a strain of bacteria?

<p>The genetic variants or subtype (D)</p> Signup and view all the answers

What is the primary purpose of serotyping in microbiology?

<p>To distinguish genetic variants of microorganisms (A)</p> Signup and view all the answers

What defines an isolate in microbiological terms?

<p>Specific specimen-derived pure culture (A)</p> Signup and view all the answers

Which of the following statements is true about the classification hierarchy in biology?

<p>Genus consists of related species (B)</p> Signup and view all the answers

What characteristic is NOT associated with Gram-positive bacteria?

<p>Outer lipopolysaccharide layer (A)</p> Signup and view all the answers

Which of the following correctly describes the shape of S.aureus?

<p>Coccus-shaped (A)</p> Signup and view all the answers

Which of the following correctly describes bacterial morphology?

<p>Bacilli can be pleomorphic. (A)</p> Signup and view all the answers

Which component is specifically found in the cell wall of acid-fast bacteria?

<p>Waxy layer of mycolic acids (B)</p> Signup and view all the answers

What is the primary color observed after performing a Gram stain on Gram-negative bacteria?

<p>Red/Pink (B)</p> Signup and view all the answers

Which method is used to classify bacteria into two major classes based on their cell wall structure?

<p>Gram staining (C)</p> Signup and view all the answers

What type of bacterial arrangement is characterized by bacteria forming chains?

<p>Streptococci (C)</p> Signup and view all the answers

What is one of the reagents used in the Gram staining process?

<p>Crystal violet (C)</p> Signup and view all the answers

Which statement is true regarding the comparison between Gram-positive and Gram-negative bacteria?

<p>Gram-positive bacteria lack an outer lipopolysaccharide layer. (A)</p> Signup and view all the answers

What is the generation time for a bacterial population to double from 0 to 2 cells?

<p>0.5 hours (D)</p> Signup and view all the answers

What describes the log or exponential phase of microbial growth?

<p>Maximal growth rate occurs (B)</p> Signup and view all the answers

Which type of microorganism thrives in temperatures above 50-60ºC?

<p>Thermophiles (A)</p> Signup and view all the answers

Which of the following is NOT a chemical requirement for bacterial growth?

<p>Magnesium (B)</p> Signup and view all the answers

What is the role of biofilms in microbial resistance?

<p>They help make bacteria resistant to antimicrobial agents (A)</p> Signup and view all the answers

Which statement best describes the stationary phase of bacterial growth?

<p>The number of cells dying equals the number dividing (D)</p> Signup and view all the answers

What is the significance of hydrogen ion concentration in microbial growth?

<p>It affects how acid-loving bacteria survive (C)</p> Signup and view all the answers

Which component is essential for bacteria to perform metabolic functions?

<p>Nitrogen (D)</p> Signup and view all the answers

Flashcards

Bacteria

Single-celled organisms lacking a nucleus, mitochondria, Golgi bodies, and endoplasmic reticulum. They reproduce asexually.

Eukaryotes

Organisms with a nucleus, mitochondria, Golgi bodies, and endoplasmic reticulum. They can be single-celled or multicellular.

Fungi

A type of eukaryote that can be either single-celled (yeast) or filamentous (mold). They can be parasitic or free-living.

Prokaryote

A type of prokaryote that lacks a nucleus and other internal membrane-bound organelles. They have a single, circular chromosome.

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Nucleus

A structure that contains the genetic material of a eukaryote. It is surrounded by a membrane.

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3 Domain Classification

A classification system dividing life into three domains: Archaea, Bacteria, and Eukarya.

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Cell Wall

A thin, rigid layer surrounding the cell membrane of bacteria, providing structural support and protection.

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Endospore

A specialized structure in some bacteria that allows them to survive harsh conditions.

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Biofilm

A collection of microorganisms living together in a community, usually on a surface.

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Species

A population of cells with similar characteristics, including biochemical makeup, shape, Gram stain reaction, oxygen requirements, and more.

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Family

A group of related genera.

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Genus

Consists of species that differ from each other in certain ways but are related.

