Microbiology Commercial Testing Methods Quiz

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Which of the following is NOT a method for conducting and evaluating antimicrobial susceptibility testing?

Time-kill studies (correct)

Disk diffusion

Broth dilution

Agar dilution

Which method directly measures the activity of antimicrobial agents against a bacterial isolate?

Agar dilution

Time-kill studies

Broth dilution (correct)

Disk diffusion

Which method directly detects the presence of a specific resistance mechanism in a bacterial isolate?

Agar dilution

Broth dilution

Disk diffusion

β-Lactamase detection (correct)

Which method measures complex antimicrobial-organism interactions?

Serum Bactericidal (Schlichter Test) Signup and view all the answers

Which of the following is NOT a conventional susceptibility testing method?

E-test Signup and view all the answers

Which method is used to detect and evaluate the accuracy of the used strategy in antimicrobial susceptibility testing?

Predictor Antimicrobial Agents Signup and view all the answers

Which method of conventional testing uses a range of concentrations of antimicrobial agent in liquid environment?

Broth Dilution Signup and view all the answers

What is the broth volume used for microdilution testing?

0.05 to 0.1 mL Signup and view all the answers

What is the standardized bacterial concentration in each microtiter well for microdilution testing?

5 x 105 CFU/mL Signup and view all the answers

What is the purpose of the internal control called 'Growth control' in conventional testing methods?

Detect positive growth Signup and view all the answers

What does the abbreviation 'MIC' stand for in conventional testing methods?

Minimum Inhibition Concentration Signup and view all the answers

Which conventional testing method uses antibiotic disks on the surface of an agar plate seeded with bacteria?

Disk Diffusion Signup and view all the answers

Which method is used to detect bactericidal activity of an antimicrobial agent?

Time-kill Studies Signup and view all the answers

Which of the following is NOT a method for quality control of antimicrobial susceptibility tests?

Consecutive testing Signup and view all the answers

What is the interpretation of a 1000-fold decrease in the number of viable bacteria in the antibiotic-containing broth after a 24-hour period, compared with the number of bacteria in the original inoculum?

Bactericidal activity Signup and view all the answers

What is the purpose of serum-bactericidal testing (SBT)/Schlichter Test?

To detect bactericidal activity of an antimicrobial agent Signup and view all the answers

What is the most commonly used method for determining the rate of killing of bacteria over time?

Time-kill Studies Signup and view all the answers

What is the purpose of antibiograms?

To determine the overall antimicrobial susceptibility profile of an organism Signup and view all the answers

Which method is most commonly used to test for the presence of β-lactamase enzymes?

Chromogenic cephalosporinase test Signup and view all the answers

What is the purpose of the D-test in the context of macrolide-lincosamide-streptograminB resistance?

To distinguish between efflux and iMLSB mechanisms of resistance Signup and view all the answers

Which gene is responsible for intrinsic resistance to vancomycin in motile and pigmented Enterococcal species?

van C gene Signup and view all the answers

What is the purpose of the Carba NP test?

To detect the presence of carbapenemase production in Enterobacteriaceae Signup and view all the answers

Which resistance mechanism is associated with the presence of the erm gene?

iMLSB mechanism Signup and view all the answers

Which method directly measures the activity of antimicrobial agents against a bacterial isolate?

Phenotypic methods Signup and view all the answers

Which method combines the convenience of disk diffusion with the ability to generate MIC data?

Diffusion in Agar Derivations Signup and view all the answers

Which system uses optical readings every 15 minutes to measure the amount of light transmitted through each well to derive the MIC data?

Vitek 2 Signup and view all the answers

Which system provides susceptibility results in 3.5 to 5.5 hours by using the degradation of fluorogenic substrates by viable bacteria?

MicroScan WalkAway Signup and view all the answers

Which system uses a redox indicator system to monitor growth and can perform supplemental testing such as the confirmatory extended spectrum beta-lactamase test for E. coli?

Phoenix Signup and view all the answers

Which method can be used to detect oxacillin resistance in S. aureus and S. lugdunensis by growth in 4µg/ml cefoxitin?

Broth dilution method Signup and view all the answers

What do inhibitory zones of ≤ 21 mm indicate in the context of oxacillin resistance in S. aureus and S. lugdunensis?

Oxacillin resistance Signup and view all the answers

Which method is used to detect the presence of β-lactamase enzymes?

Disk diffusion Signup and view all the answers

What is the purpose of the Carba NP test?

To detect carbapenemase production Signup and view all the answers

What does the abbreviation 'MIC' stand for in conventional testing methods?

Minimal Inhibitory Concentration Signup and view all the answers

Which system uses optical readings every 15 minutes to measure the amount of light transmitted through each well to derive the MIC data?

