Autoradiography and Gel Electrophoresis Quiz
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Questions and Answers

Which method is used to visualize the fragments on the gel?

  • Fluorescence microscopy
  • Immunohistochemistry
  • Electron microscopy
  • Autoradiography (correct)

    • What appears on the X-ray film after autoradiography?

  • Single line
  • Series of bands (correct)
  • No visible image
  • Blotches

    • How can the DNA sequence be read from the autoradiogram?

  • By measuring the size of the bands
  • By interpreting the banding pattern relative to chemical reactions (correct)
  • By counting the number of bands
  • By analyzing the color of the bands

    • What does the presence of a band in the C + T reaction lane indicate?

    <p>Presence of C</p> Signup and view all the answers

    How can sequences be confirmed during the process?

    <p>By running replicate reactions and comparing autoradiographic patterns</p> Signup and view all the answers

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