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Order

A group of similar families.

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Class

A group of similar orders.

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Phylum

A group of similar classes.

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Domain

The highest level of classification, distinguished by unique molecular analysis.

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Strain

A genetic variant or subtype of a microorganism.

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What are biofilms?

A thin, slimy layer of bacteria that can adhere to surfaces. They are highly resistant to antibiotics and disinfectants due to their protective barrier.

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What are psychrophiles?

Microorganisms that thrive in cold temperatures, with optimal growth between 0°C and 15°C. They can still grow at freezing temperatures.

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What are mesophiles?

Microorganisms that flourish at moderate temperatures, typically between 30°C and 35°C. They are the most common type of bacteria found in humans and animals.

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What are thermophiles?

Microorganisms adapted to hot environments, with optimal growth above 50°C and some even surviving at temperatures above 60°C.

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What is osmosis?

The movement of water across a semipermeable membrane from an area of lower solute concentration to an area of higher solute concentration. It is a key factor affecting microbial growth.

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Sub-culturing a single colony

A process where a single bacterial colony is repeatedly transferred to new growth media, creating many identical cultures.

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Macroscopic Appearance of Bacteria

The visible characteristics of a bacterial colony, such as shape, color, size, and odor.

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What is generation time?

The time it takes for a single bacterial cell to divide into two cells. This is an important indicator of bacterial growth rate.

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What occurs during the lag phase of bacterial growth?

A period of time when bacteria are adjusting to their environment and preparing for growth but are not yet actively dividing.

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Microscopic Appearance of Bacteria

The characteristics of bacteria observed under a microscope, including shape, arrangement, and gram staining.

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Safranin

A dye used in the Gram stain technique, which is a staining method used to differentiate bacterial species based on their cell wall composition.

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Gram Stain Procedure

A staining method used to differentiate bacterial species based on their cell wall composition. It involves four main steps: application of crystal violet, iodine solution, decolorizer, and counterstain (safranin).

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Bacterial Morphology

A classification of bacteria based on their shape. Common types include cocci (spherical), bacilli (rod-shaped), and spirochetes (spiral-shaped).

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What occurs during the log or exponential phase of bacterial growth?

A rapid period of bacterial growth where cells are dividing at their maximum rate. It is a crucial stage for the proliferation of bacteria.

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Gram-Positive Cell Wall

A thick layer of peptidoglycan that forms the cell wall of Gram-positive bacteria.

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Gram-Positive

A type of bacterial cell wall structure that retains the crystal violet dye during the Gram stain procedure, resulting in a purple color under a microscope.

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Gram-Negative

A type of bacterial cell wall structure that loses the initial crystal violet dye during the Gram stain procedure and picks up the counterstain (safranin), resulting in a pink/red color under a microscope.

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Gram-Negative Cell Wall

A thin layer of peptidoglycan, along with an outer membrane containing lipopolysaccharide, which forms the cell wall of Gram-negative bacteria.

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Flagella

Exterior protein filaments that extend from the bacterial cell surface and aid in bacterial movement.

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Gram Stain

A specific stain used to classify bacteria into two major groups: Gram-positive (stain purple) and Gram-negative (stain red/pink).

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Bacterial Growth

A process where bacteria multiply and increase in number under suitable conditions. It is influenced by factors like temperature, nutrient availability, and pH.

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Pili or Fimbriae

Protein fibers extending from the bacterial cell surface that facilitate attachment to surfaces.

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Capsules

Protective outer layers surrounding some bacterial cells. They are typically made of polysaccharides and help bacteria evade the host's immune system.

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Study Notes

Bacterial Characteristics

  • Bacteria are prokaryotic, unicellular organisms
  • Lack a nucleus and membrane-bound organelles like mitochondria, Golgi bodies, and endoplasmic reticulum
  • Reproduce asexually through binary fission
  • Possess a cell wall, cytoplasm, ribosomes, and a nucleoid (DNA) region.

