Assessing Sperm Quality and Motility

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Questions and Answers

What is the categorization of sperm motility based on?

Fast progressively motile, slow progressively motile, non-progressively motile and immotile (correct)

Sperm count, sperm morphology, and sperm motility

Sperm head morphology, mid-piece defects, and tail defects

Sperm DNA fragmentation, sperm aneuploidy, and sperm genetics

What is the purpose of the systematic approach in assessing sperm morphology?

To ensure quality assurance and consistent definition of spermatozoa morphology (correct)

To detect sperm aneuploidy and DNA fragmentation

To detect leukocytes in semen samples

To assess sperm motility and count

What is included in the revised chapter on extended examinations?

Only procedures to detect leukocytes and immature germ cells

Procedures to assess sperm morphology and quality control

Biochemical assays for accessory organ function only

Methods to detect sperm aneuploidy, sperm genetics, and DNA fragmentation (correct)

What is the purpose of including micrographs of spermatozoa in the manual?

To train technicians to consistently define subtle features of spermatozoal morphology Signup and view all the answers

What is NOT a classification grade for sperm motility?

Grade e Signup and view all the answers

What is NOT a topic included in the revised chapter on extended examinations?

Sperm capacitation tests Signup and view all the answers

What is the primary focus of the WHO laboratory manual for the examination and processing of human semen?

Sperm morphology and quality control Signup and view all the answers

What is the purpose of quality assurance in sperm morphology assessment?

To ensure consistent definition of spermatozoal morphology Signup and view all the answers

What is the composition of the first fractions of ejaculate?

Sperm-rich prostatic fluids Signup and view all the answers

What influences the volume and content of ejaculates?

Time spent producing a sample by masturbation Signup and view all the answers

What happens to the gel in vitro?

It liquefies due to the action of prostatic proteases Signup and view all the answers

Why do ejaculates produced by masturbation result in lower yield?

Due to a lower level of sexual arousal Signup and view all the answers

What affects the characteristics of ejaculates?

Sperm production by the testes, accessory organ secretions, and other factors Signup and view all the answers

What is the composition of the later fractions of ejaculate?

Sperm-poor seminal vesicular fluid Signup and view all the answers

What is affected by recent febrile illness?

Ejaculate characteristics Signup and view all the answers

What is necessary for accurate laboratory measurements of ejaculate characteristics?

A complete ejaculate collection Signup and view all the answers

What aspects of sexual arousal affect the duration and quality of spermatozoa?

Nerve signals to smooth muscle cells Signup and view all the answers

What is the significance of the initial, sperm-rich prostatic fraction of the ejaculate in vivo?

It comes into contact with cervical mucus extending into the vagina Signup and view all the answers

What is the primary difference between sperm exposure to ejaculatory fluids in vivo and in vitro?

Entire ejaculate collected in one container Signup and view all the answers

What is influenced by the secretory activity of the glands and smooth muscle contractions?

Fluid volume contributed by the accessory glands Signup and view all the answers

What is trapped in a gel developed from proteins of seminal vesicular origin in vitro?

Spermatozoa Signup and view all the answers

What is the result of sexual arousal on sperm function preparation?

Preparation for ejaculation Signup and view all the answers

What is influenced by the composition of the fluids of the ejaculate?

Spermatozoa's function Signup and view all the answers

What is the significance of spermatozoa's vitality, motility, and morphology?

Affects spermatozoa's function Signup and view all the answers

Study Notes

Sperm Motility

Categorization of sperm motility has been revised to include fast progressively motile, slow progressively motile, non-progressively motile, and immotile (grade a, b, c, or d)
Presence or absence of rapid progressive spermatozoa is clinically important

Sperm Morphology

Assessment of sperm morphology should be done using a systematic approach
Micrographs of spermatozoa from unprocessed semen samples are included to help in training technicians to define subtle features of sperm morphology

Sperm Preparation

Ejaculate characteristics can vary depending on the circumstances under which the ejaculate is produced
Ejaculate volume and content of spermatozoa can be affected by the level and duration of sexual arousal
Collection methods, such as masturbation or using non-spermicidal condoms, can result in different yield and characteristics of the ejaculate

Cryopreservation and Quality Assurance

Cryopreservation techniques are available
Quality assurance is essential in the examination and processing of human semen

Ejaculation and Ejaculate Characteristics

Ejaculate characteristics depend on factors such as sperm production, accessory organ secretions, and recent illness
Ejaculation abstinence and other factors can affect laboratory measurements of ejaculate characteristics
A complete ejaculate collection is important for accurate measurements

Ejaculate Composition

The first fractions of the ejaculate are mainly sperm-rich prostatic fluids, while later fractions are dominated by seminal vesicular fluid
The fluid volume contributed by the various accessory glands reflects the secretory activity of the glands and smooth muscle contractions

Sperm Function

The nature of the spermatozoa (vitality, motility, and morphology) and the composition of the ejaculate fluids are important for sperm function
There are differences in sperm exposure to ejaculatory fluids between in vivo and in vitro situations

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Description

This quiz covers the evaluation of sperm motility and morphology, including categorization and quality control measures. Understand the importance of assessing sperm quality in clinical settings.