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Questions and Answers
Where are antibodies found?
Where are antibodies found?
plasma of the blood
How can antibodies be used in the laboratory?
How can antibodies be used in the laboratory?
They can be used to see if the patient has a disease that is specifically caused by infections or a malfunction in the immune system.
What does ELISA stand for?
What does ELISA stand for?
enzyme-linked immunosorbent assay
What are ELISA assays used for in labs?
What are ELISA assays used for in labs?
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What are the three important limitations of an ELISA?
What are the three important limitations of an ELISA?
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What does a positive result indicate in an ELISA test?
What does a positive result indicate in an ELISA test?
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When is detection possible in an ELISA test?
When is detection possible in an ELISA test?
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What is the ELISA protocol?
What is the ELISA protocol?
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Study Notes
Antibodies
- Found in the plasma of the blood.
- Serve as critical components of the immune response.
Laboratory Use of Antibodies
- Utilized to detect diseases caused by infections or immune system malfunctions.
- Indicate the presence of specific antibodies in patients.
ELISA Definition
- Stands for enzyme-linked immunosorbent assay.
- A vital laboratory technique for antibody or antigen detection.
Purpose of ELISA Assays
- Measure concentrations of antibodies or antigens in samples.
- Help diagnose diseases or determine immune responses.
Limitations of ELISA
- A positive result for antibodies does not confirm illness; the body may produce antibodies after recovery.
- Inadequate antibody production may lead to false negatives.
- Non-specific antigen binding can result in misleading positive outcomes, affecting test reliability.
Positive Result Interpretation
- Indicates the presence of antibodies correlating with an active disease in the individual.
Detection Method
- Achieved by adding a second antibody that produces a detectable signal.
ELISA Protocol Steps
- Bind sample to support, add primary antibody, wash to remove unbound material.
- Add secondary antibody, wash again, then add substrate to visualize the test result.
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Description
This quiz covers essential topics about antibodies and the ELISA method used in laboratories. You will explore the role of antibodies in the immune system, the functionality of ELISA assays, and their limitations. Test your understanding of how these components are utilized in disease diagnosis.