Bacterial Structure

  • Cytoplasm: The jelly-like substance within the cell membrane. Contains essential materials for bacterial metabolism and reproduction.
  • Ribosomes: The protein synthesizing structures within the cytoplasm.
  • Nucleoid: Region of the cytoplasm where the bacterial DNA is located; often a single circular chromosome
  • Cell Wall: A rigid layer providing structural support and protection.
  • Plasma Membrane: A selectively permeable membrane surrounding the cytoplasm. Controls the passage of substances into and out of the cell.
  • Capsule (or Slime Layer): A protective layer external to the cell wall, made of polysaccharides or polypeptides. In some cases, it plays a role in virulence.
  • Flagella: Whip-like appendages used for motility.
  • Pili (Fimbriae): Hair-like structures used for attachment to surfaces.
  • Endospores: Dormant, highly resistant structures formed by some Gram-positive bacteria, allowing survival under harsh conditions. These can create concern for the spread of infection.

Gram-positive Bacteria

  • Thick peptidoglycan layer in the cell wall
  • Teichoic acids are present
  • No outer membrane
  • Usually stain purple in Gram stain procedure due to the thickness of the peptidoglycan layer.

Gram-negative Bacteria

  • Thin peptidoglycan layer in the cell wall
  • Lipopolysaccharide (LPS) as component of outer membrane
  • Outer membrane present
  • Usually stain pink/red in Gram stain procedure as the peptidoglycan layer is thinner and the presence of the outer membrane affects the staining process.

Bacterial Morphology

  • Cocci: Spherical shaped bacteria
  • Bacilli: Rod-shaped bacteria
  • Spirochetes: Spiral-shaped bacteria
  • Pleomorphic: Bacteria that can have various shapes

Classification of Bacteria

  • Three-Domain System: Bacteria and Archaea are prokaryotes, while Eukarya contains eukaryotes.
  • Taxonomy: A hierarchical system used to classify and name microorganisms. Includes categories like Domain, Phylum, Class, Order, Family, Genus, and Species, using binomial nomenclature (e.g., Staphylococcus aureus). Each species has two names that are specific to it, a genus and species name

Microbial Growth

  • Factors influencing bacterial growth: Temperature, osmotic pressure, pH, and chemical requirements (such as oxygen and nutrients).
  • Growth patterns:
    • Lag phase: Initial period of adjustment.
    • Log phase: Exponential growth phase.
    • Stationary phase: Growth rate equals death rate.
    • Death phase: Number of dying cells exceeds number of dividing cells.
  • Binary fission: The primary method of bacterial reproduction. The cell divides to produce two identical daughter cells.

Types of Microorganisms

  • Viruses: The smallest infectious agents. They are not cellular organisms but consist of genetic material (DNA or RNA) surrounded by a protein coat. They replicate inside a host cell.
  • Bacteria: Prokaryotic unicellular organisms.
  • Fungi: Eukaryotic organisms that can be unicellular (yeasts) or multicellular (molds).
  • Parasites: Complex eukaryotic organisms that live off of a host, and may be unicellular or multicellular.
  • Microbiome: The collective microorganisms living in a specific location (including the human body)

Microbial Growth and Isolates

  • Isolate: A pure culture of bacteria obtained from a specific sample.
  • Colony: Clones of bacteria growing on agar medium.
  • Cultural characteristics: External characteristics of microbial colonies, such as color, size, and shape.

Bacterial Structures

  • Flagella: Appendages used for bacterial motility.
  • Pili (Fimbriae): Short, hair-like appendages used for attachment.
  • Capsules: A layer of polysaccharide or polypeptide that protects the bacterium from phagocytosis.
  • Endospores: Highly resistant structures formed by some bacteria for survival under harsh conditions.

Staining of Microorganisms

Methods for differentiating microorganisms include Gram staining (differentiation between Gram-positive and Gram-negative bacteria based on cell wall characteristics)

Microbial Growth Patterns

  • The graph of bacterial growth is typical in presenting phases including lag phase, log phase, stationary phase, death phase.

Other Structures

  • Biofilm: A collection of microorganisms enmeshed in a self-produced matrix, often resistant to antimicrobials.

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