5 Questions
Which method is used to visualize the fragments on the gel?
Autoradiography
What appears on the X-ray film after autoradiography?
Series of bands
How can the DNA sequence be read from the autoradiogram?
By interpreting the banding pattern relative to chemical reactions
What does the presence of a band in the C + T reaction lane indicate?
Presence of C
How can sequences be confirmed during the process?
By running replicate reactions and comparing autoradiographic patterns
Test your knowledge of autoradiography and gel electrophoresis with this quiz! Learn about how radiolabelled DNA fragments are visualized on X-ray film and how to interpret the bands. Challenge yourself with questions on autoradiographic bands and their representation on the gel.
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