Antimicrobial Susceptibility Testing Quiz
36 Questions
1 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

Which test is used to determine the susceptibility of specific microbes to various antimicrobial drugs?

  • Etest
  • Kirby-Bauer disk diffusion test (correct)
  • Antimicrobial susceptibility testing (AST)
  • Antibacterial dilution tests

    • What is the Kirby-Bauer assay?

  • A test to determine the minimal inhibitory concentration (MIC) of a drug
  • A test to determine the minimal bactericidal concentration (MBC) of a drug
  • A test to determine the effectiveness of antibiotics in treating viral infections
  • A test to determine the susceptibility of specific microbes to various antimicrobial drugs (correct)

    • What is observed as a result of the Kirby-Bauer disk diffusion test?

  • Minimal inhibitory concentration (MIC)
  • Antibacterial resistance
  • A clear circular zone of inhibition around the drug-impregnated disk (correct)
  • Bacterial growth

    • What does the diameter of the zone of inhibition determine in the Kirby-Bauer disk diffusion test?

    <p>The susceptibility or resistance of the bacterial pathogen to the drug</p> Signup and view all the answers

    What information does the Kirby-Bauer disk diffusion assay provide?

    <p>Susceptibility and resistance to the drugs tested</p> Signup and view all the answers

    What is the minimal inhibitory concentration (MIC)?

    <p>The lowest concentration of drug that inhibits visible bacterial growth</p> Signup and view all the answers

    What is the minimal bactericidal concentration (MBC)?

    <p>The lowest drug concentration that kills ≥99.9% of the starting inoculum</p> Signup and view all the answers

    What tests can be used to determine the minimal inhibitory concentration (MIC)?

    <p>Both A and B</p> Signup and view all the answers

    What is the Etest?

    <p>A test to determine the minimal inhibitory concentration (MIC) of a drug</p> Signup and view all the answers

    What can be determined concurrently and directly compared with the Etest?

    <p>Minimal inhibitory concentration (MIC)</p> Signup and view all the answers

    What is the macrobroth dilution assay?

    <p>A test to determine the minimal inhibitory concentration (MIC) of a drug</p> Signup and view all the answers

    What is the microdilution tray?

    <p>A test to determine the minimal inhibitory concentration (MIC) of a drug</p> Signup and view all the answers

    Which test is used to determine the susceptibility of specific microbes to various antimicrobial drugs?

    <p>Kirby-Bauer disk diffusion test</p> Signup and view all the answers

    What is the Kirby-Bauer assay?

    <p>A test to determine the susceptibility of specific microbes to various antimicrobial drugs</p> Signup and view all the answers

    What is observed as a result of the Kirby-Bauer disk diffusion test?

    <p>A clear circular zone of inhibition around the drug-impregnated disk</p> Signup and view all the answers

    What does the diameter of the zone of inhibition determine in the Kirby-Bauer disk diffusion test?

    <p>The susceptibility or resistance of the bacterial pathogen to the drug</p> Signup and view all the answers

    What information does the Kirby-Bauer disk diffusion assay provide?

    <p>Susceptibility and resistance to the drugs tested</p> Signup and view all the answers

    What is the minimal inhibitory concentration (MIC)?

    <p>The lowest concentration of drug that inhibits visible bacterial growth</p> Signup and view all the answers

    What is the minimal bactericidal concentration (MBC)?

    <p>The lowest drug concentration that kills ≥99.9% of the starting inoculum</p> Signup and view all the answers

    What tests can be used to determine the minimal inhibitory concentration (MIC)?

    <p>Both A and B</p> Signup and view all the answers

    What is the Etest?

    <p>A test to determine the minimal inhibitory concentration (MIC) of a drug</p> Signup and view all the answers

    What can be determined concurrently and directly compared with the Etest?

    <p>Minimal inhibitory concentration (MIC)</p> Signup and view all the answers

    What is the macrobroth dilution assay?

    <p>A test to determine the minimal inhibitory concentration (MIC) of a drug</p> Signup and view all the answers

    What is the microdilution tray?

