Microbiology Lecture 3: Bacterial Structure

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Questions and Answers

What type of cells are bacterias?

  • Eukaryotic Cells
  • Prokaryotic Cells (correct)
  • Multicellular Organisms
  • None of the above

What type of cells are organisms other than bacteria?

  • Prokaryotic Cells
  • Eukaryotic Cells (correct)
  • Unicellular Organisms
  • None of the above

What are the 3 most common shapes of bacterias?

bacillus, coccus, spirillum

What is a bacillus?

<p>rod-shaped bacterium</p> Signup and view all the answers

What is a coccus?

<p>spherical bacterium</p> Signup and view all the answers

What is a spirillum?

<p>spiral bacterium</p> Signup and view all the answers

What are the major parts of a bacterial cell?

<p>All of the above (D)</p> Signup and view all the answers

What are the three layers that can be found within the envelope of a bacterial cell?

<p>plasma membrane, cell wall, outer membrane</p> Signup and view all the answers

The plasma membrane in bacteria is similar to that in eukaryotic cells, as both consist of a bilayer of __________.

<p>phospholipids</p> Signup and view all the answers

What function does the cell wall serve in bacterial cells?

<p>gives shape and withstands turgor pressure</p> Signup and view all the answers

What is the main component of all bacterial cell walls?

<p>peptidoglycan</p> Signup and view all the answers

What is the role of transpeptidases in bacterial cell walls?

<p>involved in cell wall enlargement</p> Signup and view all the answers

The cell wall of Gram-negative bacteria consists of many layers of peptidoglycan.

<p>False (B)</p> Signup and view all the answers

What is lipid A and where is it found?

<p>a component of LPS in the outer membrane</p> Signup and view all the answers

What is the periplasm in bacteria?

<p>a gelatinous material beneath the outer membrane</p> Signup and view all the answers

What are the components found in the cytoplasm of bacterial cells?

<p>nucleoid, ribosomes, plasmids</p> Signup and view all the answers

What is a nucleoid?

<p>an area containing the DNA of the bacterium</p> Signup and view all the answers

What are ribosomes involved in?

<p>protein synthesis</p> Signup and view all the answers

Flashcards

Prokaryotic Cells

Cells without membrane-bound organelles, like bacteria.

Bacillus

Rod-shaped bacteria; can form chains or clusters.

Coccus

Spherical bacteria; can appear in pairs or clusters.

Spirillum

Spiral-shaped bacteria.

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Plasma Membrane

Phospholipid bilayer in all bacteria; involved in ATP metabolism.

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Cell Wall

Provides shape and rigidity; made of peptidoglycan.

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Peptidoglycan

Composes bacteria's cell wall; consists of glycan and peptide chains.

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Gram-negative Bacteria

Single layer of peptidoglycan; surrounded by an outer membrane.

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Gram-positive Bacteria

Multiple layers of peptidoglycan; thicker walls for strength.

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Transpeptidases

Enzymes that help enlarge the cell wall during growth.

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Penicillin

Antibiotic that inhibits transpeptidases, compromising cell wall.

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Porins

Membrane proteins allowing selective permeability in the outer membrane.

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Lipid A

Part of LPS that acts as an endotoxin when released.

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Endotoxins

Toxins released upon the death of Gram-negative bacteria.

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Periplasm

Gelatinous region between outer and plasma membranes in Gram-negative bacteria.

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Nucleoid

Region in bacteria where the DNA is located, not membrane-bound.

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Ribosomes

Structures for protein synthesis found in all cells.

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Cytoplasm

Less complex than eukaryotic cytoplasm, housing key components like DNA and ribosomes.

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Study Notes

Structure of Bacterial Cells

  • Bacteria are classified as prokaryotic cells, distinguished from eukaryotic organisms which possess membrane-bound organelles.
  • Common bacterial shapes include bacillus (rod-shaped), coccus (spherical), and spirillum (spiral).

Bacterial Shapes

  • Bacillus: Typically exhibits rod-like morphology, can form arrangements such as:
    • Single bacillus
    • Streptobacillus (chains)
    • Coccobacillus (intermediate shape)
  • Coccus: Spherical shape, may appear in:
    • Diplococci (pairs)
    • Streptococci (chains)
    • Staphylococci (clusters)
  • Spirillum: Characterized by spiral forms.

Major Parts of a Bacterial Cell

  • Composed of three major components:
    • Envelope
    • Cytoplasm
    • Structures external to the envelope.

Envelope Composition

  • Plasma Membrane:

    • Present in both Gram-negative and Gram-positive bacteria, and mycoplasmas.
    • Composed of a phospholipid bilayer enriched with proteins involved in ATP metabolism.
  • Cell Wall:

    • Absent in mycoplasmas; provides rigidity and shape to bacteria.
    • Functions to withstand turgor pressure from a hypotonic environment.
    • Composed of peptidoglycan, consisting of:
      • Glycan portion with alternating monosaccharides.
      • Peptido portion containing a peptide of four amino acids linking the chains.
  • Outer Membrane:

    • Unique to Gram-negative bacteria; consists of:
      • Inner layer of phospholipids.
      • Outer layer mainly of lipopolysaccharides (LPS).

Enzymes and Antibiotics

  • Transpeptidases help enlarge the cell wall during bacterial growth; penicillin inhibits these enzymes, affecting cell wall integrity.

Gram Staining Differences

  • Gram-negative bacteria: Have a single layer of peptidoglycan.
  • Gram-positive bacteria: Possess multiple layers of peptidoglycan, contributing to structural strength.

Porins and Permeability

  • The outer membrane is selectively permeable; substances must pass through porins (membrane proteins) for entry.
  • Examples of permeable substances include water and some gases (e.g., O2).

Endotoxins

  • Lipid A, derived from LPS, acts as an endotoxin in humans.
  • Symptoms from endotoxins manifest only when Gram-negative bacteria die, leading to their release during lysis.
  • Initial worsening of symptoms may occur post-antibiotic treatment due to endotoxin release.

Periplasm

  • A region within Gram-negative bacteria, located between the outer membrane and plasma membrane.
  • Filled with gelatinous material and proteins that aid the transport of nutrients into the cell.

Cytoplasm Characteristics

  • Less complex than eukaryotic cytoplasm due to the absence of membrane-bound organelles.
  • Contains essential components such as:
    • Nucleoid (region housing bacterial DNA).
    • Ribosomes (for protein synthesis).
    • Plasmids (circular DNA molecules that can confer additional traits).

Nucleoid and Ribosomes

  • Nucleoid:

    • Contains a single, circular double-stranded DNA molecule known as the bacterial chromosome.
    • DNA is not enclosed by a membrane, differing from eukaryotic cells.
  • Ribosomes:

    • Present in both prokaryotic and eukaryotic cells for protein synthesis; essential for cellular function and growth.

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