36 Questions
Which test is used to determine the susceptibility of specific microbes to various antimicrobial drugs?
Kirby-Bauer disk diffusion test
What is the Kirby-Bauer assay?
A test to determine the susceptibility of specific microbes to various antimicrobial drugs
What is observed as a result of the Kirby-Bauer disk diffusion test?
A clear circular zone of inhibition around the drug-impregnated disk
What does the diameter of the zone of inhibition determine in the Kirby-Bauer disk diffusion test?
The susceptibility or resistance of the bacterial pathogen to the drug
What information does the Kirby-Bauer disk diffusion assay provide?
Susceptibility and resistance to the drugs tested
What is the minimal inhibitory concentration (MIC)?
The lowest concentration of drug that inhibits visible bacterial growth
What is the minimal bactericidal concentration (MBC)?
The lowest drug concentration that kills ≥99.9% of the starting inoculum
What tests can be used to determine the minimal inhibitory concentration (MIC)?
Both A and B
What is the Etest?
A test to determine the minimal inhibitory concentration (MIC) of a drug
What can be determined concurrently and directly compared with the Etest?
Minimal inhibitory concentration (MIC)
What is the macrobroth dilution assay?
A test to determine the minimal inhibitory concentration (MIC) of a drug
What is the microdilution tray?
A test to determine the minimal inhibitory concentration (MIC) of a drug
Which test is used to determine the susceptibility of specific microbes to various antimicrobial drugs?
Kirby-Bauer disk diffusion test
What is the Kirby-Bauer assay?
A test to determine the susceptibility of specific microbes to various antimicrobial drugs
What is observed as a result of the Kirby-Bauer disk diffusion test?
A clear circular zone of inhibition around the drug-impregnated disk
What does the diameter of the zone of inhibition determine in the Kirby-Bauer disk diffusion test?
The susceptibility or resistance of the bacterial pathogen to the drug
What information does the Kirby-Bauer disk diffusion assay provide?
Susceptibility and resistance to the drugs tested
What is the minimal inhibitory concentration (MIC)?
The lowest concentration of drug that inhibits visible bacterial growth
What is the minimal bactericidal concentration (MBC)?
The lowest drug concentration that kills ≥99.9% of the starting inoculum
What tests can be used to determine the minimal inhibitory concentration (MIC)?
Both A and B
What is the Etest?
A test to determine the minimal inhibitory concentration (MIC) of a drug
What can be determined concurrently and directly compared with the Etest?
Minimal inhibitory concentration (MIC)
What is the macrobroth dilution assay?
A test to determine the minimal inhibitory concentration (MIC) of a drug
What is the microdilution tray?
A test to determine the minimal inhibitory concentration (MIC) of a drug
Which test is used to determine the susceptibility of specific microbes to various antimicrobial drugs?
The Kirby-Bauer disk diffusion test
What is the purpose of antimicrobial susceptibility testing (AST)?
To identify the spectrum of activity and therapeutic dosage of drugs
What is a confluent lawn in the Kirby-Bauer assay?
A bacterial growth pattern that covers the entire agar plate
What is the zone of inhibition in the Kirby-Bauer assay?
A clear circular area around the drug-impregnated disk where bacterial growth is inhibited
What information can be obtained from the Kirby-Bauer disk diffusion assay?
Limited information on susceptibility and resistance to the drugs tested
What is the minimal inhibitory concentration (MIC)?
The lowest concentration of drug that inhibits visible bacterial growth
What is the minimal bactericidal concentration (MBC)?
The lowest drug concentration that kills ≥99.9% of the starting inoculum
Which tests can be used to determine MICs?
The macrobroth dilution assay and the microdilution tray
What is the Etest?
An alternative method used to determine MIC
What information cannot be obtained from the Etest?
The MBC
What is the macrobroth dilution assay?
A method used to determine MIC by testing bacterial growth in liquid broth with varying drug concentrations
What is the microdilution tray?
A method used to determine MIC by testing bacterial growth in a series of wells with varying drug concentrations
Study Notes
Methods for Testing the Effectiveness of Antimicrobial Drugs
- Antimicrobial susceptibility testing (AST) is important in identifying the spectrum of activity and therapeutic dosage of drugs.
- The Kirby-Bauer disk diffusion test is used to determine the susceptibility of specific microbes to various antimicrobial drugs.
- The Kirby-Bauer assay starts with a Mueller-Hinton agar plate on which a confluent lawn is inoculated with a patient’s isolated bacterial pathogen.
- Filter paper disks impregnated with known amounts of antibacterial drugs are placed on the plate.
- Antibacterial activity is observed as a clear circular zone of inhibition around the drug-impregnated disk.
- The diameter of the zone of inhibition determines the susceptibility or resistance of the bacterial pathogen to the drug.
- The Kirby-Bauer disk diffusion assay provides limited information on susceptibility and resistance to the drugs tested.
- Antibacterial dilution tests can be used to determine a particular drug’s minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC).
- The MIC is the lowest concentration of drug that inhibits visible bacterial growth, while the MBC is the lowest drug concentration that kills ≥99.9% of the starting inoculum.
- The macrobroth dilution assay and the microdilution tray can be used to determine MICs.
- The Etest is an alternative method used to determine MIC, and is a combination of the Kirby-Bauer disk diffusion test and dilution methods.
- The MIC of multiple antimicrobials can be determined concurrently and directly compared with the Etest, but the MBC cannot be determined.
Test your knowledge on the methods used to evaluate the effectiveness of antimicrobial drugs with this quiz. From the Kirby-Bauer disk diffusion test to antibacterial dilution tests, this quiz will cover the essential techniques used to determine a drug's ability to inhibit or kill bacterial pathogens. Brush up on your understanding of antimicrobial susceptibility testing, minimal inhibitory concentration, and more with this informative quiz. Perfect for students, healthcare professionals, and anyone interested in microbiology.
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