Automated antimicrobial susceptibility testing systems Signup and view all the answers

Which conventional testing method uses antibiotic disks on the surface of an agar plate seeded with bacteria?

Disk diffusion Signup and view all the answers

Which method is used to detect and evaluate the accuracy of the used strategy in antimicrobial susceptibility testing?

Predictor Signup and view all the answers

Which method is used to detect the presence of the mecA gene in bacteria?

Nucleic acid hybridization Signup and view all the answers

What is the purpose of the D-test in the context of macrolide-lincosamide-streptograminB resistance?

To distinguish between efflux and iMLSB mechanisms of resistance Signup and view all the answers

Which resistance mechanism is associated with the presence of the vanA or vanB gene?

Acquired resistance Signup and view all the answers

Which method is used to detect carbapenemase production in species belonging to Enterobacteriaceae, P. aeruginosa, and Acinetobacter?

Carba NP test Signup and view all the answers

Which method is most commonly used to test for the presence of β-lactamase enzymes?

Chromogenic cephalosporinase test Signup and view all the answers

Which method directly measures the activity of antimicrobial agents against a bacterial isolate?

Disc diffusion Signup and view all the answers

Which method is commonly used to determine the minimum inhibitory concentration (MIC) of an antimicrobial agent in a liquid environment?

Broth dilution Signup and view all the answers

Which method is NOT suitable for testing slow-growing organisms such as mycobacteria and anaerobes?

Disk diffusion Signup and view all the answers

What is the purpose of the internal control called 'Growth control' in conventional testing methods?

To detect positive growth in the absence of an antimicrobial agent Signup and view all the answers

What is the purpose of the D-test in the context of macrolide-lincosamide-streptograminB resistance?

To detect the presence of a specific resistance mechanism in a bacterial isolate Signup and view all the answers

What does the abbreviation 'MIC' stand for in conventional testing methods?

Minimum Inhibitory Concentration Signup and view all the answers

What is the standardized bacterial concentration in each microtiter well for microdilution testing?

5 x 10^5 CFU/mL Signup and view all the answers

Which method is used to detect bactericidal activity of an antimicrobial agent?

Time-kill Studies Signup and view all the answers

What is the interpretation of a 1000-fold decrease in the number of viable bacteria in the antibiotic-containing broth after a 24-hour period, compared with the number of bacteria in the original inoculum?

Bactericidal activity Signup and view all the answers

Which of the following is NOT a method for quality control of antimicrobial susceptibility tests?

Periodic testing Signup and view all the answers

What is the purpose of the Carba NP test?

To detect the presence of carbapenemase enzymes Signup and view all the answers

Which method measures complex antimicrobial-organism interactions?

Serum-bactericidal testing (SBT)/Schlichter Test Signup and view all the answers

Which system uses a redox indicator system to monitor growth and can perform supplemental testing such as the confirmatory extended spectrum beta-lactamase test for E. coli?

VITEK 2 system Signup and view all the answers

Which method combines the convenience of disk diffusion with the ability to generate MIC data?

Diffusion in Agar Derivations Signup and view all the answers

Which system provides susceptibility results in 3.5 to 5.5 hours by using the degradation of fluorogenic substrates by viable bacteria?

MicroScan WalkAway system Signup and view all the answers

What is the purpose of the D-test in the context of macrolide-lincosamide-streptograminB resistance?

To detect inducible clindamycin resistance in Staphylococcus species Signup and view all the answers

Which method can be used to detect oxacillin resistance in S. aureus and S. lugdunensis by growth in 4µg/ml cefoxitin?

Broth microdilution method Signup and view all the answers

Which system uses optical readings every 15 minutes to measure the amount of light transmitted through each well to derive the MIC data?

Vitek Legacy and Vitek 2 systems Signup and view all the answers

What do inhibitory zones of ≤ 21 mm indicate in the context of oxacillin resistance in S. aureus and S. lugdunensis?

Presence of oxacillin (or methicillin) resistance Signup and view all the answers

Microbiology Commercial Testing Methods Quiz

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Questions and Answers

Which of the following is NOT a method for conducting and evaluating antimicrobial susceptibility testing?

Which method directly measures the activity of antimicrobial agents against a bacterial isolate?

Which method directly detects the presence of a specific resistance mechanism in a bacterial isolate?

Which method measures complex antimicrobial-organism interactions?

Which of the following is NOT a conventional susceptibility testing method?

Which method is used to detect and evaluate the accuracy of the used strategy in antimicrobial susceptibility testing?

Which method of conventional testing uses a range of concentrations of antimicrobial agent in liquid environment?