    <p>A test to determine the minimal inhibitory concentration (MIC) of a drug</p> Signup and view all the answers

    Which test is used to determine the susceptibility of specific microbes to various antimicrobial drugs?

    <p>The Kirby-Bauer disk diffusion test</p> Signup and view all the answers

    What is the purpose of antimicrobial susceptibility testing (AST)?

    <p>To identify the spectrum of activity and therapeutic dosage of drugs</p> Signup and view all the answers

    What is a confluent lawn in the Kirby-Bauer assay?

    <p>A bacterial growth pattern that covers the entire agar plate</p> Signup and view all the answers

    What is the zone of inhibition in the Kirby-Bauer assay?

    <p>A clear circular area around the drug-impregnated disk where bacterial growth is inhibited</p> Signup and view all the answers

    What information can be obtained from the Kirby-Bauer disk diffusion assay?

    <p>Limited information on susceptibility and resistance to the drugs tested</p> Signup and view all the answers

    What is the minimal inhibitory concentration (MIC)?

    <p>The lowest concentration of drug that inhibits visible bacterial growth</p> Signup and view all the answers

    What is the minimal bactericidal concentration (MBC)?

    <p>The lowest drug concentration that kills ≥99.9% of the starting inoculum</p> Signup and view all the answers

    Which tests can be used to determine MICs?

    <p>The macrobroth dilution assay and the microdilution tray</p> Signup and view all the answers

    What is the Etest?

    <p>An alternative method used to determine MIC</p> Signup and view all the answers

    What information cannot be obtained from the Etest?

    <p>The MBC</p> Signup and view all the answers

    What is the macrobroth dilution assay?

    <p>A method used to determine MIC by testing bacterial growth in liquid broth with varying drug concentrations</p> Signup and view all the answers

    What is the microdilution tray?

    <p>A method used to determine MIC by testing bacterial growth in a series of wells with varying drug concentrations</p> Signup and view all the answers

    Study Notes

    Methods for Testing the Effectiveness of Antimicrobial Drugs

    • Antimicrobial susceptibility testing (AST) is important in identifying the spectrum of activity and therapeutic dosage of drugs.
    • The Kirby-Bauer disk diffusion test is used to determine the susceptibility of specific microbes to various antimicrobial drugs.
    • The Kirby-Bauer assay starts with a Mueller-Hinton agar plate on which a confluent lawn is inoculated with a patient’s isolated bacterial pathogen.
    • Filter paper disks impregnated with known amounts of antibacterial drugs are placed on the plate.
    • Antibacterial activity is observed as a clear circular zone of inhibition around the drug-impregnated disk.
    • The diameter of the zone of inhibition determines the susceptibility or resistance of the bacterial pathogen to the drug.
    • The Kirby-Bauer disk diffusion assay provides limited information on susceptibility and resistance to the drugs tested.
    • Antibacterial dilution tests can be used to determine a particular drug’s minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC).
    • The MIC is the lowest concentration of drug that inhibits visible bacterial growth, while the MBC is the lowest drug concentration that kills ≥99.9% of the starting inoculum.
    • The macrobroth dilution assay and the microdilution tray can be used to determine MICs.
    • The Etest is an alternative method used to determine MIC, and is a combination of the Kirby-Bauer disk diffusion test and dilution methods.
    • The MIC of multiple antimicrobials can be determined concurrently and directly compared with the Etest, but the MBC cannot be determined.

    Studying That Suits You

    Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

    Quiz Team

    Description

    Test your knowledge on the methods used to evaluate the effectiveness of antimicrobial drugs with this quiz. From the Kirby-Bauer disk diffusion test to antibacterial dilution tests, this quiz will cover the essential techniques used to determine a drug's ability to inhibit or kill bacterial pathogens. Brush up on your understanding of antimicrobial susceptibility testing, minimal inhibitory concentration, and more with this informative quiz. Perfect for students, healthcare professionals, and anyone interested in microbiology.

    More Like This

    Use Quizgecko on...
    Browser
    Open
    Browser
    Browser