What is the broth volume used for microdilution testing?

What is the standardized bacterial concentration in each microtiter well for microdilution testing?

What is the purpose of the internal control called 'Growth control' in conventional testing methods?

What does the abbreviation 'MIC' stand for in conventional testing methods?

Which conventional testing method uses antibiotic disks on the surface of an agar plate seeded with bacteria?

Which method is used to detect bactericidal activity of an antimicrobial agent?

Which of the following is NOT a method for quality control of antimicrobial susceptibility tests?

What is the interpretation of a 1000-fold decrease in the number of viable bacteria in the antibiotic-containing broth after a 24-hour period, compared with the number of bacteria in the original inoculum?

What is the purpose of serum-bactericidal testing (SBT)/Schlichter Test?

What is the most commonly used method for determining the rate of killing of bacteria over time?

What is the purpose of antibiograms?

Which method is most commonly used to test for the presence of β-lactamase enzymes?

What is the purpose of the D-test in the context of macrolide-lincosamide-streptograminB resistance?

Which gene is responsible for intrinsic resistance to vancomycin in motile and pigmented Enterococcal species?

What is the purpose of the Carba NP test?

Which resistance mechanism is associated with the presence of the erm gene?

Which method directly measures the activity of antimicrobial agents against a bacterial isolate?

Which method combines the convenience of disk diffusion with the ability to generate MIC data?

Which system uses optical readings every 15 minutes to measure the amount of light transmitted through each well to derive the MIC data?

Which system provides susceptibility results in 3.5 to 5.5 hours by using the degradation of fluorogenic substrates by viable bacteria?

Which system uses a redox indicator system to monitor growth and can perform supplemental testing such as the confirmatory extended spectrum beta-lactamase test for E. coli?

Which method can be used to detect oxacillin resistance in S. aureus and S. lugdunensis by growth in 4µg/ml cefoxitin?

What do inhibitory zones of ≤ 21 mm indicate in the context of oxacillin resistance in S. aureus and S. lugdunensis?

Which method is used to detect the presence of β-lactamase enzymes?

What is the purpose of the Carba NP test?

What does the abbreviation 'MIC' stand for in conventional testing methods?

Which system uses optical readings every 15 minutes to measure the amount of light transmitted through each well to derive the MIC data?

Which conventional testing method uses antibiotic disks on the surface of an agar plate seeded with bacteria?

Which method is used to detect and evaluate the accuracy of the used strategy in antimicrobial susceptibility testing?

Which method is used to detect the presence of the mecA gene in bacteria?

What is the purpose of the D-test in the context of macrolide-lincosamide-streptograminB resistance?

Which resistance mechanism is associated with the presence of the vanA or vanB gene?

Which method is used to detect carbapenemase production in species belonging to Enterobacteriaceae, P. aeruginosa, and Acinetobacter?

Which method is most commonly used to test for the presence of β-lactamase enzymes?

Which method directly measures the activity of antimicrobial agents against a bacterial isolate?

Which method is commonly used to determine the minimum inhibitory concentration (MIC) of an antimicrobial agent in a liquid environment?

Which method is NOT suitable for testing slow-growing organisms such as mycobacteria and anaerobes?

What is the purpose of the internal control called 'Growth control' in conventional testing methods?

What is the purpose of the D-test in the context of macrolide-lincosamide-streptograminB resistance?

What does the abbreviation 'MIC' stand for in conventional testing methods?

What is the standardized bacterial concentration in each microtiter well for microdilution testing?

Which method is used to detect bactericidal activity of an antimicrobial agent?

What is the interpretation of a 1000-fold decrease in the number of viable bacteria in the antibiotic-containing broth after a 24-hour period, compared with the number of bacteria in the original inoculum?

Which of the following is NOT a method for quality control of antimicrobial susceptibility tests?

What is the purpose of the Carba NP test?

Which method measures complex antimicrobial-organism interactions?

Which system uses a redox indicator system to monitor growth and can perform supplemental testing such as the confirmatory extended spectrum beta-lactamase test for E. coli?

Which method combines the convenience of disk diffusion with the ability to generate MIC data?

Which system provides susceptibility results in 3.5 to 5.5 hours by using the degradation of fluorogenic substrates by viable bacteria?

What is the purpose of the D-test in the context of macrolide-lincosamide-streptograminB resistance?

Which method can be used to detect oxacillin resistance in S. aureus and S. lugdunensis by growth in 4µg/ml cefoxitin?

Which system uses optical readings every 15 minutes to measure the amount of light transmitted through each well to derive the MIC data?

What do inhibitory zones of ≤ 21 mm indicate in the context of oxacillin resistance in S. aureus and S. lugdunensis?

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